Figure 2
Example 2Fo − Fc maps at a contour level of 1σ for (a) HEWL, (b) DtpAa (eight drops, 5360 merged diffraction patterns), (c) DtpAa (12 drops, 10 054 merged diffraction patterns) and (d) DtpAa (35 drops, 22 854 merged diffraction patterns). Note that all Fe—O distances are the same within error (shown in parentheses next to the bond length; Kumar et al., 2015). The structures of DtpAa used previous models as a starting point for refinement (Ebrahim et al., 2019) and the active site of chain A is shown. Fo − Fc omit maps showing the presence of clearly defined waters without model bias are included in the supporting information. |