journal menu
![[Figure 2]](jb5069fig2mag.jpg)
|
|
Figure 2
Cryo-EM density map and model fitting of the hexameric ArnA complex. (a) Cryo-EM density map of the ArnA hexamer reconstructed at 4.0 Å resolution, shown from three orientations: top, side and bottom views. The map reveals the characteristic two-layered architecture of ArnA and clearly resolved secondary-structure elements. (b) Final atomic model of ArnA fitted into the cryo-EM density map. Each of the 12 subunits is displayed in a different color to illustrate the hexameric arrangement. (c) Structural alignment of the final cryo-EM model (blue) with the reference crystal structure (yellow) using ChimeraX. The alignment yielded an r.m.s.d. of 1.2 Å. Enlarged views show local conformational deviations in loop regions: Glu69–Ala98 in chain D (left) and Pro65–Ser75 in chain F (right). (d) Close-up view showing the model-to-map fit for a peptide segment (Leu483–Arg460). The density mesh is contoured at 1.7σ, showing clear peptide backbone density and supporting accurate model placement. |
![[Figure 2]](jb5069fig2.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
Open 
access
access
