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Figure 2
Crystals of 1TEL–DARPin constructs with flexible, semi-flexible and rigid linkers. Representative crystals obtained for all 1TEL–DARPin constructs, both with (10×His Tag) and without (No Tag) the His-tag. Constructs outlined in black represent those selected for further optimization, resulting in better quality crystals. Crystals from those optimizations are shown in the offset box at the top right. The boxed area highlights representative optimized crystals obtained from three constructs: 1TEL-PA-DARPin, 10×His-1TEL-short-rigid-DARPin and 10×His-1TEL-medium-rigid-DARPin. Images were captured using SeBaView (Laxco) and figures were prepared using Microsoft PowerPoint.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
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