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![[Figure 7]](chr5009fig7mag.jpg)
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Figure 7
Analysis of 1TEL–UBA linkers and superposition of rigid, semi-flexible and flexible 1TEL–DARPin and 3TEL–DARPin fusions. (a) Superposition of a theoretical Pro-Ala linker between a 1TEL (slate) and a UBA (cyan) from the published structure of 1TEL-GG-UBA (PDB entry 7tdy) with clashes shown in spheres. (b) As in (a), but for the published PDB entry 7tdy with its original Gly-Gly linker. (c) Structures of constructs with rigid linkers: 1TEL-medium rigid-DARPin (PDB entry 9dp8, cyan), `3-fold' 1TEL-short-rigid-DARPin (PDB entry 9znb, yellow), `2-fold' 1TEL-short-rigid-DARPin (PDB entry 9dvg, pink) and 3TEL-long-rigid-DARPin (PDB entry 7n2b, blue), superimposed (via the Cα atoms of their 1TEL domains) onto a 1TEL polymer (light grey). (d) Structures of constructs with semi-flexible and flexible linkers, 1TEL-PA-DARPin (PDB entry 9db5, green) and 3TEL-GGG-DARPin (PDB entry 9e4q, orange), superimposed (via the Cα atoms of their C-terminal-most 1TEL domain) onto a 1TEL polymer (light grey). (e) Superposition of (c) and (d). Figures were prepared using PyMOL (Schrödinger) and Microsoft PowerPoint. |
![[Figure 7]](chr5009fig7.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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