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![[Figure 1]](gm5122fig1mag.jpg)
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Figure 1
Experimental setup for UV–Vis absorption spectroscopy acquisition with examples. (a) The loop with the sample was mounted on the goniometer at ESRF beamline BM07-FIP2 in the cryostream and was not rotated during X-ray exposure; the UV–Vis absorption microspectrophotometer lamp and sensor were aligned with the sample. Inset: on-axis view with the top-hat X-ray beam (200 × 200 µm in all experiments) passing through the on-axis camera objective pinhole onto the mounted sample, with the light path orthogonal to the X-ray beam. (b) On-axis images of a mounted loop, here 30% glycerol. Left: thickness is determined with the loop edge-on. Right: the face-on view allows evaluation of the sample transparency, shown here before and after X-ray exposure. (c) Flash-cooling of 20%(w/v) glucose resulted in a transparent thin film, but 20%(w/v) sucrose did not, highlighting challenges in producing homogeneous vitreous films. (d) The Morpheus halide additive mixture is an example of a solution that did not lead to a transparent vitreous film upon flash-cooling and additionally showed visible changes after X-ray exposure. |
![[Figure 1]](gm5122fig1.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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