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![[Figure 5]](gm5122fig5mag.jpg)
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Figure 5
Effect of X-ray irradiation on commonly used cryoprotectants. (a) The distinct transient UV–Vis absorption peak at 540 nm arises from light-absorbing solvated electrons in 20% glycerol. (b) With 100% ethylene glycol, the solvated electron peak similarly appears with a maximum at 535 nm. (c) The solvated electron peak is less pronounced in saccharides such as glucose or sucrose, while the signal intensity reduces at lower concentrations of the saccharides, with maxima between 560 and 590 nm at the measured concentrations of 20% and 50%. (d) 2-Methyl-2,4-pentanediol (MPD) exhibits a transient peak near 600 nm at 50%, which is absent at 25%(w/v) concentration. The spectra series are represented from red (start) to blue (end). |
![[Figure 5]](gm5122fig5.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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