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Figure 3
Network diagram and pull-down assays for the gynoecium interactome. (a) Dimer, trimer and tetramer complexes queued for computation from yeast two-hybrid data. Red denotes poisoned complexes based on an ipTM cutoff of 0.5. Dimers (squares), trimers (circles) and tetramers (triangles) are shown and are colored by ipTM, with darker blue denoting higher ipTM scores. (b) Pull-down experiment demonstrating interaction between gynoecium dimers. All proteins were expressed using in vitro transcription translation and different N- or C-terminal tags. (c) Pull-down experiment demonstrating interaction between gynoecium trimers. SPT was poorly expressed and its interactions with AS2 and IND could not be confirmed.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
Volume 82| Part 5| May 2026| Pages 421-433
https://doi.org/10.1107/S2059798326003013
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