view article
Figure 4
Network diagram and pull-down assays for the mTERF9 interactome. (a) Dimer, trimer and tetramer complexes queued for computation. Red denotes poisoned complexes based on an ipTM cutoff of 0.5. Dimers (squares), trimers (circles) and tetramers (triangles) are shown and are colored by ipTM, with darker blue denoting higher ipTM scores. (b) Pull-down experiment demonstrating interaction between high-scoring mTERF9 dimers. All proteins were expressed using in vitro transcription translation with an N-terminal 3×FLAG tag for mTERF9 and a 5×Myc tag for the different prey proteins. RSP, RSP19; US10, US10Y; CF1, ATHCF1β. (c) Pull-down experiment demonstrating interaction between an mTERF9 trimer. RSP19 was poorly expressed alone but was easily detectable when co-expressed and in the pull-down assay.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
Volume 82| Part 5| May 2026| Pages 421-433
https://doi.org/10.1107/S2059798326003013
Follow Acta Cryst. D
Sign up for e-alerts
E-alerts
Follow Acta Cryst. on Twitter
Twitter
Follow us on facebook
Facebook
Sign up for RSS feeds
RSS