Generation and modulation of catalytically relevant states of a dye-decolourizing peroxidase using time-resolved serial femtosecond crystallography with drop-on-chip mixing and X-ray-driven reduction

Lučić, M.; Glerup, J.; Aller, P.; Axford, D.; Devenish, N.; Park, J.; Shilova, A.; Landeros de la Isla, A.; Strange, R.W.; Zhou, T.; Owen, R.L.; Worrall, J.A.R.; Hough, M.A.

doi:10.1107/S2059798326001658

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

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Figure 4
Occupancy of the iron-coordinated O atom versus post-mixing time and accumulated dose for each chain of the DtpB hexamer. (a) Time-dependent change in O-atom occupancy in individual DtpB chains over the time course of data collection shown in Fig. 3

. (b) Dose-dependent changes in O-atom and H₂O occupancy for the initially iron-coordinated O atom (Fig. 5

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 82| Part 5| May 2026| Pages 434-445

https://doi.org/10.1107/S2059798326001658

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