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![[Figure 3]](zi5010fig3mag.jpg)
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Figure 3
MiCSPARC reconstruction of an undecorated, dynamic microtubule. (a) Initial rough signal-subtracted reconstruction of a single protofilament (left), which was then used to computationally generate synthetic 3D references for supervised 3D classification (right; colored reconstructions; protofilament number and helical `start' numbers are labeled). (b) Resulting distribution from supervised 3D classification and 3D class `smoothing' of the undecorated microtubule dataset using the references in (a). (c) Lumenal view of a final MiCSPARC refinement of a single undecorated protofilament after symmetry expansion and α/β-tubulin register correction. Obvious differences in α-tubulin versus β-tubulin S9–S10 loops are indicated. The map was post-processed using EMReady (He et al., 2023  ). (d) GDP in β-tubulin (stick representation) in the corresponding density (transparent surface representation). (e) Mg-GTP in α-tubulin (stick representation) in the corresponding density (transparent surface representation). (f) Seam view of the seam-corrected, 13-protofilament and 3-start microtubule reconstruction obtained using MiCSPARC. The location of the seam is indicated by an arrow. (g) Zoomed-in and rotated view of the seam of the microtubule reconstruction in (f). Differences in α-tubulin versus β-tubulin S9–S10 loops across the seam are indicated. |
![[Figure 3]](zi5010fig3.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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