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Figure 1
The structures of the Q108K:K40L:T51V:T53C:R58W:Y19W:T29L (cyan C atoms, λmax = 591 nm, PDB entry 4efg) and Q108K:K40L:T51V:T53C:R58W:Y19W:T29L:Q4R (green C atoms, λmax = 622 nm, PDB entry 4eej) mutants overlaid. Mutation of Gln4 leads to loss of the ordered water molecule that helps stabilize the cis-isomer, resulting in the retinylidene 15-trans conformation. All other atoms are colored by type here and in all subsequent figures (N, blue; O, red; S, yellow).

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
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