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![[Figure 3]](myl5001fig3mag.jpg)
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Figure 3
(a) Structure of the retinal-binding pocket in the blue-tinted M1 crystal (grown in the dark, 1.40 Å resolution). The imine nitrogen is surrounded by hydrophobic interactions consistent with its lower pKa of 3.3. Protein residues are colored by atom type, with C atoms in green. The retinal chromophore is cyan. (b) UV exposure of the M1 crystals give the structure of the dark blue crystal with the 15-cis iminium (1.26 Å resolution). The positive charge on the iminium is stabilized by the NH–π interaction with Trp106 [colors as in (a)]. (c) Visible light exposure of M1 crystals, resulting in nearly colorless crystals, gives the dehydrated 15-trans structure (2.11 Å resolution). (d) Overlay of the two low (cyan), and even lower (green), pKa 15-trans structures. |
![[Figure 3]](myl5001fig3.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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