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![[Figure 2]](raj5002fig2mag.jpg)
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Figure 2
Protein expression and purification DMTA workflow. The schematic illustrates the iterative design–make–test–analyze (DMTA) cycle used to optimize protein constructs for structural biology. Key upstream steps include construct design and cloning, followed by high-throughput expression screening across multiple host systems and purification development using size-exclusion chromatography (SEC), multi-angle light scattering (MALS), dynamic light scattering (DLS), ligand-binding and activity assays. Downstream activities encompass sample preparation for structural studies, crystallization screening, cryo-EM grid preparation, data collection, and computational analysis and refinement. Collectively, these integrated stages enable rapid optimization, rigorous characterization and structural determination of protein targets, supporting mechanistic insight and downstream drug-discovery efforts. |
![[Figure 2]](raj5002fig2.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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