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![[Figure 1]](vo5023fig1mag.jpg)
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Figure 1
Assessment of A2AR with different fusion tags inserted into ICL3 from small-scale affinity pull-down experiments. (a) SDS–PAGE and (b) Western blot analysis of solubilized (S) and affinity pull-down elution (E) samples of A2AR fusion constructs (numbers correspond to the fusion constructs listed in Supplementary Table S1). M, molecular-weight standards in kDa; C, Western blot anti-FLAG control; NI, non-infected cell control; F, A2AR-bRIL fusion control. (c) fSEC analysis of A2AR with two β-lactamase fusions (Fusion 5, β-lactamaseBli, and Fusion 6, β-lactamaseBan) inserted into ICL3, with A2AR-bRIL fusion control [F (bRIL)] and molecular-weight standards (Std), at 670, 158, 44, 17 and 1.35 kDa. fSEC readings were collected with an excitation wavelength of 488 nm and an emission wavelength of 512 nm; the molecular-weight standards readings (Std) are from the A280 signal. |
![[Figure 1]](vo5023fig1.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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