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Figure 2
Purification of a cross-linked hMDH2–hCS complex. (a) Size-exclusion chromatography of the cross-linked complex relative to the elution volumes of standard proteins. (b) Gel of the main fractions across the peak in (a). (c) NAD+-reduction activity of the cross-linked complex (hMDH2xhCS; red) relative to a 1:1 mixture of hMDH2 and hCS (orange) and hMDH2 alone (purple).

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
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