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Figure 3
Small-angle X-ray data for the hMDH2–hCS complex. (a) The log(I) versus q plot of the trapped hMDH2–hCS complex. (b) Comparison of hCS data in Kratky form from Fig. 2[link] (blue/cyan) to the complex showing the differences in shape. (c) FoXS fitting of the AlphaFold3 model of the MDH–CS hexamer (green, χ2 = 2.3) compared with the final best-fitting model derived from LightDock fitting (purple, χ2 = 0.85). (d) Electron density of the best-fitting MDH–CS hexamer derived from DENSS aligned with the best-fitting hexamer model from (c). The mean real-space correlation was 0.85 ± 0.04 and the Fourier shell correlation resolution was 55.6 ± 7.3 Å.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
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