forthcoming articles

We report a room-temperature serial synchrotron X-ray (SSX) crystallography structure analysis of the Nitratidesulfovibrio vulgaris formate dehydrogenase AB (NvFdhAB), an enzyme that reduces CO₂ to formate.

Replacement soaking, also known as cross-soaking, is a compelling method for solving complex structures in cases where traditional soaking and co-crystallization approaches fail. This method is demonstrated with human tankyrase 2 (TNKS2), and new crystal structures of complexes with nanomolar inhibitors and an analogue of the substrate NAD⁺ are reported.

In biological systems, hydrogels often arise through liquid-liquid phase separation (LLPS), where biomolecular condensates can age into gel-like states. SAXS is a key technique to probe the molecular organization of these samples. This study introduces an in-vacuum Gel-Cell for SAXS-based structural analysis of a biologically derived hydrogel formed by the thermosensory prion-like domain of EARLY FLOWERING 3 (ELF3) and presents a three-component model to interpret these obtained data.

In this work, Brucella melitensis Bacterioferritin (BmBfr) oligomerization was studied using X-ray crystallography, size exclusion chromatography and thermal shift analysis. Those techniques allowed the characterization of both apo- and holo-BmBfr, highlighting heme and pH impact on the oligomerization mechanism.

We present a fully automated, unattended cryo-EM pipeline that serves as an on-the-fly diagnostic tool, enabling rapid data quality assessment and informed decision-making to maximize data-collection efficiency.

A series of 36 structures of the Neurospora crassa LPMO NcAA9D were refined against X-ray crystal datasets collected with increasing dose from a single crystal to provide insights into dose-dependent structural changes at the active site of the enzyme. The findings underscore the importance of minimizing accumulated dose during cryo-X-ray crystallography studies of lytic polysaccharide monooxygenases to prevent misinterpretation of radiation damage-related electron density features.

LowDoseWizard is a guided SerialEM workflow for rapid and standardized setup of low-dose cryo-TEM imaging conditions for single particle analysis and cryo-electron tomography.

wwPDB has extended PDB IDs to 12 characters with a prefix "pdb_" followed by 8 alphanumeric characters in lower case (e.g., pdb_1000axyz). A Beta version of the PDB Archive (files-beta.wwpdb.org), with extended PDB IDs and an improved directory structure is now available to help communities adopt extended PDB IDs and PDBx/mmCIF format.

The 2-amino-pyridine moiety of KPT-7523 binds PAK4 and NAMPT, enabling a versatile design for multi-target cancer therapy.

The crystal structure of Streptomyces avermitilis endo-β-1,6-galactanase Sa16Gal30A belonging to GH30 subfamily 5 reveals the first structural framework for endo-β-1,6-galactanase. The β-1,6-galactobiose-bound complex identifies the catalytic subsites and a distal secondary sugar-binding site, providing insight into β-1,6-galactan recognition.

The open and closed lid conformational states of class C sortase (SrtC), which catalyzes the polymerization of endocarditis and biofilm-associated pili (Ebp) in Enterococcus faecalis, were captured in a single crystal. Communication between the flexible lid and the dynamic active-site (β7–β8) loop, along with their synchronized motions, regulates access to the active site for primary and secondary substrate binding.

SURFER performs automated segmentation of contextual membrane and membrane-mimic density in cryo-EM maps to enable robust separation of macromolecular signal from surrounding detergent or lipid-membrane features. It is conveniently distributed as a plugin for UCSF ChimeraX, allowing interactive application within standard map-visualization workflows.

Small but measurable changes in the coordination geometry of a lytic polysaccharide monooxygenase copper active site are revealed by X-ray crystallography, providing structural insight into their catalytic flexibility.