forthcoming articles

QwenCryoMarker is a universal post-processing framework that removes contamination-induced false positives from any cryo-EM particle picker. Built on the Qwen Image Edit vision-language model, it generates high-precision masks of carbon, ice and impurities, and then filters raw particle coordinates with negligible loss of true particles. Validated on five CryoPPP datasets with four representative particle pickers, QwenCryoMarker consistently improves precision and F₁-score, and outperforms the state-of-the-art contamination segmentation tool by 14.2% in mean intersection over union. It is plug-and-play, dataset-independent and ready for integration into mainstream cryo-EM workflows.

Ultrahigh-resolution crystallography combined with in crystallo Co K-edge XANES shows that cobalamin retains octahedral cobalt coordination in both oxidized and reduced states. These results rule out radiation-induced Co–CN cleavage as the cause of the missing axial ligand in intrinsic factor-bound cobalamin.

Conformational dynamics in the melibiose/Na⁺ cotransporter MelB significantly influence the binding of the primary sugar substrate but have minimal effect on the co-substrate Na⁺. Na⁺ acts as an allosteric activator, increasing sugar affinity by stabilizing the inner barrier and constraining conformational flexibility.