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organic compounds\(\def\hfill{\hskip 5em}\def\hfil{\hskip 3em}\def\eqno#1{\hfil {#1}}\)

Journal logoCRYSTALLOGRAPHIC
COMMUNICATIONS
ISSN: 2056-9890
Volume 66| Part 9| September 2010| Page o2339
https://doi.org/10.1107/S1600536810032186

N-(4-Meth­­oxy­phen­yl)-N′-(5-nitro-1,3-thia­zol-2-yl)urea

Alan J. Lough,a* Justin W. Hicks,b John F. Valliant,c Alan A. Wilsonb and Neil Vasdevb

aDepartment of Chemistry, University of Toronto, 80 St George Street, Toronto, Ontario, Canada M5S 3H6, bPET Centre, Centre for Addiction and Mental Health and Department of Psychiatry, University of Toronto, 250 College Street, Toronto, Ontario, Canada M5T 1R8, and cDepartments of Chemistry and Chemical Biology, and Medical Physics and Applied Radiation Sciences, McMaster University, 1280 Main Street West, Hamilton, Ontario, Canada L8S 4K1
*Correspondence e-mail: alough@chem.utoronto.ca

(Received 10 August 2010; accepted 10 August 2010; online 18 August 2010)

The title compound, C11H10N4O4S, is a derivative of N-(4-meth­oxy­benz­yl)-N′-(5-nitro-1,3-thia­zol-2-yl)urea (AR-A014418), a known glycogen synthase kinase 3β (GSK-3β) inhibitor. All non-H atoms in the mol­ecule are essentially coplanar, with an r.m.s. deviation of 0.045 Å and a maximum deviation of 0.115 (2) Å for the carbonyl O atom. In the crystal structure, mol­ecules are linked via N—H⋯O hydrogen bonds into one-dimensional chains along [101].

Related literature

For background information on the preparation and activity of AR-A014418, see: Bhat et al. (2003[Bhat, R., Xue, Y. F., Berg, S., Hellberg, S., Ormo, M., Nilsson, Y., Radesater, A. C., Jerning, E., Markgren, P. O., Borgegard, T., Nylof, M., Gimenez-Cassina, A., Hernandez, F., Lucas, J. J., Diaz-Nido, J. & Avila, J. (2003). J. Biol. Chem. 278, 45937-45945.]); Inestrosa et al. (2006[Inestrosa, N. C., Farías, G. & Colombres, M. (2006). Glycogen Synthase Kinase 3 (GSK-3) and Its Inhibitors: Drug Discovery and Development, edited by A. Martinez, A. Castro & M. Medina, pp. 25-43. New Jersey: John Wiley & Sons Inc.]). For the radiolabelling procedure of AR-A014418 with carbon-11, see: Vasdev et al. (2005[Vasdev, N., Garcia, A., Stableford, W. T., Young, A. B., Meyer, J. H., Houle, S. & Wilson, A. A. (2005). Bioorg. Med. Chem. Lett. 15, 5270-5273.]). For the crystal structure of AR-A014418, see: Vasdev et al. (2007[Vasdev, N., Wilson, A. A., Houle, S. & Lough, A. J. (2007). Acta Cryst. E63, o1653-o1655.]).

[Scheme 1]

Experimental

Crystal data

  • C11H10N4O4S

  • Mr = 294.29

  • Monoclinic, P 21 /n

  • a = 6.8740 (3) Å

  • b = 12.5840 (7) Å

  • c = 14.6861 (5) Å

  • β = 101.622 (3)°

  • V = 1244.34 (10) Å3

  • Z = 4

  • Mo Kα radiation

  • μ = 0.28 mm−1

  • T = 150 K

  • 0.28 × 0.24 × 0.22 mm
Data collection

  • Nonius KappaCCD diffractometer

  • Absorption correction: multi-scan (SORTAV; Blessing, 1995[Blessing, R. H. (1995). Acta Cryst. A51, 33-38.]) Tmin = 0.718, Tmax = 0.948

  • 8106 measured reflections

  • 2825 independent reflections

  • 1979 reflections with I > 2σ(I)

  • Rint = 0.053
Refinement

  • R[F2 > 2σ(F2)] = 0.050

  • wR(F2) = 0.145

  • S = 1.07

  • 2825 reflections

  • 190 parameters

  • H atoms treated by a mixture of independent and constrained refinement

  • Δρmax = 0.38 e Å−3

  • Δρmin = −0.51 e Å−3

Table 1
Hydrogen-bond geometry (Å, °)

D—H⋯A D—H H⋯A D⋯A D—H⋯A
N2—H2N⋯O4i 0.86 (3) 1.97 (3) 2.817 (3) 168 (3)
N3—H3N⋯O3i 0.87 (3) 2.30 (3) 3.168 (2) 174 (2)
Symmetry code: (i) [x+{\script{1\over 2}}, -y+{\script{3\over 2}}, z+{\script{1\over 2}}].

Data collection: COLLECT (Nonius, 2002[Nonius (2002). COLLECT. Nonius BV, Delft, The Netherlands.]); cell refinement: DENZO-SMN (Otwinowski & Minor, 1997[Otwinowski, Z. & Minor, W. (1997). Methods in Enzymology, Vol. 276, Macromolecular Crystallography, Part A, edited by C. W. Carter Jr & R. M. Sweet, pp. 307-326. New York: Academic Press.]); data reduction: DENZO-SMN; program(s) used to solve structure: SIR92 (Altomare et al., 1994[Altomare, A., Cascarano, G., Giacovazzo, C., Guagliardi, A., Burla, M. C., Polidori, G. & Camalli, M. (1994). J. Appl. Cryst. 27, 435.]); program(s) used to refine structure: SHELXL97 (Sheldrick, 2008[Sheldrick, G. M. (2008). Acta Cryst. A64, 112-122.]); molecular graphics: PLATON (Spek, 2009[Spek, A. L. (2009). Acta Cryst. D65, 148-155.]); software used to prepare material for publication: SHELXTL (Sheldrick, 2008[Sheldrick, G. M. (2008). Acta Cryst. A64, 112-122.]).

Supporting information

Crystal structure: contains datablocks I, global. DOI: https://doi.org/10.1107/S1600536810032186/su2205sup1.cif

Structure factors: contains datablock I. DOI: https://doi.org/10.1107/S1600536810032186/su2205Isup2.hkl

checkCIF report


Comment top

N-(4-methoxybenzyl)-N'--(5-nitro-1,3-thiazol-2-yl)urea (AR-A014418, Bhat et al., 2003) is a selective glycogen synthase kinase-3β (GSK-3β) inhibitor (Inestrosa et al., 2006). Our initial work on AR-A014418 was to radiolabel this compound with carbon-11 at the methoxy position for positron emission tomography (PET) imaging of brain pathologies (Vasdev et al., 2005). To our surprise, [11C]-AR-A014418 had insignificant brain uptake in rodents despite literature precedence (Bhat et al., 2003). To further understand the role of AR-A014418 and GSK-3β, a single-crystal X-ray structure of AR-A014418 was obtained (Vasdev et al., 2007) and overlaid with the structural determination of the co-crystal of GSK-3β and AR-A014418 (Bhat et al., 2003). For that structure, the benyzl ring was bent out of the binding pocket of the kinase. We now endeavour to explore the binding affinity of an analogous molecule which has reduced flexibility (i.e. the benzyl group is replaced with a phenyl group). Biological assays are underway to determine whether the increased rigidity decreases the binding affinity for GSK-3β. Data from these biological studies as well as the crystal structure determinations will assist in designing future ligands for imaging GSK-3β with PET.

The molecular structure of the title compound is shown in Fig. 1. All non-hydrogen atoms in the molecule are essentially co-planar with a r.m.s. deviation of 0.045 Å and a maximum deviation of 0.115 (2)Å for atom O1.

In the crystal symmetry related molecules are linked via N—H···O hydrogen bonds, to form one-dimensional chains propagating along [101] (Table 1, Fig. 2).

Related literature top

For background information on the preparation and activity of AR-A014418, see: Bhat et al. (2003); Inestrosa et al. (2006). For the radiolabelling procedure of AR-A014418 with carbon-11, see: Vasdev et al. (2005). For the crystal structure of AR-A014418, see: Vasdev et al. (2007).

Experimental top

The title compound, C11H10N4O4S, was obtained by heating equimolar amounts of 2-amino-5-nitrothiazole (0.65 mmol) and 4-methoxyphenyl isocyanate (0.65 mmol) in dry N,N-dimethyl formamide (5 ml) in a Biotage Initiator Microwave for 1 h at 403 K under nitrogen. Upon cooling, the reaction mixture was partitioned between ethyl acetate and water and the aqueous layer was further extracted with more ethyl acetate (15 ml). The combined organic layers were washed with brine (3 × 30 ml), dried (MgSO4), filtered, and concentrated prior to purification by silica chromatography (Biotage Isolera Flash system, 98% dichloromethane and 2% methanol). C11H10N4O4S was obtained as a red solid in 39% yield (not optimized). X-ray quality crystals were obtained by slow evaporation of a solution of the title compound in ethyl acetate/hexane (50/50) containing 5% ethanol. 1H NMR (DMSO d6, 400 MHz) δ 11.75 (s, 1 H, NH), 9.28 (s, 1H, NH), 8.53 (s, 1H, thiazole), 7.41 (d, J = 8.9 Hz, 2H, Ar), 6.91 (d, J = 8.9 Hz, 2H, Ar), 3.73 (s, 3H, CH3). HRMS (ESI) m/z calcd for C11H11N4O4S, 295.0495; found 295.0483 (M++H), m.p. = 454–456 K.

Refinement top

H atoms bonded to C atoms were placed in calculated positions with C—H = 0.95Å or 0.98Å for methyl groups and included in the refinement with Uiso(H) = 1.2Ueq(C) or 1.2Ueq(Cmethyl). H atoms bonded to N atoms were refined independently with isotropic displacement parameters.

Structure description top

N-(4-methoxybenzyl)-N'--(5-nitro-1,3-thiazol-2-yl)urea (AR-A014418, Bhat et al., 2003) is a selective glycogen synthase kinase-3β (GSK-3β) inhibitor (Inestrosa et al., 2006). Our initial work on AR-A014418 was to radiolabel this compound with carbon-11 at the methoxy position for positron emission tomography (PET) imaging of brain pathologies (Vasdev et al., 2005). To our surprise, [11C]-AR-A014418 had insignificant brain uptake in rodents despite literature precedence (Bhat et al., 2003). To further understand the role of AR-A014418 and GSK-3β, a single-crystal X-ray structure of AR-A014418 was obtained (Vasdev et al., 2007) and overlaid with the structural determination of the co-crystal of GSK-3β and AR-A014418 (Bhat et al., 2003). For that structure, the benyzl ring was bent out of the binding pocket of the kinase. We now endeavour to explore the binding affinity of an analogous molecule which has reduced flexibility (i.e. the benzyl group is replaced with a phenyl group). Biological assays are underway to determine whether the increased rigidity decreases the binding affinity for GSK-3β. Data from these biological studies as well as the crystal structure determinations will assist in designing future ligands for imaging GSK-3β with PET.

The molecular structure of the title compound is shown in Fig. 1. All non-hydrogen atoms in the molecule are essentially co-planar with a r.m.s. deviation of 0.045 Å and a maximum deviation of 0.115 (2)Å for atom O1.

In the crystal symmetry related molecules are linked via N—H···O hydrogen bonds, to form one-dimensional chains propagating along [101] (Table 1, Fig. 2).

For background information on the preparation and activity of AR-A014418, see: Bhat et al. (2003); Inestrosa et al. (2006). For the radiolabelling procedure of AR-A014418 with carbon-11, see: Vasdev et al. (2005). For the crystal structure of AR-A014418, see: Vasdev et al. (2007).

Computing details top

Data collection: COLLECT (Nonius, 2002); cell refinement: DENZO-SMN (Otwinowski & Minor, 1997); data reduction: DENZO-SMN (Otwinowski & Minor, 1997); program(s) used to solve structure: SIR92 (Altomare et al., 1994); program(s) used to refine structure: SHELXL97 (Sheldrick, 2008); molecular graphics: PLATON (Spek, 2009); software used to prepare material for publication: SHELXTL (Version 6.1; Sheldrick, 2008).

Figures top
[Figure 1] Fig. 1. The molecular structure of the title compound with probability ellipsoids drawn at the 30% level.
[Figure 2] Fig. 2. Part of the crystal structure of the title compound with hydrogen bonds drawn as dashed lines.
i>N-(4-Methoxyphenyl)-N'-(5-nitro-1,3-thiazol-2-yl)urea top
Crystal data top
C11H10N4O4SF(000) = 608
Mr = 294.29Dx = 1.571 Mg m−3
Monoclinic, P21/nMelting point: 454 K
Hall symbol: -P 2ynMo Kα radiation, λ = 0.71073 Å
a = 6.8740 (3) ÅCell parameters from 3592 reflections
b = 12.5840 (7) Åθ = 2.6–27.5°
c = 14.6861 (5) ŵ = 0.28 mm−1
β = 101.622 (3)°T = 150 K
V = 1244.34 (10) Å3Block, red
Z = 40.28 × 0.24 × 0.22 mm
Data collection top
Nonius KappaCCD
diffractometer		2825 independent reflections
Radiation source: fine-focus sealed tube1979 reflections with I > 2σ(I)
Graphite monochromatorRint = 0.053
Detector resolution: 9 pixels mm-1θmax = 27.5°, θmin = 2.8°
φ scans and ω scans with κ offsetsh = −8→8
Absorption correction: multi-scan
(SORTAV; Blessing, 1995)		k = −14→16
Tmin = 0.718, Tmax = 0.948l = −17→19
8106 measured reflections
Refinement top
Refinement on F2Primary atom site location: structure-invariant direct methods
Least-squares matrix: fullSecondary atom site location: difference Fourier map
R[F2 > 2σ(F2)] = 0.050Hydrogen site location: inferred from neighbouring sites
wR(F2) = 0.145H atoms treated by a mixture of independent and constrained refinement
S = 1.07 w = 1/[σ2(Fo2) + (0.0755P)2 + 0.3053P]
where P = (Fo2 + 2Fc2)/3
2825 reflections(Δ/σ)max < 0.001
190 parametersΔρmax = 0.38 e Å−3
0 restraintsΔρmin = −0.51 e Å−3
Crystal data top
C11H10N4O4SV = 1244.34 (10) Å3
Mr = 294.29Z = 4
Monoclinic, P21/nMo Kα radiation
a = 6.8740 (3) ŵ = 0.28 mm−1
b = 12.5840 (7) ÅT = 150 K
c = 14.6861 (5) Å0.28 × 0.24 × 0.22 mm
β = 101.622 (3)°
Data collection top
Nonius KappaCCD
diffractometer		2825 independent reflections
Absorption correction: multi-scan
(SORTAV; Blessing, 1995)		1979 reflections with I > 2σ(I)
Tmin = 0.718, Tmax = 0.948Rint = 0.053
8106 measured reflections
Refinement top
R[F2 > 2σ(F2)] = 0.0500 restraints
wR(F2) = 0.145H atoms treated by a mixture of independent and constrained refinement
S = 1.07Δρmax = 0.38 e Å−3
2825 reflectionsΔρmin = −0.51 e Å−3
190 parameters
Special details top

Geometry. All e.s.d.'s (except the e.s.d. in the dihedral angle between two l.s. planes) are estimated using the full covariance matrix. The cell e.s.d.'s are taken into account individually in the estimation of e.s.d.'s in distances, angles and torsion angles; correlations between e.s.d.'s in cell parameters are only used when they are defined by crystal symmetry. An approximate (isotropic) treatment of cell e.s.d.'s is used for estimating e.s.d.'s involving l.s. planes.

Refinement. Refinement of F2 against ALL reflections. The weighted R-factor wR and goodness of fit S are based on F2, conventional R-factors R are based on F, with F set to zero for negative F2. The threshold expr ession of F2 > σ(F2) is used only for calculating R-factors(gt) etc. and is not relevant to the choice of reflections for refinement. R-factors based on F2 are statistically about twice as large as those based on F, and R- factors based on ALL data will be even larger.

Fractional atomic coordinates and isotropic or equivalent isotropic displacement parameters (Å2) top
xyzUiso*/Ueq
S10.12900 (8)0.68571 (5)0.41433 (3)0.0246 (2)
O10.2254 (2)0.48461 (14)0.47569 (10)0.0306 (4)
O20.5006 (3)0.00530 (13)0.62455 (11)0.0340 (4)
O3−0.0126 (3)0.97318 (14)0.33409 (11)0.0347 (4)
O40.0063 (3)0.82488 (15)0.25900 (11)0.0367 (5)
N10.2026 (3)0.79137 (16)0.57084 (12)0.0277 (5)
N20.2668 (3)0.61085 (16)0.58904 (13)0.0268 (5)
H2N0.327 (4)0.627 (2)0.6449 (19)0.042 (8)*
N30.3514 (3)0.44014 (16)0.62860 (13)0.0253 (5)
H3N0.380 (4)0.466 (2)0.6845 (19)0.037 (8)*
N40.0257 (3)0.87648 (16)0.33258 (13)0.0270 (5)
C10.0930 (3)0.82155 (19)0.41566 (14)0.0232 (5)
C20.1399 (3)0.8632 (2)0.50308 (15)0.0267 (5)
H2A0.12920.93690.51530.032*
C30.2033 (3)0.69504 (18)0.53334 (15)0.0224 (5)
C40.2790 (3)0.50759 (19)0.55815 (15)0.0243 (5)
C50.3832 (3)0.32879 (18)0.62462 (15)0.0223 (5)
C60.3583 (3)0.2717 (2)0.54193 (15)0.0245 (5)
H6A0.31530.30640.48390.029*
C70.3970 (3)0.1640 (2)0.54544 (15)0.0258 (5)
H7A0.37910.12450.48920.031*
C80.4620 (3)0.11186 (19)0.63008 (15)0.0253 (5)
C90.4842 (4)0.1688 (2)0.71214 (16)0.0281 (6)
H9A0.52650.13420.77020.034*
C100.4439 (4)0.2773 (2)0.70889 (16)0.0277 (5)
H10A0.45830.31640.76510.033*
C110.5519 (4)−0.0525 (2)0.70992 (17)0.0360 (6)
H11A0.5715−0.12760.69650.054*
H11B0.4446−0.04600.74450.054*
H11C0.6749−0.02360.74720.054*
Atomic displacement parameters (Å2) top
U11U22U33U12U13U23
S10.0269 (3)0.0260 (4)0.0198 (3)0.0001 (2)0.0024 (2)−0.0017 (2)
O10.0405 (10)0.0273 (10)0.0225 (9)0.0016 (8)0.0028 (7)−0.0024 (7)
O20.0482 (11)0.0242 (9)0.0304 (9)0.0047 (8)0.0097 (8)0.0018 (7)
O30.0418 (11)0.0279 (10)0.0335 (10)0.0046 (8)0.0057 (8)0.0080 (8)
O40.0450 (11)0.0437 (12)0.0193 (8)0.0073 (9)0.0015 (7)−0.0019 (8)
N10.0368 (12)0.0251 (11)0.0214 (10)0.0020 (9)0.0059 (8)−0.0009 (8)
N20.0352 (12)0.0240 (11)0.0205 (10)0.0011 (9)0.0040 (8)−0.0033 (9)
N30.0315 (11)0.0238 (11)0.0204 (10)0.0003 (9)0.0048 (8)−0.0014 (9)
N40.0239 (11)0.0318 (12)0.0250 (10)0.0008 (9)0.0041 (8)0.0044 (9)
C10.0226 (12)0.0249 (13)0.0223 (11)0.0005 (10)0.0049 (9)0.0036 (10)
C20.0310 (13)0.0242 (13)0.0246 (12)0.0003 (10)0.0046 (9)−0.0005 (10)
C30.0241 (12)0.0231 (13)0.0206 (11)−0.0022 (9)0.0063 (8)−0.0015 (9)
C40.0243 (12)0.0251 (13)0.0247 (12)−0.0007 (10)0.0079 (9)−0.0009 (10)
C50.0206 (11)0.0247 (13)0.0226 (11)−0.0003 (9)0.0070 (8)0.0002 (10)
C60.0233 (12)0.0285 (14)0.0219 (11)0.0006 (10)0.0051 (9)0.0007 (10)
C70.0253 (12)0.0293 (14)0.0233 (11)−0.0025 (10)0.0063 (9)−0.0030 (10)
C80.0257 (12)0.0214 (12)0.0309 (12)0.0006 (10)0.0105 (9)0.0002 (10)
C90.0310 (13)0.0314 (14)0.0231 (11)0.0006 (11)0.0087 (9)0.0044 (10)
C100.0338 (14)0.0273 (13)0.0235 (12)0.0006 (11)0.0097 (10)−0.0020 (10)
C110.0477 (16)0.0261 (14)0.0345 (14)0.0045 (12)0.0089 (11)0.0074 (11)
Geometric parameters (Å, º) top
S1—C31.723 (2)C1—C21.364 (3)
S1—C11.728 (2)C2—H2A0.9500
O1—C41.227 (3)C5—C101.385 (3)
O2—C81.373 (3)C5—C61.391 (3)
O2—C111.431 (3)C6—C71.381 (3)
O3—N41.246 (3)C6—H6A0.9500
O4—N41.245 (2)C7—C81.397 (3)
N1—C31.332 (3)C7—H7A0.9500
N1—C21.349 (3)C8—C91.384 (3)
N2—C31.356 (3)C9—C101.391 (3)
N2—C41.384 (3)C9—H9A0.9500
N2—H2N0.86 (3)C10—H10A0.9500
N3—C41.353 (3)C11—H11A0.9800
N3—C51.421 (3)C11—H11B0.9800
N3—H3N0.87 (3)C11—H11C0.9800
N4—C11.398 (3)
C3—S1—C186.27 (11)C10—C5—C6120.0 (2)
C8—O2—C11117.49 (19)C10—C5—N3116.5 (2)
C3—N1—C2109.40 (18)C6—C5—N3123.5 (2)
C3—N2—C4124.68 (19)C7—C6—C5119.0 (2)
C3—N2—H2N115.2 (19)C7—C6—H6A120.5
C4—N2—H2N118.6 (19)C5—C6—H6A120.5
C4—N3—C5128.5 (2)C6—C7—C8121.3 (2)
C4—N3—H3N117.8 (18)C6—C7—H7A119.4
C5—N3—H3N113.6 (18)C8—C7—H7A119.4
O4—N4—O3122.61 (19)O2—C8—C9124.7 (2)
O4—N4—C1117.3 (2)O2—C8—C7115.9 (2)
O3—N4—C1120.09 (19)C9—C8—C7119.4 (2)
C2—C1—N4127.3 (2)C8—C9—C10119.5 (2)
C2—C1—S1112.58 (17)C8—C9—H9A120.3
N4—C1—S1120.16 (17)C10—C9—H9A120.3
N1—C2—C1114.6 (2)C5—C10—C9120.8 (2)
N1—C2—H2A122.7C5—C10—H10A119.6
C1—C2—H2A122.7C9—C10—H10A119.6
N1—C3—N2119.29 (19)O2—C11—H11A109.5
N1—C3—S1117.15 (17)O2—C11—H11B109.5
N2—C3—S1123.53 (17)H11A—C11—H11B109.5
O1—C4—N3126.7 (2)O2—C11—H11C109.5
O1—C4—N2121.2 (2)H11A—C11—H11C109.5
N3—C4—N2112.05 (19)H11B—C11—H11C109.5
Hydrogen-bond geometry (Å, º) top
D—H···AD—HH···AD···AD—H···A
N2—H2N···O4i0.86 (3)1.97 (3)2.817 (3)168 (3)
N3—H3N···O3i0.87 (3)2.30 (3)3.168 (2)174 (2)
Symmetry code: (i) x+1/2, −y+3/2, z+1/2.

Experimental details

Crystal data
Chemical formulaC11H10N4O4S
Mr294.29
Crystal system, space groupMonoclinic, P21/n
Temperature (K)150
a, b, c (Å)6.8740 (3), 12.5840 (7), 14.6861 (5)
β (°) 101.622 (3)
V (Å3)1244.34 (10)
Z4
Radiation typeMo Kα
µ (mm−1)0.28
Crystal size (mm)0.28 × 0.24 × 0.22
Data collection
DiffractometerNonius KappaCCD
Absorption correctionMulti-scan
(SORTAV; Blessing, 1995)
Tmin, Tmax0.718, 0.948
No. of measured, independent and
observed [I > 2σ(I)] reflections		8106, 2825, 1979
Rint0.053
(sin θ/λ)max (Å−1)0.649
Refinement
R[F2 > 2σ(F2)], wR(F2), S 0.050, 0.145, 1.07
No. of reflections2825
No. of parameters190
H-atom treatmentH atoms treated by a mixture of independent and constrained refinement
Δρmax, Δρmin (e Å−3)0.38, −0.51

Computer programs: COLLECT (Nonius, 2002), DENZO-SMN (Otwinowski & Minor, 1997), SIR92 (Altomare et al., 1994), SHELXL97 (Sheldrick, 2008), PLATON (Spek, 2009), SHELXTL (Version 6.1; Sheldrick, 2008).

Hydrogen-bond geometry (Å, º) top
D—H···AD—HH···AD···AD—H···A
N2—H2N···O4i0.86 (3)1.97 (3)2.817 (3)168 (3)
N3—H3N···O3i0.87 (3)2.30 (3)3.168 (2)174 (2)
Symmetry code: (i) x+1/2, −y+3/2, z+1/2.
 

Acknowledgements

We thank Dr Sylvain Houle for allowing the CAMH PET Centre facilities to be used for this research. Funding was provided by the Ontario Ministry for Research and Innovation (Early Researcher Award to NV).

References

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ISSN: 2056-9890
Volume 66| Part 9| September 2010| Page o2339
https://doi.org/10.1107/S1600536810032186
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