research communications\(\def\hfill{\hskip 5em}\def\hfil{\hskip 3em}\def\eqno#1{\hfil {#1}}\)

Journal logoCRYSTALLOGRAPHIC
COMMUNICATIONS
ISSN: 2056-9890
Volume 74| Part 4| April 2018| Pages 445-449
https://doi.org/10.1107/S2056989018002992

Crystal structure of diaphorin methanol monosolvate isolated from Diaphorina citri Kuwayama, the insect vector of citrus greening disease

CROSSMARK_Color_square_no_text.svg
D. Marian Szebenyi,a* Irina Kriksunov,a Kevin J. Howe,b John S. Ramsey,b David G. Hall,c Michelle L. Heckb,d,e* and Stuart B. Krasnoffb*

aCornell High Energy Synchrotron Source, Cornell University, Ithaca, NY 14853, USA, bUSDA-ARS RW Holley Center for Agriculture and Health, Ithaca NY 14853, USA, cU.S. Horticultural Research Laboratory, Fort Pierce, FL 34945, USA, dBoyce Thompson Institute for Plant Research, Ithaca, NY 14853, USA, and ePlant Pathology and Plant-Microbe Biology Section, Cornell University, Ithaca, NY 14853, USA
*Correspondence e-mail: dms35@cornell.edu, michelle.cilia@ars.usda.gov, stuart.krasnoff@ars.usda.gov

Edited by H. Stoeckli-Evans, University of Neuchâtel, Switzerland (Received 7 February 2018; accepted 20 February 2018; online 2 March 2018)

The title compound C22H39NO9·CH3OH [systematic name: (S)-N-((S)-{(2S,4R,6R)-6-[(S)-2,3-di­hydroxy­prop­yl]-4-hy­droxy-5,5-di­methyl­tetra­hydro-2H-pyran-2-yl}(hy­droxy)meth­yl)-2-hy­droxy-2-[(2R,5R,6R)-2-meth­oxy-5,6-dimeth­yl-4-methyl­ene­tetra­hydro-2H-pyran-2-yl]acetamide methanol monosolvate], was isolated from the Asian citrus psyllid, Diaphorina citri Kuwayama, and crystallizes in the space group P21. `Candidatus Profftella armatura' a bacterial endosymbiont of D. citri, biosynthesizes diaphorin, which is a hybrid polyketide–nonribosomal peptide comprising two highly substituted tetra­hydro­pyran rings joined by an N-acyl aminal bridge [Nakabachi et al. (2013[Nakabachi, A., Ueoka, R., Oshima, K., Teta, R., Mangoni, A., Gurgui, M., Oldham, N. J., van Echten-Deckert, G., Okamura, K., Yamamoto, K., Inoue, H., Ohkuma, M., Hongoh, Y., Miyagishima, S. Y., Hattori, M., Piel, J. & Fukatsu, T. (2013). Curr. Biol. 23, 1478-1484.]). Curr. Biol. 23, 1478–1484]. The crystal structure of the title compound establishes the complete relative configuration of diaphorin, which agrees at all nine chiral centers with the structure of the methanol monosolvate of the di-p-bromo­benzoate derivative of pederin, a biogenically related compound whose crystal structure was reported previously [Furusaki et al. (1968[Furusaki, A., Watanabé, T., Matsumoto, T. & Yanagiya, M. (1968). Tetrahedron Lett. 9, 6301-6304.]). Tetra­hedron Lett. 9, 6301–6304]. Thus, the absolute configuration of diaphorin is proposed by analogy to that of pederin.

CCDC reference: 1824900

Similar articles

1. Chemical context

Huanglongbing (HLB), also known as citrus greening disease, which destroys the marketability of citrus fruit and eventually kills the tree, is a major threat to world citrus production (Wang et al., 2017[Wang, N., Stelinski, L. L., Pelz-Stelinski, K. S., Graham, J. H. & Zhang, Y. (2017). Phytopathology, 107, 380-387.]; Bové, 2006[Bové, J. M. (2006). J. Plant Pathol. 7-37.]). HLB is associated with plant infection by one of three fastidious bacterial species, `Candidatus Liberibacter asiaticus', `Candidatus Liberibacter americanus' or `Candidatus Liberibacter africanus'. All three bacteria are spread within a grove by psyllids – sap-sucking insects in the order Hemiptera. In North America, `Ca. L. asiaticus' is transmitted by the invasive citrus pest, the Asian citrus psyllid, Diaphorina citri Kuwayama. A complex community of vertically transmitted endosymbiotic bacteria colonizes D. citri, whether or not the psyllids are infected with `Ca. L. asiaticus' (Nakabachi et al., 2013[Nakabachi, A., Ueoka, R., Oshima, K., Teta, R., Mangoni, A., Gurgui, M., Oldham, N. J., van Echten-Deckert, G., Okamura, K., Yamamoto, K., Inoue, H., Ohkuma, M., Hongoh, Y., Miyagishima, S. Y., Hattori, M., Piel, J. & Fukatsu, T. (2013). Curr. Biol. 23, 1478-1484.]). Two of these D. citri endosymbionts, `Candidatus Profftella armatura', and `Candidatus Carsonella rudii' are localized to the bacteriome, an organ in the D. citri abdomen (Nakabachi et al., 2013[Nakabachi, A., Ueoka, R., Oshima, K., Teta, R., Mangoni, A., Gurgui, M., Oldham, N. J., van Echten-Deckert, G., Okamura, K., Yamamoto, K., Inoue, H., Ohkuma, M., Hongoh, Y., Miyagishima, S. Y., Hattori, M., Piel, J. & Fukatsu, T. (2013). Curr. Biol. 23, 1478-1484.]). While `Ca. C. rudii' is the primary endosymbiont of many psyllid species, `Ca. P. armatura' is only found in D. citri and has been detected in every D. citri population surveyed, worldwide (Nakabachi et al., 2013[Nakabachi, A., Ueoka, R., Oshima, K., Teta, R., Mangoni, A., Gurgui, M., Oldham, N. J., van Echten-Deckert, G., Okamura, K., Yamamoto, K., Inoue, H., Ohkuma, M., Hongoh, Y., Miyagishima, S. Y., Hattori, M., Piel, J. & Fukatsu, T. (2013). Curr. Biol. 23, 1478-1484.]). Approximately 15% of the `Ca. P. armatura' genome is composed of a hybrid polyketide synthase (PKS)/nonribosomal peptide synthetase (NRPS) gene and associated tailoring genes dedicated to the biosynthesis of diaphorin. Because `Ca. P. armatura' is unculturable, diaphorin is extracted directly from its D. citri host (Nakabachi et al., 2013[Nakabachi, A., Ueoka, R., Oshima, K., Teta, R., Mangoni, A., Gurgui, M., Oldham, N. J., van Echten-Deckert, G., Okamura, K., Yamamoto, K., Inoue, H., Ohkuma, M., Hongoh, Y., Miyagishima, S. Y., Hattori, M., Piel, J. & Fukatsu, T. (2013). Curr. Biol. 23, 1478-1484.]). Diaphorin is a hybrid poly­ketide–nonribosomal peptide in which two highly function­alized tetra­hydro­pyran rings are joined by an N-acyl aminal bridge. It is a tri-O-desmethyl analog of pederin, a potent cytotoxin deriving from an undetermined Pseudomonas-like endosymbiont of staphylinid beetles in the genus Paederus (Cardani et al., 1967[Cardani, C., Ghiringhelli, D., Quilico, A. & Selva, A. (1967). Tetrahedron Lett. 8, 4023-4025.]; Mosey & Floreancig, 2012[Mosey, R. A. & Floreancig, P. E. (2012). Nat. Prod. Rep. 29, 980-995.]; Cardani et al., 1965[Cardani, C., Ghiringhelli, D., Mondelli, R. & Quilico, A. (1965). Tetrahedron Lett. 6, 2537-2545.]; Furusaki et al., 1968[Furusaki, A., Watanabé, T., Matsumoto, T. & Yanagiya, M. (1968). Tetrahedron Lett. 9, 6301-6304.]; Matsumoto et al., 1968[Matsumoto, T., Yanagiya, M., Maeno, S. & Yasuda, S. (1968). Tetrahedron Lett. 9, 6297-6300.]; Piel, 2002[Piel, J. (2002). Proc. Natl Acad. Sci. USA, 99, 14002-14007.]). Nakabachi et al. (2013[Nakabachi, A., Ueoka, R., Oshima, K., Teta, R., Mangoni, A., Gurgui, M., Oldham, N. J., van Echten-Deckert, G., Okamura, K., Yamamoto, K., Inoue, H., Ohkuma, M., Hongoh, Y., Miyagishima, S. Y., Hattori, M., Piel, J. & Fukatsu, T. (2013). Curr. Biol. 23, 1478-1484.]) assigned the relative configuration of six of the nine stereogenic centers in diaphorin, but carbons 7, 10 and 17 remained unspecified. We pursued the crystal structure of diaphorin to complete the assignment of the relative configuration of the mol­ecule.

[Scheme 1]

2. Structural commentary

The title compound crystallizes in the monoclinic P21 space group and features an N-acyl aminal bridge that connects two highly substituted tetra­hydro­pyran rings adopting chair conformations (Fig. 1[link]). Ring A substitutions comprise an equatorial methyl group on C2, an axial methyl group on C3, an exo­methyl­ene group on C4, and a meth­oxy group at C6. Ring A (O1/C2–C6) has a chair conformation with puckering parameters: amplitude Q = 0.541 (4) Å, θ = 173.0 (5)°, φ = 265 (4)°. Ring B (O11/C11–C15) substitutions comprise a hydroxyl group at C13, a geminal pair of methyl groups at C14 and a 2,3 di­hydroxy­propyl group at C15. It also has a chair conformation with puckering parameters: amplitude Q = 0.559 (4) Å, θ = 8.1 (4)°, φ = 258 (3)°. The mean planes of rings A and B are inclined to each other at an angle of 80.1 (2)°. For the plane including the central amide bond, (C7/O7/C8/O8/N9/C10), the r.m.s. deviation from the plane for those atoms is 0.045 Å. This planar conformation is likely influenced by a hydrogen bond in which the amide proton H9 is the donor and O7 is the acceptor with an inter­atomic distance of 2.16 Å between the participants (Fig. 1[link], Table 1[link]). The chain from C13 through O18, viz. C13–C18/O18, is seen to be approximately planar, with an r.m.s. deviation from the plane of 0.117 Å. This conformation appears to result from crystal-packing inter­actions and probably has no biological significance. The crystal structure of the title compound assigns the three chiral centers left undetermined by Nakabachi et al. (2013[Nakabachi, A., Ueoka, R., Oshima, K., Teta, R., Mangoni, A., Gurgui, M., Oldham, N. J., van Echten-Deckert, G., Okamura, K., Yamamoto, K., Inoue, H., Ohkuma, M., Hongoh, Y., Miyagishima, S. Y., Hattori, M., Piel, J. & Fukatsu, T. (2013). Curr. Biol. 23, 1478-1484.]) as 10S*, 13R*, and 17S*, and thus provides the complete relative configuration of diaphorin. The absolute configuration, as depicted in Fig. 1[link], was inferred by analogy to that of pederin di-p-bromo­benzoate (Furusaki et al., 1968[Furusaki, A., Watanabé, T., Matsumoto, T. & Yanagiya, M. (1968). Tetrahedron Lett. 9, 6301-6304.]), which it matches at all stereogenic centers.

Table 1
Hydrogen-bond geometry (Å, °)

D—H⋯A D—H H⋯A D⋯A D—H⋯A
N9—H9⋯O7 0.88 2.16 2.594 (12) 109
O7—H7O⋯O50 0.84 1.83 2.672 (15) 178
O50—H50O⋯O18 0.84 1.85 2.651 (11) 160
O13—H13O⋯O6i 0.84 2.50 2.927 (12) 113
O17—H17O⋯O10ii 0.84 2.03 2.796 (11) 152
O18—H18O⋯O8iii 0.84 2.04 2.708 (14) 136
Symmetry codes: (i) [-x+1, y-{\script{1\over 2}}, -z+1]; (ii) [-x+1, y+{\script{1\over 2}}, -z+1]; (iii) [-x+2, y+{\script{1\over 2}}, -z+1].
[Figure 1]
Figure 1
A view of the mol­ecular structure of the title compound, with the atom labeling. Displacement ellipsoids are drawn at the 50% probability level. Hydrogen bonds are shown as thin black lines (see Table 1[link])

3. Supra­molecular features

The crystal structure was found to contain one methanol mol­ecule, forming two hydrogen bonds to diaphorin; the methanol OH acts as a proton donor to O18 and an acceptor of a proton from O7 (Fig. 1[link], Table 1[link]). The diaphorin–methanol group forms a compact, roughly planar disk; disks are packed in a herringbone fashion as illustrated in Fig. 2[link]. Inter­molecular contacts between symmetry-related diaphorin mol­ecules include probable hydrogen bonds between O13 (donor) and O6′ (acceptor); O17 (donor) and O10′ (acceptor); and O18 (donor) and O8′ (acceptor), as shown in Fig. 2[link], see also Table 1[link]. The combination of these inter­molecular inter­actions leads to the formation of slabs lying parallel to the ab plane.

[Figure 2]
Figure 2
A view normal to the ab plane of the crystal packing of the title compound. The methanol solvent mol­ecules are shown in green and the hydrogen bonds as thin red lines (see Table 1[link]).

4. Database survey

A search of the Cambridge Structural Database (CSD, Version 5.38, update May 2017; Groom et al., 2016[Groom, C. R., Bruno, I. J., Lightfoot, M. P. & Ward, S. C. (2016). Acta Cryst. B72, 171-179.]) for related structures gave two hits. They are pederin di-p-bromo­benzoate methanol monosolvate (CSD refcode PEDERB; CCDC No. 1229933; Furusaki et al., 1968[Furusaki, A., Watanabé, T., Matsumoto, T. & Yanagiya, M. (1968). Tetrahedron Lett. 9, 6301-6304.]), for which no atomic coordinates are available, and pederin di-p-bromo­benzoate ethanol monosolvate (CSD refcode BPEDER; CCDC No. 1114946; Corradi et al., 1971[Corradi, A. B., Mangia, A., Nadelli, M. & Pelizzi, G. (1971). Gazz. Chim. Ital. 101, 591.]). They both have the same skeleton as diaphorin, except for the addition of the two p-bromo­benzoate substituents. The structure of diaphorin can be matched to that of pederin by rotations about the following single bonds: C7—C8, N9—C10, C10—C11, and bonds in the C15–O18 moiety.

5. Isolation and crystallization

Diaphorin was isolated using a liquid–liquid extraction scheme with semi-preparative HPLC with modifications from the published method (Nakabachi et al., 2013[Nakabachi, A., Ueoka, R., Oshima, K., Teta, R., Mangoni, A., Gurgui, M., Oldham, N. J., van Echten-Deckert, G., Okamura, K., Yamamoto, K., Inoue, H., Ohkuma, M., Hongoh, Y., Miyagishima, S. Y., Hattori, M., Piel, J. & Fukatsu, T. (2013). Curr. Biol. 23, 1478-1484.]). A batch of ca 3000 D. citri was reared on Citrus macrophylla (not infected by `Ca. L. asiaticus') at the US Horticultural Research Laboratory, Fort Pierce, FL 34945, USA. Insects were allocated to 2 ml microcentrifuge tubes, then flash frozen in liquid N2 and cryoground for 3.5 min at 30 Hz using 3 × 3.2 mm metal beads per tube in a ball mill apparatus (Retsch Mixer Miller MM-400). Ground insects in each tube were then extracted three times in MeOH for 45 min at 298 K. After agitation, the tubes were centrifuged for 2 min at 16,000 g, and the supernatants were pooled, filtered through two layers of Whatman #1 paper and dried in vacuo. The residue was taken up in 90% MeOH and partitioned against cyclo­hexane. The methano­lic phase was then fractionated by repetitive semi-preparative reversed phase HPLC using a Thermo Fluophase® column (250 × 10 mm ID, 5 µm particle), eluted at 4 ml min−1 with 20% MeCN, and 1 ml fractions were collected. Following detection by UV absorption at 215 nm, selected fractions were monitored for the presence of diaphorin by syringe pump infusion (5 µL min−1) into a Waters-Micromass ZQ single quadrupole mass spectrometer (scan range: m/z 50–1500 in 1 sec with cone and capillary voltages of 25 and 3500 V, respectively). Fractions showing the pseudomolecular ion of diaphorin (M + Na+ at m/z 484) were recombined and dried in vacuo to afford ca 4.0 mg of diaphorin. Crystals of the title compound were obtained by slow evaporation from MeOH. A single crystal measuring approximately 0.01 × 0.02 × 0.20 mm was harvested using a needle dipped in a drop of oil for adhesion (type A immersion oil, Hampton Research Corp.) and mounted in a small nylon loop (Hampton). The identity and purity of diaphorin was confirmed by comparing 1H NMR data acquired using the sample that afforded crystals with published data (Nakabachi et al., 2013[Nakabachi, A., Ueoka, R., Oshima, K., Teta, R., Mangoni, A., Gurgui, M., Oldham, N. J., van Echten-Deckert, G., Okamura, K., Yamamoto, K., Inoue, H., Ohkuma, M., Hongoh, Y., Miyagishima, S. Y., Hattori, M., Piel, J. & Fukatsu, T. (2013). Curr. Biol. 23, 1478-1484.]). Further confirmation was obtained by HPLC with detection by high resolution electrospray mass spectrometry (HRESIMS). Retention time (tR) and accurate mass estimates were compared with those of authentic diaphorin using a Waters Acquity UPLC system with a Waters C18 BEH column (2.1 × 50 mm; 1.7 µm), eluted at 0.3 ml min−1 using a gradient formed from 0.1% formic acid (A) and aceto­nitrile (B) with 0.1% formic acid (90% A 0–1 min, 14 min linear ramp to 80% A, followed by a 1 min ramp to 10% A, a 2 min hold, and a ramp back to 90% A in 1 min). Spectra were acquired on a Waters Xevo G-2 QTOF mass spectrometer operated in positive ion mode scanning the mass range from m/z 50 to 1200 in 0.1 sec with capillary and cone voltages set at 3.5 V and 25 k V, respectively. The spectrometer was calibrated in the range m/z 50–1200 using sodium formate. Spectra were calibrated in real-time using the M + H+ of co-infused leucine encephalin (m/z 556.2771) as the reference and were further processed by centering using the proprietary `automatic peak detection' tool supplied with Waters MassLynx® 4.1 software.

1H NMR (AVIII HD 500, Bruker BioSpin, Rheinstetten Germany, 500 MHz, CD3OD), referenced to the center of the residual CHD2OD pentet at δH 3.31. δH (p.p.m.) 5.60 (d, J = 7.9 Hz, 1H, H-10), 4.80 (t, J = 2.2 Hz, 1H, H-4-CHa), 4.64 (t, J = 2.2 Hz, 1H, H-4-CHb), 4.26 (s, 1H, H-7), 3.882 (m, 2H, H-2), 3.880 (m, 2H, H-11), 3.76 (qd, J = 4.0, 6.3 Hz, 1H, H-17), 3.61 (dd, J = 4.4, 10.3 Hz, 1H, H-13), 3.49 (dd, J = 4.1, 11.2 Hz, 1H, H-18a), 3.40 (m, 2H, 15, H-18b), 3.25 (s, 3H, H-6-OCH3), 2.50 (dt, J = 2.2, 14.3 Hz, 1H, H-5 ax), 2.31 (d, J = 14.3 Hz, 1H, H-5 eq), 2.20 (qd, J = 2.5, 7.0 Hz, 1H, H-3), 2.04 (ddd, J = 3.3, 4.4, 13.5 Hz, 1H, H-12eq), 1.76 (ddd, J = 5.9, 10.3, 13.5 Hz, 1H, H-12ax), 1.67 (t, J = 6.2 Hz, 2H, H-16), 1.17 (d, J = 6.6 Hz, 3H, H-2–CH3), 0.99 (d, J = 7.0 Hz, 3H, H-3–CH3), 0.95 (s, 3H, H-14–CH3 eq), 0.88 (s, 3H, H-14–CH3 ax). HRESIMS m/z 484.2521 (calculated for C22H39NO9Na, 484.2517); tR = 8.61 min.

6. Refinement

Crystal data, data collection and structure refinement details are summarized in Table 2[link]. The hydrogen atoms were fixed geometrically (O—H = 0.84 Å, N—H = 0.86 Å, C—H = 0.98–0.10 Å) and allowed to ride on their parent atoms with Uiso(H) = 1.5Ueq(C-methyl, O-hydrox­yl) and 1.2Ueq(N, C) for other H atoms.

Table 2
Experimental details

Crystal data
Chemical formula C22H39NO9·CH4O
Mr 493.58
Crystal system, space group Monoclinic, P21
Temperature (K) 100
a, b, c (Å) 7.40 (5), 12.87 (5), 13.92 (5)
β (°) 101.9 (5)
V (Å3) 1297 (11)
Z 2
Radiation type Synchrotron, λ = 0.9768 Å
μ (mm−1) 0.10
Crystal size (mm) 0.20 × 0.02 × 0.01
 
Data collection
Diffractometer Single-axis goniometer with Dectris Pilatus 6M detector
Absorption correction Empirical (using intensity measurements) XDS (Kabsch, 2010[Kabsch, W. (2010). Acta Cryst. D66, 125-132.]), determined correction factors as a function of position on detector surface and frame number
No. of measured, independent and observed [I > 2σ(I)] reflections 7818, 2610, 2594
Rint 0.054
θmax (°) 31.0
(sin θ/λ)max (Å−1) 0.527
 
Refinement
R[F2 > 2σ(F2)], wR(F2), S 0.044, 0.110, 1.06
No. of reflections 2610
No. of parameters 309
No. of restraints 4
H-atom treatment H-atom parameters constrained
Δρmax, Δρmin (e Å−3) 0.32, −0.20
Computer programs: ADX (Szebenyi et al., 1997[Szebenyi, D. M. E., Arvai, A., Ealick, S., LaIuppa, J. M. & Nielsen, C. (1997). J. Synchrotron Rad. 4, 128-135.]), HKL-2000 (Otwinowski & Minor, 1997[Otwinowski, Z. & Minor, W. (1997). Methods in Enzymology, Vol. 276, Macromolecular Crystallography, Part A, edited by C. W. Carter Jr & R. M. Sweet, pp. 307-326. New York: Academic Press.]), XDS (Kabsch, 2010[Kabsch, W. (2010). Acta Cryst. D66, 125-132.]), SnB (Weeks & Miller, 1999a[Weeks, C. M. & Miller, R. (1999a). J. Appl. Cryst. 32, 120-124.],b[Weeks, C. M. & Miller, R. (1999b). Acta Cryst. D55, 492-500.]), COOT (Emsley et al., 2010[Emsley, P., Lohkamp, B., Scott, W. G. & Cowtan, K. (2010). Acta Cryst. D66, 486-501.]), ORTEPIII (Burnett & Johnson, 1996[Burnett, M. N. & Johnson, C. K. (1996). ORTEPIII. Report ORNL-6895. Oak Ridge National Laboratory, Tennessee, USA.]), SHELXL2016 (Sheldrick, 2015[Sheldrick, G. M. (2015). Acta Cryst. C71, 3-8.]), PLATON (Spek, 2009[Spek, A. L. (2009). Acta Cryst. D65, 148-155.]) and publCIF (Westrip, 2010[Westrip, S. P. (2010). J. Appl. Cryst. 43, 920-925.]).

The absolute structure of the mol­ecule in the crystal could not be determined by resonant scattering. It was assigned by analogy to that of pederin di-p-bromo­benzoate methanol monosolvate (Furusaki et al., 1968[Furusaki, A., Watanabé, T., Matsumoto, T. & Yanagiya, M. (1968). Tetrahedron Lett. 9, 6301-6304.]), for which no atomic coordinates are available, and pederin di-p-bromo­benzoate ethanol monosolvate (Corradi et al., 1971[Corradi, A. B., Mangia, A., Nadelli, M. & Pelizzi, G. (1971). Gazz. Chim. Ital. 101, 591.]), for which the absolute configurations were determined by resonant scattering.

X-ray crystallographic data were collected at the Cornell High Energy Synchrotron Source (Ithaca, NY, 14853, USA). The synchrotron beamline available to us (CHESS F1) is normally used for macromolecular data collection. It is a fixed-wavelength line and it is not possible (due to inter­ference with equipment including the crystal-mounting robot) to move the area detector (Pilatus 6M) close enough to the sample to record data beyond 0.95 Å (in the corners; only to 1.15 Å at the edges). This explains the lack of high-resolution data, and the large s.u.'s on the cell dimensions, which may also be related to the use of the program XDS, which is typically used for macromolecular data reduction, for refinement of these and other experimental parameters.

Supporting information

CCDC reference: 1824900

Crystal structure: contains datablocks I, Global. DOI: https://doi.org/10.1107/S2056989018002992/su5424sup1.cif

Structure factors: contains datablock I. DOI: https://doi.org/10.1107/S2056989018002992/su5424Isup2.hkl

Supporting information file. DOI: https://doi.org/10.1107/S2056989018002992/su5424Isup3.cml

checkCIF report


Computing details top

Data collection: ADX (Szebenyi et al., 1997); cell refinement: XDS (Kabsch, 2010); data reduction: HKL-2000 (Otwinowski & Minor, 1997) and XDS (Kabsch, 2010); program(s) used to solve structure: SnB (Weeks & Miller, 1999a,b) and COOT (Emsley et al., 2010); program(s) used to refine structure: SHELXL2016 (Sheldrick, 2015); molecular graphics: ORTEPIII (Burnett & Johnson, 1996); software used to prepare material for publication: SHELXL2016 (Sheldrick, 2015), PLATON (Spek, 2009) and publCIF (Westrip, 2010).

(S)-N-((S)-{(2S,4R,6R)-6-[(S)-2,3-Dihydroxypropyl]-4-hydroxy-5,5-dimethyltetrahydro-2H-pyran-2-yl}(hydroxy)methyl)-2-hydroxy-2-[(2R,5R,6R)-2-methoxy-5,6-dimethyl-4-methylenetetrahydro-2H-pyran-2-yl]acetamide methanol monosolvate top
Crystal data top
C22H39NO9·CH4OF(000) = 536
Mr = 493.58Dx = 1.263 Mg m−3
Monoclinic, P21Synchrotron radiation, λ = 0.9768 Å
a = 7.40 (5) ÅCell parameters from 7493 reflections
b = 12.87 (5) ŵ = 0.10 mm−1
c = 13.92 (5) ÅT = 100 K
β = 101.9 (5)°Needle, colorless
V = 1297 (11) Å30.20 × 0.02 × 0.01 mm
Z = 2
Data collection top
Single-axis goniometer
diffractometer		2610 independent reflections
Radiation source: synchrotron, CHESS F12594 reflections with I > 2σ(I)
Si 111 monochromatorRint = 0.054
Detector resolution: 5.8 pixels mm-1θmax = 31.0°, θmin = 2.1°
rotation scansh = −7→7
Absorption correction: empirical (using intensity measurements)
XDS (Kabsch, 2010), determined correction factors as a function of position on detector surface and frame number		k = −11→11
l = −13→13
7818 measured reflections
Refinement top
Refinement on F2Hydrogen site location: inferred from neighbouring sites
Least-squares matrix: fullH-atom parameters constrained
R[F2 > 2σ(F2)] = 0.044 w = 1/[σ2(Fo2) + (0.0824P)2 + 0.1123P]
where P = (Fo2 + 2Fc2)/3
wR(F2) = 0.110(Δ/σ)max = 0.003
S = 1.06Δρmax = 0.32 e Å−3
2610 reflectionsΔρmin = −0.20 e Å−3
309 parametersExtinction correction: (SHELXL2016; Sheldrick, 2015), Fc*=kFc[1+0.001xFc2λ3/sin(2θ)]-1/4
4 restraintsExtinction coefficient: 0.40 (2)
Special details top

Geometry. All esds (except the esd in the dihedral angle between two l.s. planes) are estimated using the full covariance matrix. The cell esds are taken into account individually in the estimation of esds in distances, angles and torsion angles; correlations between esds in cell parameters are only used when they are defined by crystal symmetry. An approximate (isotropic) treatment of cell esds is used for estimating esds involving l.s. planes.

Fractional atomic coordinates and isotropic or equivalent isotropic displacement parameters (Å2) top
xyzUiso*/Ueq
O10.9096 (3)0.2920 (2)0.80732 (18)0.0500 (8)
C20.9272 (6)0.2740 (4)0.9119 (3)0.0535 (11)
H21.0160170.3264200.9476910.064*
C211.0113 (7)0.1696 (4)0.9330 (3)0.0699 (14)
H21A1.0257100.1543011.0031090.105*
H21B0.9312230.1171440.8947200.105*
H21C1.1326120.1683100.9150280.105*
C30.7419 (6)0.2909 (4)0.9412 (3)0.0573 (12)
H30.7630700.2863551.0143810.069*
C310.5998 (8)0.2084 (4)0.8990 (4)0.0794 (15)
H31A0.6487690.1393790.9199530.119*
H31B0.4858120.2203470.9228380.119*
H31C0.5736180.2122800.8272170.119*
C40.6769 (5)0.3987 (3)0.9124 (3)0.0526 (12)
C410.6378 (7)0.4687 (4)0.9736 (3)0.0729 (14)
H41A0.6501730.4521281.0411370.087*
H41B0.5970330.5358480.9503790.087*
C50.6684 (5)0.4217 (4)0.8052 (3)0.0519 (11)
H5A0.6437710.4966150.7926310.062*
H5B0.5658250.3819750.7646280.062*
C60.8492 (5)0.3925 (3)0.7761 (3)0.0486 (11)
C611.1667 (6)0.4515 (5)0.8158 (4)0.0846 (16)
H61A1.2428620.5086560.8484000.127*
H61B1.2085820.3858640.8486780.127*
H61C1.1779430.4477880.7469300.127*
O60.9777 (4)0.4692 (2)0.8205 (2)0.0605 (9)
C70.8314 (5)0.3961 (4)0.6628 (3)0.0507 (11)
H70.9517630.3751210.6465230.061*
O70.7924 (4)0.4996 (3)0.6331 (2)0.0619 (9)
H7O0.8775260.5226510.6073870.093*
C80.6803 (5)0.3240 (4)0.6102 (3)0.0464 (11)
O80.6892 (4)0.2290 (3)0.6211 (2)0.0632 (9)
N90.5382 (4)0.3699 (3)0.5516 (2)0.0497 (9)
H90.5435160.4373290.5417380.060*
C100.3757 (5)0.3139 (4)0.5035 (3)0.0480 (10)
H100.4139870.2462400.4785000.058*
O100.2581 (4)0.2944 (3)0.5687 (2)0.0664 (9)
H10O0.1484290.2911160.5372690.100*
C110.2635 (5)0.3755 (3)0.4177 (3)0.0453 (10)
H110.1868630.4257830.4468860.054*
O110.3736 (3)0.43630 (19)0.36578 (17)0.0441 (7)
C120.1296 (6)0.3068 (4)0.3494 (3)0.0586 (12)
H12A0.0716870.2577170.3887940.070*
H12B0.0304830.3504590.3108110.070*
C130.2208 (6)0.2458 (3)0.2797 (3)0.0557 (12)
H130.3057410.1932980.3182820.067*
O130.0833 (5)0.1925 (3)0.2103 (3)0.0866 (12)
H13O0.0247010.1519190.2399590.130*
C140.3347 (6)0.3177 (4)0.2252 (3)0.0523 (11)
C1410.2083 (6)0.3926 (4)0.1573 (3)0.0640 (12)
H81A0.1416510.4361900.1962150.096*
H81B0.2826320.4368140.1231080.096*
H81C0.1196160.3529490.1091760.096*
C1420.4403 (8)0.2489 (4)0.1661 (4)0.0786 (15)
H82A0.5207140.2013110.2104930.118*
H82B0.3524260.2086920.1179260.118*
H82C0.5154420.2925570.1318580.118*
C150.4700 (5)0.3774 (3)0.3038 (3)0.0429 (9)
H150.5521060.3259720.3455880.052*
C160.5904 (6)0.4549 (4)0.2642 (3)0.0551 (11)
H16A0.6600850.4172110.2214830.066*
H16B0.5089350.5054380.2223980.066*
C170.7263 (5)0.5146 (3)0.3396 (3)0.0472 (10)
H170.7959820.4640830.3880300.057*
O170.6405 (4)0.5872 (3)0.3908 (3)0.0862 (12)
H17O0.6831990.6467020.3843830.129*
C180.8628 (6)0.5731 (4)0.2932 (3)0.0637 (13)
H18A0.9234330.5246900.2544030.076*
H18B0.7976920.6270210.2482680.076*
O180.9944 (5)0.6193 (3)0.3660 (3)0.0858 (12)
H18O1.0901570.6313270.3442770.129*
C501.2409 (8)0.6109 (6)0.5972 (4)0.0977 (19)
H50A1.2312280.6842170.6147180.147*
H50B1.3221780.6045240.5501590.147*
H50C1.2920650.5708150.6563950.147*
O501.0684 (5)0.5734 (3)0.5557 (3)0.0862 (11)
H50O1.0324800.6003680.5000970.103*
Atomic displacement parameters (Å2) top
U11U22U33U12U13U23
O10.0570 (16)0.055 (2)0.0380 (15)0.0062 (13)0.0094 (11)0.0020 (12)
C20.061 (2)0.059 (3)0.037 (2)−0.001 (2)0.0026 (17)0.0020 (18)
C210.086 (3)0.068 (4)0.054 (3)0.010 (2)0.012 (2)0.012 (2)
C30.073 (3)0.061 (3)0.039 (2)−0.008 (2)0.0138 (18)0.000 (2)
C310.086 (3)0.064 (4)0.093 (3)−0.019 (3)0.028 (3)−0.007 (3)
C40.054 (2)0.057 (3)0.049 (2)−0.006 (2)0.0142 (19)−0.007 (2)
C410.097 (3)0.071 (3)0.056 (3)0.003 (3)0.026 (2)−0.009 (2)
C50.051 (2)0.059 (3)0.044 (2)0.004 (2)0.0073 (18)−0.0005 (19)
C60.050 (2)0.055 (3)0.039 (2)0.000 (2)0.0058 (17)−0.001 (2)
C610.050 (3)0.103 (4)0.095 (4)−0.018 (3)0.001 (2)0.009 (3)
O60.0532 (17)0.063 (2)0.0605 (17)−0.0105 (14)0.0004 (13)−0.0011 (14)
C70.053 (2)0.054 (3)0.047 (2)0.0050 (19)0.0138 (18)0.007 (2)
O70.0651 (17)0.063 (2)0.0582 (17)−0.0057 (15)0.0128 (14)0.0127 (15)
C80.052 (2)0.050 (3)0.038 (2)0.010 (2)0.0116 (19)−0.0045 (19)
O80.068 (2)0.053 (3)0.0614 (19)0.0169 (15)−0.0022 (15)−0.0052 (15)
N90.056 (2)0.047 (2)0.0433 (18)0.0063 (16)0.0037 (17)0.0048 (16)
C100.051 (2)0.049 (2)0.045 (2)0.0039 (19)0.0124 (18)0.0049 (19)
O100.0692 (18)0.074 (2)0.0617 (18)0.0026 (16)0.0263 (15)0.0147 (15)
C110.046 (2)0.045 (2)0.046 (2)0.0022 (18)0.0104 (17)0.0072 (19)
O110.0536 (14)0.0383 (16)0.0401 (14)−0.0021 (12)0.0093 (12)0.0011 (11)
C120.048 (2)0.063 (3)0.061 (3)−0.004 (2)0.0028 (19)0.013 (2)
C130.063 (2)0.046 (3)0.051 (2)−0.012 (2)−0.006 (2)0.0020 (19)
O130.103 (3)0.063 (2)0.079 (2)−0.031 (2)−0.0153 (19)−0.0031 (17)
C140.066 (2)0.045 (2)0.043 (2)−0.002 (2)0.0035 (19)−0.0041 (19)
C1410.074 (3)0.060 (3)0.051 (2)−0.008 (2)−0.003 (2)0.003 (2)
C1420.100 (4)0.063 (4)0.074 (3)−0.008 (3)0.020 (3)−0.025 (3)
C150.051 (2)0.035 (2)0.042 (2)0.0022 (18)0.0093 (17)0.0024 (18)
C160.066 (2)0.055 (3)0.046 (2)−0.003 (2)0.0125 (19)0.003 (2)
C170.050 (2)0.043 (3)0.051 (2)−0.0006 (18)0.0158 (18)0.0007 (18)
O170.070 (2)0.069 (2)0.132 (3)−0.0264 (18)0.051 (2)−0.048 (2)
C180.061 (3)0.063 (3)0.072 (3)−0.008 (2)0.026 (2)0.004 (2)
O180.0644 (19)0.103 (3)0.090 (3)−0.033 (2)0.0148 (18)0.007 (2)
C500.080 (4)0.127 (6)0.081 (4)−0.021 (4)0.003 (3)0.000 (3)
O500.084 (2)0.098 (3)0.079 (2)−0.023 (2)0.0224 (19)0.006 (2)
Geometric parameters (Å, º) top
O1—C61.407 (7)C11—O111.429 (7)
O1—C21.454 (7)C11—C121.511 (9)
C2—C211.485 (9)C11—H111.0000
C2—C31.525 (11)O11—C151.443 (7)
C2—H21.0000C12—C131.510 (9)
C21—H21A0.9800C12—H12A0.9900
C21—H21B0.9800C12—H12B0.9900
C21—H21C0.9800C13—O131.427 (9)
C3—C41.496 (9)C13—C141.552 (8)
C3—C311.525 (9)C13—H131.0000
C3—H31.0000O13—H13O0.8400
C31—H31A0.9800C14—C1411.527 (9)
C31—H31B0.9800C14—C1421.528 (9)
C31—H31C0.9800C14—C151.529 (9)
C4—C411.312 (7)C141—H81A0.9800
C4—C51.510 (8)C141—H81B0.9800
C41—H41A0.9500C141—H81C0.9800
C41—H41B0.9500C142—H82A0.9800
C5—C61.523 (11)C142—H82B0.9800
C5—H5A0.9900C142—H82C0.9800
C5—H5B0.9900C15—C161.515 (8)
C6—O61.421 (8)C15—H151.0000
C6—C71.557 (8)C16—C171.507 (9)
C61—O61.431 (11)C16—H16A0.9900
C61—H61A0.9800C16—H16B0.9900
C61—H61B0.9800C17—O171.403 (7)
C61—H61C0.9800C17—C181.508 (9)
C7—O71.406 (8)C17—H171.0000
C7—C81.520 (9)O17—H17O0.8400
C7—H71.0000C18—O181.387 (10)
O7—H7O0.8400C18—H18A0.9900
C8—O81.231 (7)C18—H18B0.9900
C8—N91.330 (9)O18—H18O0.8400
N9—C101.444 (9)C50—O501.376 (11)
N9—H90.8800C50—H50A0.9800
C10—O101.403 (9)C50—H50B0.9800
C10—C111.528 (9)C50—H50C0.9800
C10—H101.0000O50—H50O0.8400
O10—H10O0.8400
C6—O1—C2114.4 (3)O11—C11—C10113.9 (5)
O1—C2—C21106.8 (4)C12—C11—C10111.7 (5)
O1—C2—C3110.2 (5)O11—C11—H11106.3
C21—C2—C3116.1 (4)C12—C11—H11106.3
O1—C2—H2107.8C10—C11—H11106.3
C21—C2—H2107.8C11—O11—C15114.7 (4)
C3—C2—H2107.8C13—C12—C11112.7 (5)
C2—C21—H21A109.5C13—C12—H12A109.0
C2—C21—H21B109.5C11—C12—H12A109.0
H21A—C21—H21B109.5C13—C12—H12B109.0
C2—C21—H21C109.5C11—C12—H12B109.0
H21A—C21—H21C109.5H12A—C12—H12B107.8
H21B—C21—H21C109.5O13—C13—C12109.5 (5)
C4—C3—C2108.5 (4)O13—C13—C14109.8 (4)
C4—C3—C31112.6 (5)C12—C13—C14111.3 (5)
C2—C3—C31112.6 (5)O13—C13—H13108.7
C4—C3—H3107.6C12—C13—H13108.7
C2—C3—H3107.6C14—C13—H13108.7
C31—C3—H3107.6C13—O13—H13O109.5
C3—C31—H31A109.5C141—C14—C142110.4 (5)
C3—C31—H31B109.5C141—C14—C15110.7 (5)
H31A—C31—H31B109.5C142—C14—C15110.1 (5)
C3—C31—H31C109.5C141—C14—C13110.7 (5)
H31A—C31—H31C109.5C142—C14—C13107.9 (5)
H31B—C31—H31C109.5C15—C14—C13106.9 (4)
C41—C4—C3124.3 (5)C14—C141—H81A109.5
C41—C4—C5122.5 (5)C14—C141—H81B109.5
C3—C4—C5113.1 (4)H81A—C141—H81B109.5
C4—C41—H41A120.0C14—C141—H81C109.5
C4—C41—H41B120.0H81A—C141—H81C109.5
H41A—C41—H41B120.0H81B—C141—H81C109.5
C4—C5—C6110.8 (5)C14—C142—H82A109.5
C4—C5—H5A109.5C14—C142—H82B109.5
C6—C5—H5A109.5H82A—C142—H82B109.5
C4—C5—H5B109.5C14—C142—H82C109.5
C6—C5—H5B109.5H82A—C142—H82C109.5
H5A—C5—H5B108.1H82B—C142—H82C109.5
O1—C6—O6111.2 (5)O11—C15—C16105.8 (5)
O1—C6—C5112.7 (4)O11—C15—C14111.1 (5)
O6—C6—C5105.0 (5)C16—C15—C14114.7 (4)
O1—C6—C7107.2 (4)O11—C15—H15108.4
O6—C6—C7109.3 (5)C16—C15—H15108.4
C5—C6—C7111.5 (5)C14—C15—H15108.4
O6—C61—H61A109.5C17—C16—C15116.1 (4)
O6—C61—H61B109.5C17—C16—H16A108.3
H61A—C61—H61B109.5C15—C16—H16A108.3
O6—C61—H61C109.5C17—C16—H16B108.3
H61A—C61—H61C109.5C15—C16—H16B108.3
H61B—C61—H61C109.5H16A—C16—H16B107.4
C6—O6—C61116.2 (5)O17—C17—C16112.7 (5)
O7—C7—C8110.5 (5)O17—C17—C18107.4 (5)
O7—C7—C6107.2 (4)C16—C17—C18111.4 (5)
C8—C7—C6111.7 (5)O17—C17—H17108.4
O7—C7—H7109.1C16—C17—H17108.4
C8—C7—H7109.1C18—C17—H17108.4
C6—C7—H7109.1C17—O17—H17O109.5
C7—O7—H7O109.5O18—C18—C17109.3 (5)
O8—C8—N9122.2 (4)O18—C18—H18A109.8
O8—C8—C7122.1 (5)C17—C18—H18A109.8
N9—C8—C7115.7 (6)O18—C18—H18B109.8
C8—N9—C10122.7 (5)C17—C18—H18B109.8
C8—N9—H9118.6H18A—C18—H18B108.3
C10—N9—H9118.6C18—O18—H18O109.5
O10—C10—N9110.9 (5)O50—C50—H50A109.5
O10—C10—C11106.8 (5)O50—C50—H50B109.5
N9—C10—C11111.8 (5)H50A—C50—H50B109.5
O10—C10—H10109.1O50—C50—H50C109.5
N9—C10—H10109.1H50A—C50—H50C109.5
C11—C10—H10109.1H50B—C50—H50C109.5
C10—O10—H10O109.5C50—O50—H50O109.5
O11—C11—C12111.7 (4)
C6—O1—C2—C21−173.8 (3)C8—N9—C10—O1078.8 (5)
C6—O1—C2—C359.3 (4)C8—N9—C10—C11−162.2 (3)
O1—C2—C3—C4−57.1 (4)O10—C10—C11—O11156.4 (4)
C21—C2—C3—C4−178.6 (4)N9—C10—C11—O1135.0 (5)
O1—C2—C3—C3168.3 (6)O10—C10—C11—C12−75.9 (5)
C21—C2—C3—C31−53.3 (6)N9—C10—C11—C12162.7 (3)
C2—C3—C4—C41−122.7 (6)C12—C11—O11—C15−53.8 (5)
C31—C3—C4—C41111.9 (6)C10—C11—O11—C1573.9 (5)
C2—C3—C4—C554.6 (6)O11—C11—C12—C1349.2 (5)
C31—C3—C4—C5−70.7 (6)C10—C11—C12—C13−79.7 (5)
C41—C4—C5—C6127.0 (5)C11—C12—C13—O13−173.2 (3)
C3—C4—C5—C6−50.4 (5)C11—C12—C13—C14−51.6 (5)
C2—O1—C6—O662.7 (6)O13—C13—C14—C14155.7 (6)
C2—O1—C6—C5−54.9 (5)C12—C13—C14—C141−65.8 (5)
C2—O1—C6—C7−177.9 (3)O13—C13—C14—C142−65.2 (5)
C4—C5—C6—O148.9 (5)C12—C13—C14—C142173.3 (4)
C4—C5—C6—O6−72.3 (5)O13—C13—C14—C15176.3 (3)
C4—C5—C6—C7169.5 (4)C12—C13—C14—C1554.9 (5)
O1—C6—O6—C6149.5 (5)C11—O11—C15—C16−174.7 (3)
C5—C6—O6—C61171.7 (4)C11—O11—C15—C1460.2 (4)
C7—C6—O6—C61−68.6 (6)C141—C14—C15—O1162.4 (4)
O1—C6—C7—O7−173.2 (3)C142—C14—C15—O11−175.2 (3)
O6—C6—C7—O7−52.5 (5)C13—C14—C15—O11−58.3 (5)
C5—C6—C7—O763.1 (4)C141—C14—C15—C16−57.5 (5)
O1—C6—C7—C865.6 (5)C142—C14—C15—C1664.9 (6)
O6—C6—C7—C8−173.7 (3)C13—C14—C15—C16−178.2 (3)
C5—C6—C7—C8−58.1 (6)O11—C15—C16—C1757.6 (5)
O7—C7—C8—O8176.5 (3)C14—C15—C16—C17−179.6 (4)
C6—C7—C8—O8−64.2 (5)C15—C16—C17—O17−70.4 (6)
O7—C7—C8—N9−3.3 (4)C15—C16—C17—C18168.8 (4)
C6—C7—C8—N9115.9 (5)O17—C17—C18—O1861.3 (5)
O8—C8—N9—C106.0 (5)C16—C17—C18—O18−174.8 (4)
C7—C8—N9—C10−174.1 (3)
Hydrogen-bond geometry (Å, º) top
D—H···AD—HH···AD···AD—H···A
N9—H9···O70.882.162.594 (12)109
O7—H7O···O500.841.832.672 (15)178
O50—H50O···O180.841.852.651 (11)160
O13—H13O···O6i0.842.502.927 (12)113
O17—H17O···O10ii0.842.032.796 (11)152
O18—H18O···O8iii0.842.042.708 (14)136
Symmetry codes: (i) −x+1, y−1/2, −z+1; (ii) −x+1, y+1/2, −z+1; (iii) −x+2, y+1/2, −z+1.
 

Acknowledgements

The authors thank Kathie Moulton (USDA–Ft Pierce) for providing insects, and Aaron Finke (MacCHESS) and Ivan Keresztes (Cornell Chemistry and Biology) for helpful discussions.

Funding information

Funding for this research was provided by: National Institutes of Health (award No. GM-103485 to the MacCHESS Facility); National Science Foundation (award No. DMR-133208 to the CHESS facility; award No. CHE-1531632 to Cornell University NMR Facility); California Citrus Research Board (grant No. 5300-155 to Michelle L. Heck); National Institute of Food and Agriculture (United States) (grant No. 60-8062-6-002 to Michelle L. Heck).

References

First citationBové, J. M. (2006). J. Plant Pathol. 7–37.  Google Scholar
 First citationBurnett, M. N. & Johnson, C. K. (1996). ORTEPIII. Report ORNL-6895. Oak Ridge National Laboratory, Tennessee, USA.  Google Scholar
 First citationCardani, C., Ghiringhelli, D., Mondelli, R. & Quilico, A. (1965). Tetrahedron Lett. 6, 2537–2545.  CrossRef Google Scholar
 First citationCardani, C., Ghiringhelli, D., Quilico, A. & Selva, A. (1967). Tetrahedron Lett. 8, 4023–4025.  CrossRef Google Scholar
 First citationCorradi, A. B., Mangia, A., Nadelli, M. & Pelizzi, G. (1971). Gazz. Chim. Ital. 101, 591.  Google Scholar
 First citationEmsley, P., Lohkamp, B., Scott, W. G. & Cowtan, K. (2010). Acta Cryst. D66, 486–501.  Web of Science CrossRef CAS IUCr Journals Google Scholar
 First citationFurusaki, A., Watanabé, T., Matsumoto, T. & Yanagiya, M. (1968). Tetrahedron Lett. 9, 6301–6304.  CSD CrossRef Google Scholar
 First citationGroom, C. R., Bruno, I. J., Lightfoot, M. P. & Ward, S. C. (2016). Acta Cryst. B72, 171–179.  Web of Science CSD CrossRef IUCr Journals Google Scholar
 First citationKabsch, W. (2010). Acta Cryst. D66, 125–132.  Web of Science CrossRef CAS IUCr Journals Google Scholar
 First citationMatsumoto, T., Yanagiya, M., Maeno, S. & Yasuda, S. (1968). Tetrahedron Lett. 9, 6297–6300.  CrossRef Google Scholar
 First citationMosey, R. A. & Floreancig, P. E. (2012). Nat. Prod. Rep. 29, 980–995.  Web of Science CrossRef CAS Google Scholar
 First citationNakabachi, A., Ueoka, R., Oshima, K., Teta, R., Mangoni, A., Gurgui, M., Oldham, N. J., van Echten-Deckert, G., Okamura, K., Yamamoto, K., Inoue, H., Ohkuma, M., Hongoh, Y., Miyagishima, S. Y., Hattori, M., Piel, J. & Fukatsu, T. (2013). Curr. Biol. 23, 1478–1484.  Web of Science CrossRef CAS Google Scholar
 First citationOtwinowski, Z. & Minor, W. (1997). Methods in Enzymology, Vol. 276, Macromolecular Crystallography, Part A, edited by C. W. Carter Jr & R. M. Sweet, pp. 307–326. New York: Academic Press.  Google Scholar
 First citationPiel, J. (2002). Proc. Natl Acad. Sci. USA, 99, 14002–14007.  Web of Science CrossRef CAS Google Scholar
 First citationSheldrick, G. M. (2015). Acta Cryst. C71, 3–8.  Web of Science CrossRef IUCr Journals Google Scholar
 First citationSpek, A. L. (2009). Acta Cryst. D65, 148–155.  Web of Science CrossRef CAS IUCr Journals Google Scholar
 First citationSzebenyi, D. M. E., Arvai, A., Ealick, S., LaIuppa, J. M. & Nielsen, C. (1997). J. Synchrotron Rad. 4, 128–135.  CrossRef CAS Web of Science IUCr Journals Google Scholar
 First citationWang, N., Stelinski, L. L., Pelz-Stelinski, K. S., Graham, J. H. & Zhang, Y. (2017). Phytopathology, 107, 380–387.  Web of Science CrossRef Google Scholar
 First citationWeeks, C. M. & Miller, R. (1999a). J. Appl. Cryst. 32, 120-124.  Web of Science CrossRef CAS IUCr Journals Google Scholar
 First citationWeeks, C. M. & Miller, R. (1999b). Acta Cryst. D55, 492–500.  Web of Science CrossRef CAS IUCr Journals Google Scholar
 First citationWestrip, S. P. (2010). J. Appl. Cryst. 43, 920–925.  Web of Science CrossRef CAS IUCr Journals Google Scholar

This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.

Journal logoCRYSTALLOGRAPHIC
COMMUNICATIONS
ISSN: 2056-9890
Volume 74| Part 4| April 2018| Pages 445-449
https://doi.org/10.1107/S2056989018002992
Follow Acta Cryst. E
Sign up for e-alerts
E-alerts
Follow Acta Cryst. on Twitter
Twitter
Follow us on facebook
Facebook
Sign up for RSS feeds
RSS