Synthesis, crystal structure and Hirshfeld surface analysis of 4-[3-(4-hy­droxy­phen­yl)-4,5-di­hydro-1H-pyrazol-5-yl]-2-meth­oxy­phenol monohydrate

Duong Khanh, L.; Hanh Trinh Thi, M.; Quynh Bui Thi, T.; Vu Quoc, T.; Nguyen Thien, V.; Van Meervelt, L.

doi:10.1107/S2056989019013379

research communications

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ISSN: 2056-9890

Volume 75| Part 11| November 2019| Pages 1590-1594

https://doi.org/10.1107/S2056989019013379

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Synthesis, crystal structure and Hirshfeld surface analysis of 4-[3-(4-hydroxyphenyl)-4,5-dihydro-1H-pyrazol-5-yl]-2-methoxyphenol monohydrate

Linh Duong Khanh,^a ^* My Hanh Trinh Thi,^a Thuy Quynh Bui Thi,^a Trung Vu Quoc,^a Vuong Nguyen Thien ^b,^c and Luc Van Meervelt ^d ^*

^aFaculty of Chemistry, Hanoi National University of Education, 136 Xuan Thuy, Cau Giay, Hanoi, Vietnam, ^bInstitute for Tropical Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam, ^cGraduate University of Science and Technology, VAST, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam, and ^dDepartment of Chemistry, KU Leuven, Biomolecular Architecture, Celestijnenlaan 200F, Leuven (Heverlee), B-3001, Belgium
^*Correspondence e-mail: linhddk@yahoo.com, luc.vanmeervelt@kuleuven.be

Edited by J. Simpson, University of Otago, New Zealand (Received 24 September 2019; accepted 30 September 2019; online 3 October 2019)

In the title pyrazoline derivative, C₁₆H₁₆N₂O₃·H₂O, the pyrazoline ring has an envelope conformation with the substituted sp² C atom on the flap. The pyrazoline ring makes angles of 86.73 (12) and 13.44 (12)° with the trisubstituted and disubstituted benzene rings, respectively. In the crystal structure, the molecules are connected into chains running in the b-axis direction by O—H⋯N hydrogen bonding. Parallel chains interact through N—H⋯O hydrogen bonds and π–π stacking of the trisubstituted phenyl rings. The major contribution to the surface contacts are H⋯H contacts (44.3%) as concluded from a Hirshfeld surface analysis.

Keywords: crystal structure; pyrazolines; hydrogen bonding; Hirshfeld analysis.

CCDC references: 1956698; 1956698

1. Chemical context

Chalcones are one of the most important classes of flavonoids. Natural and synthetic chalcone derivatives have shown a variety of promising biological activities such as anti-inflammatory, anti-gout, anti-histaminic, anti-oxidant, anti-obesity, anti-protozoal, hypnotic and anti-spasmodic activities (Gomes et al., 2017 ). Heterocyclic compounds including pyrazolines can be synthesized from chalcone derivatives. Many compounds containing pyrazolines show different biological activities and are known to act as anticancer (Johnson et al., 2007 ; Gomha et al., 2017 ), antimicrobial (Patel et al., 2016 ), antitubercular (Taj et al., 2011 ), anti-inflammatory (Malhotra et al., 2010 ), anticonvulsant (Siddiqui et al., 2009 ), anti-amoebic (Bhat et al., 2009 ), antioxidant (Srinivasan et al., 2007 ), antiviral (Gomha et al., 2016 ), antibacterial (Kumar et al., 2008 ) and antinociceptive (Kaplancikli et al., 2009 ) agents.

Pyrazoline derivatives have been synthesized by condensation of chalcones with hydrazine derivatives using conventional synthesis (Shahare et al., 2009 ; Sridhar et al., 2012 ) and microwave-assisted synthesis (Kumar et al., 2008; Patel et al., 2016).

In this article, we report the synthesis of a chalcone derivative by condensation of vanillin with p-hydroxyacetophenone and subsequent cyclization of this chalcone by reaction with hydrazine hydrate. Furthermore the molecular and crystal structure of the title compound, 2, are presented together with a Hirshfeld surface analysis and non-covalent interaction plots.

2. Structural commentary

The title compound crystallizes in the orthorhombic space group Pbca with one molecule and a water molecule in the asymmetric unit (Fig. 1). The pyrazoline ring (N1/N2/C3–C5; r.m.s. deviation = 0.078 Å) is slightly twisted on N2—C3 [puckering parameters: Q(2) = 0.175 (2) Å, Φ(2) = 60.7 (7)°]. There is a clear difference in both C—N bond distances in the pyrazoline ring: N1=C5 shows double-bond character [1.287 (3) Å] while N2—C3 [1.496 (3) Å] is a single bond. The dihedral angle between the two benzene rings is 80.66 (11)°. The planes of the C6–C11 benzene ring (r.m.s. deviation = 0.004 Å) and the pyrazoline ring make an angle of 86.73 (12)°. For the C15–C20 benzene ring (r.m.s. deviation = 0.006 Å), the dihedral angle with the pyrazoline ring is only 13.44 (12)°. Both the hydroxy and methoxy substituents of the C6–C11 phenyl group are within the phenyl plane with deviations of 0.011 (1) (O12), 0.166 (2) (C13) and −0.057 (2) Å (O14).

Figure 1
A view of the molecular structure of 2, with atom labels and displacement ellipsoids drawn at the 50% probability level. H atoms are shown as small circles of arbitrary radii and the O—H⋯N interaction as a dotted blue line.

3. Supramolecular features and Hirshfeld surface analysis

In the crystal of 2, the O22 water molecule bridges three molecules by O—H⋯N and O—H⋯O hydrogen-bonding interactions with the N1 atom and the O21-hydroxy group (Fig. 2, Table 1). The pyrazoline N2 atom acts as a hydrogen-bond acceptor to the second O14-hydroxy group, resulting in chain formation along the b-axis direction (Fig. 3, Table 1). Parallel chains linked by inversion interact in two different ways. First, the N2 hydrogen atom acts also as hydrogen-bond donor to the O12-methoxy group. In addition, both chains interact by π–π stacking [Cg1⋯Cg1(−x + 1, −y + 1, −z + 1) = 3.6627 (11) Å; slippage 1.442 Å; Cg1 is the centroid of the C6–C11 ring]. In addition, a C—H⋯O interaction is observed in the crystal packing (Table 1). No voids are observed in the crystal packing of 2.

Table 1
Hydrogen-bond geometry (Å, °)

D—H⋯A	D—H	H⋯A	D⋯A	D—H⋯A
N2—H2⋯O12ⁱ	0.87 (2)	2.36 (2)	3.209 (2)	167 (2)
O14—H14⋯N2ⁱⁱ	0.91 (3)	1.89 (3)	2.760 (2)	158 (3)
O21—H21⋯O22ⁱⁱⁱ	0.98 (4)	1.66 (4)	2.633 (3)	169 (4)
O22—H22A⋯O21^iv	0.91 (4)	1.99 (4)	2.891 (3)	171 (3)
O22—H22B⋯N1	0.98 (4)	1.84 (4)	2.794 (3)	166 (3)
C7—H7⋯O12^v	0.93	2.56	3.465 (2)	165
Symmetry codes: (i) -x+1, -y+1, -z+1; (ii) x, y-1, z; (iii) $[x+{\script{1\over 2}}, y, -z+{\script{1\over 2}}]$ ; (iv) $[-x+1, y-{\script{1\over 2}}, -z+{\script{1\over 2}}]$ ; (v) $[-x+{\script{3\over 2}}, y+{\script{1\over 2}}, z]$ .

Figure 2
Partial crystal packing of 2, showing the interactions of water molecule O22. O—H⋯N and O—H⋯O interactions are shown as blue and red dashed lines, respectively (see Table 1

for symmetry codes).

Figure 3
Partial crystal packing of 2, showing the chain formation along the b axis by O—H⋯N interactions (blue dashed lines). Parallel chains are linked by N—H⋯O (red dashed lines) and π–π interactions (grey dashed lines; Cg1 is the centroid of the C6–C11 ring; see Table 1

for symmetry codes).

The Hirshfeld surface (calculated using CrystalExplorer; Turner et al., 2017 ) mapped over d_norm in Fig. 4 also gives the usual indications of these intermolecular interactions through the appearance of bright-red spots near participating atoms (Spackman & Jayatilaka, 2009 ). In addition to the interactions already discussed, faint-red spots near atoms C8, C11, H3 and H4A illustrate short C⋯H contacts (H4A⋯C11 = 2.83 Å, H3⋯C8 = 2.80 Å). The associated two-dimensional fingerprint plots (McKinnon et al., 2007 ) were used to further explore the intermolecular contacts (Fig. 5) and indicate that the major contribution is from H⋯H contacts, corresponding to 44.3% of the fingerprint plot (Fig. 5b) followed by reciprocal C⋯H/H⋯C contacts (25.1%, Fig. 5c). Significant contributions come from reciprocal O⋯H/H⋯O (20.7%) and N⋯H/H⋯N (7.0%) contacts, which appear as two symmetrical spikes at d_e + d_i = 1.65 and 1.80 Å, respectively (Fig. 5d,e). A further small contribution is from C⋯C contacts (2.3%, Fig. 5f).

Figure 4
Two views of the Hirshfeld surface mapped over d_norm for 2 in the range −0.7348 to +1.5269 arbitrary units.

Figure 5
Full two-dimensional fingerprint plots for 2, showing (a) all interactions, and delineated into (b) H⋯H, (c) C⋯H/H⋯C, (d) O⋯H/H⋯O, (e) N⋯H/H⋯N and (f) C⋯C interactions. The d_i and d_e values are the closest internal and external distances (in Å) from a given point on the Hirshfeld surface.

Based on the Hirshfeld surface analysis, enrichment ratios (ER, Table 2) were calculated by comparing the contacts in the crystal with those computed as if all types of contact have the same probability of forming (Jelsch et al., 2014 ). A ratio greater than unity for a pair of elements indicates a high likelihood of forming contacts in the crystal. This is the case for N⋯H and O⋯H contacts, which is consistent with the high propensity for the formation of O—H⋯N and O/N/C—H⋯O hydrogen bonds. C⋯H contacts are enriched because of the presence of aromatic rings, H⋯H contacts are found to have the usual enrichment ratios slightly lower than unity.

Table 2
Enrichment ratios for 2

Parameter	Ratio
H⋯H	0.89
C⋯H	1.18
O⋯H	1.39
N⋯H	1.41
C⋯C	1.02

4. Database survey

A search of the Cambridge Structural Database (CSD, Version 5.40, update of May 2019; Groom et al., 2016 ) for 2-pyrazoline derivatives gave 134 hits, of which 37 are 3,5-disubstituted (only organic molecules were considered). Where both substituents on the pyrazoline ring are aromatic rings, three 2-pyrazoline derivatives were found with substituted benzene rings at position 3 and a 2-naphthyl ring system at position 5. In addition, two structures have substituted benzene rings as both substituents and are very similar to 2. The first one, 2-methoxy-4-[3-(3-nitrophenyl)-4,5-dihydro-1H-pyrazol-5-yl]phenol (refcode UJUDOU; Inturi et al., 2016 ), crystallizes in P2₁/c with one molecule in the asymmetric unit. The pyrazoline ring has an envelope conformation with the substituted sp² C atom on the flap. The dihedral angle between the phenyl rings is 49.37 (8)°, that between the pyrazoline ring and the nitrophenyl ring is 9.7 (1)° and that between the pyrazoline ring and the methoxyphenol ring is 56. 78 (9)°. The second structure, 3-(2′-hydroxy-5′-methoxy-phenyl)-5-(3-methoxy-4-hydroxyphenyl)-4,5-dihydro-1H-pyrazole (RESJUV; Gupta et al., 2006 ), crystallizes in Pbca with one molecule in the asymmetric unit. The conformation of the pyrazoline ring is the same as that in UJUDOU. The phenyl rings make an angle of 56.0 (1)°, while the dihedral angles between the pyrazoline ring and the phenyl rings at atom C3 and C5 are 12.1 (1) and 68.2 (1)°, respectively.

5. Synthesis and crystallization

The reaction scheme for the synthesis of 2 starting from vanillin is given in Fig. 6. (E)-3-(4-Hydroxy-3-methoxyphenyl)-1-(4-hydroxyphenyl)prop-2-en-1-one, 1, was synthesized as described in a previous study (Duong Khanh et al., 2018 ).

Figure 6
Reaction scheme for the synthesis of compound 2.

Synthesis of 4-(3-(4-hydroxyphenyl)-4,5-dihydro-1H-pyrazol-5-yl)-2-methoxyphenol (2):

A mixture of chalcone 1 (0.01 mol), 2.5 mL of hydrazine hydrate and 25 mL of ethanol was refluxed at 353 K for 2 h. After pouring the reaction mixture into 200 mL of ice–water, the crude solid product was isolated by vacuum filtration, washed several times with cold water and recrystallized from ethanol:water (1:2) to give yellow crystals (2.24 g, yield 79%), m.p. 465 K. ¹H NMR [Bruker XL-500, 500 MHz, d₆-DMSO, δ (ppm), J (Hz), see Fig. 6 for numbering scheme]: 6.71 (d, 1H, J = 8.0, H2); 3.75 (s, 3H, H2a); 6.74 (d, 1H, J = 1.5, H3); 6.95 (d, 1H, J = 1.5, H5), 4.67 (t, 1H, H7); 3.30 (dd, 1H, J = 11.0; 16.0, H8a); 2.76 (dd, 1H, J = 11.0, 16.0, H8b), 7.45 (d, 2H, J = 8.5, H11 and H15); 6.76 (d, 2H, J = 8.5, H12 and H14).

6. Refinement

Crystal data, data collection and structure refinement details are summarized in Table 3. The O- and N-bound H atoms H2, H14, H21, H22A and H22B were found in difference electron density maps and refined freely. The other H atoms were placed in idealized positions and included as riding contributions with U_iso(H) values of 1.2U_eq or 1.5U_eq of the parent atoms, with C—H distances of 0.93 (aromatic), 0.98 (CH), 0.97 (CH₂) and 0.96 Å (CH₃). In the final cycles of refinement, eight outliers were omitted.

Table 3
Experimental details

Crystal data
Chemical formula	C₁₆H₁₆N₂O₃·H₂O
M_r	302.32
Crystal system, space group	Orthorhombic, Pbca
Temperature (K)	293
a, b, c (Å)	12.1452 (5), 8.1784 (3), 31.2738 (12)
V (Å³)	3106.4 (2)
Z	8
Radiation type	Mo Kα
μ (mm⁻¹)	0.09
Crystal size (mm)	0.5 × 0.2 × 0.05

Data collection
Diffractometer	Rigaku Oxford Diffraction SuperNova, Single source at offset/far, Eos
Absorption correction	Multi-scan (CrysAlis PRO; Rigaku OD, 2018 $[Rigaku OD (2018). CrysAlis PRO. Rigaku Oxford Diffraction, Yarnton, UK.]$ )
T_min, T_max	0.697, 1.000
No. of measured, independent and observed [I > 2σ(I)] reflections	16817, 3172, 2241
R_int	0.039
(sin θ/λ)_max (Å⁻¹)	0.625

Refinement
R[F² > 2σ(F²)], wR(F²), S	0.050, 0.115, 1.03
No. of reflections	3172
No. of parameters	220
H-atom treatment	H atoms treated by a mixture of independent and constrained refinement
Δρ_max, Δρ_min (e Å⁻³)	0.18, −0.19
Computer programs: CrysAlis PRO (Rigaku OD, 2018 $[Rigaku OD (2018). CrysAlis PRO. Rigaku Oxford Diffraction, Yarnton, UK.]$ ), SHELXT2014 (Sheldrick, 2015a ), SHELXL2016 (Sheldrick, 2015b ) and OLEX2 (Dolomanov et al., 2009 ).

Supporting information

CCDC references: 1956698; 1956698

Crystal structure: contains datablock I. DOI: https://doi.org/10.1107/S2056989019013379/sj5579sup1.cif

Structure factors: contains datablock I. DOI: https://doi.org/10.1107/S2056989019013379/sj5579Isup2.hkl

Supporting information file. DOI: https://doi.org/10.1107/S2056989019013379/sj5579Isup3.cml

checkCIF report

Computing details top

Data collection: CrysAlis PRO (Rigaku OD, 2018); cell refinement: CrysAlis PRO (Rigaku OD, 2018); data reduction: CrysAlis PRO (Rigaku OD, 2018); program(s) used to solve structure: SHELXT2014 (Sheldrick, 2015a); program(s) used to refine structure: SHELXL2016 (Sheldrick, 2015b); molecular graphics: OLEX2 (Dolomanov et al., 2009); software used to prepare material for publication: OLEX2 (Dolomanov et al., 2009).

4-[3-(4-Hydroxyphenyl)-4,5-dihydro-1H-pyrazol-5-yl]-2-methoxyphenol monohydrate top

Crystal data top

C₁₆H₁₆N₂O₃·H₂O	D_x = 1.293 Mg m⁻³
M_r = 302.32	Mo Kα radiation, λ = 0.71073 Å
Orthorhombic, Pbca	Cell parameters from 4406 reflections
a = 12.1452 (5) Å	θ = 3.1–24.8°
b = 8.1784 (3) Å	µ = 0.09 mm⁻¹
c = 31.2738 (12) Å	T = 293 K
V = 3106.4 (2) Å³	Plate, yellow
Z = 8	0.5 × 0.2 × 0.05 mm
F(000) = 1280

Data collection top

Rigaku Oxford Diffraction SuperNova, Single source at offset/far, Eos diffractometer	3172 independent reflections
Radiation source: micro-focus sealed X-ray tube, SuperNova (Mo) X-ray Source	2241 reflections with I > 2σ(I)
Mirror monochromator	R_int = 0.039
Detector resolution: 15.9631 pixels mm^-1	θ_max = 26.4°, θ_min = 2.6°
ω scans	h = −15→15
Absorption correction: multi-scan (CrysAlis PRO; Rigaku OD, 2018)	k = −10→9
T_min = 0.697, T_max = 1.000	l = −38→39
16817 measured reflections

Refinement top

Refinement on F²	0 restraints
Least-squares matrix: full	Hydrogen site location: mixed
R[F² > 2σ(F²)] = 0.050	H atoms treated by a mixture of independent and constrained refinement
wR(F²) = 0.115	w = 1/[σ²(F_o²) + (0.0321P)² + 1.8202P] where P = (F_o² + 2F_c²)/3
S = 1.03	(Δ/σ)_max < 0.001
3172 reflections	Δρ_max = 0.18 e Å⁻³
220 parameters	Δρ_min = −0.19 e Å⁻³

Special details top

Geometry. All esds (except the esd in the dihedral angle between two l.s. planes) are estimated using the full covariance matrix. The cell esds are taken into account individually in the estimation of esds in distances, angles and torsion angles; correlations between esds in cell parameters are only used when they are defined by crystal symmetry. An approximate (isotropic) treatment of cell esds is used for estimating esds involving l.s. planes.

Fractional atomic coordinates and isotropic or equivalent isotropic displacement parameters (Å²) top

	x	y	z	U_iso*/U_eq
O12	0.67375 (11)	0.32986 (16)	0.53007 (4)	0.0374 (4)
O14	0.56063 (13)	0.13497 (17)	0.48138 (5)	0.0433 (4)
N2	0.51402 (15)	0.8855 (2)	0.42476 (5)	0.0347 (4)
N1	0.49999 (14)	0.9151 (2)	0.38046 (5)	0.0384 (4)
O22	0.29208 (18)	0.8202 (3)	0.35137 (6)	0.0677 (5)
O21	0.64259 (19)	1.0090 (3)	0.18438 (6)	0.0803 (7)
C8	0.63357 (15)	0.3973 (2)	0.49290 (6)	0.0287 (4)
C9	0.57465 (16)	0.2905 (2)	0.46661 (6)	0.0306 (4)
C7	0.64750 (15)	0.5586 (2)	0.48084 (6)	0.0298 (4)
H7	0.686502	0.629245	0.498546	0.036*
C6	0.60392 (16)	0.6168 (2)	0.44254 (6)	0.0312 (4)
C11	0.54679 (16)	0.5094 (2)	0.41631 (6)	0.0346 (5)
H11	0.517910	0.546425	0.390526	0.042*
C3	0.61976 (16)	0.7953 (2)	0.43154 (7)	0.0348 (5)
H3	0.660239	0.848056	0.454879	0.042*
C10	0.53234 (17)	0.3472 (2)	0.42823 (6)	0.0354 (5)
H10	0.494061	0.276326	0.410349	0.042*
C5	0.59163 (17)	0.8914 (2)	0.36073 (7)	0.0376 (5)
C15	0.60588 (19)	0.9252 (3)	0.31515 (7)	0.0431 (5)
C4	0.68056 (18)	0.8284 (3)	0.38953 (7)	0.0450 (6)
H4A	0.738080	0.909351	0.393418	0.054*
H4B	0.712858	0.728975	0.378196	0.054*
C13	0.7444 (2)	0.4300 (3)	0.55573 (7)	0.0482 (6)
H13A	0.803642	0.471013	0.538454	0.072*
H13B	0.773932	0.366112	0.578791	0.072*
H13C	0.703076	0.520021	0.567146	0.072*
C16	0.6949 (2)	0.8613 (3)	0.29308 (8)	0.0574 (7)
H16	0.746563	0.798987	0.307775	0.069*
C18	0.6333 (2)	0.9799 (3)	0.22753 (8)	0.0597 (7)
C20	0.5313 (2)	1.0207 (3)	0.29215 (8)	0.0615 (7)
H20	0.471444	1.067128	0.306189	0.074*
C17	0.7088 (2)	0.8877 (3)	0.24971 (8)	0.0654 (8)
H17	0.769099	0.843147	0.235584	0.079*
C19	0.5450 (2)	1.0475 (4)	0.24884 (8)	0.0723 (9)
H19	0.494359	1.111272	0.234017	0.087*
H2	0.4564 (18)	0.832 (3)	0.4332 (7)	0.042 (6)*
H22A	0.317 (3)	0.729 (5)	0.3380 (11)	0.109 (14)*
H22B	0.364 (3)	0.867 (4)	0.3581 (10)	0.106 (12)*
H14	0.533 (2)	0.072 (4)	0.4599 (9)	0.082 (10)*
H21	0.705 (3)	0.949 (5)	0.1719 (12)	0.130 (14)*

Atomic displacement parameters (Å²) top

	U¹¹	U²²	U³³	U¹²	U¹³	U²³
O12	0.0429 (8)	0.0318 (7)	0.0375 (8)	−0.0015 (6)	−0.0074 (6)	0.0036 (6)
O14	0.0619 (10)	0.0250 (8)	0.0429 (9)	−0.0082 (7)	−0.0049 (8)	0.0019 (7)
N2	0.0417 (10)	0.0297 (9)	0.0329 (9)	0.0003 (8)	−0.0026 (8)	0.0031 (8)
N1	0.0431 (10)	0.0380 (10)	0.0340 (9)	0.0041 (8)	−0.0045 (8)	0.0021 (8)
O22	0.0638 (13)	0.0794 (14)	0.0599 (12)	0.0010 (11)	−0.0157 (10)	−0.0056 (11)
O21	0.0997 (17)	0.0965 (16)	0.0446 (11)	0.0282 (13)	0.0183 (11)	0.0222 (11)
C8	0.0293 (10)	0.0280 (10)	0.0289 (10)	0.0025 (8)	0.0020 (8)	0.0011 (8)
C9	0.0316 (10)	0.0238 (9)	0.0365 (11)	0.0002 (8)	0.0038 (9)	0.0004 (8)
C7	0.0300 (10)	0.0264 (10)	0.0331 (11)	−0.0013 (8)	−0.0015 (8)	−0.0024 (8)
C6	0.0329 (10)	0.0271 (10)	0.0336 (11)	−0.0006 (8)	0.0003 (8)	0.0001 (9)
C11	0.0368 (11)	0.0342 (11)	0.0329 (11)	0.0002 (9)	−0.0044 (9)	0.0028 (9)
C3	0.0360 (11)	0.0297 (10)	0.0387 (12)	−0.0042 (8)	−0.0078 (9)	0.0049 (9)
C10	0.0400 (11)	0.0308 (11)	0.0353 (11)	−0.0049 (9)	−0.0016 (9)	−0.0054 (9)
C5	0.0411 (12)	0.0316 (11)	0.0402 (12)	−0.0015 (9)	−0.0009 (10)	0.0040 (9)
C15	0.0464 (13)	0.0430 (12)	0.0400 (12)	0.0030 (10)	0.0018 (10)	0.0069 (10)
C4	0.0384 (12)	0.0435 (12)	0.0531 (14)	−0.0057 (9)	−0.0008 (10)	0.0162 (11)
C13	0.0535 (14)	0.0481 (13)	0.0430 (13)	−0.0083 (11)	−0.0160 (11)	0.0040 (11)
C16	0.0575 (16)	0.0672 (17)	0.0476 (14)	0.0172 (13)	0.0071 (12)	0.0136 (13)
C18	0.0713 (18)	0.0653 (17)	0.0426 (14)	0.0109 (14)	0.0114 (13)	0.0133 (13)
C20	0.0671 (17)	0.0702 (18)	0.0473 (14)	0.0255 (14)	0.0129 (13)	0.0154 (13)
C17	0.0643 (17)	0.0809 (19)	0.0511 (15)	0.0235 (15)	0.0170 (13)	0.0109 (15)
C19	0.079 (2)	0.089 (2)	0.0490 (15)	0.0365 (17)	0.0091 (14)	0.0240 (15)

Geometric parameters (Å, º) top

O12—C8	1.376 (2)	C3—H3	0.9800
O12—C13	1.432 (2)	C3—C4	1.531 (3)
O14—C9	1.364 (2)	C10—H10	0.9300
O14—H14	0.91 (3)	C5—C15	1.462 (3)
N2—N1	1.417 (2)	C5—C4	1.498 (3)
N2—C3	1.496 (3)	C15—C16	1.385 (3)
N2—H2	0.87 (2)	C15—C20	1.396 (3)
N1—C5	1.287 (3)	C4—H4A	0.9700
O22—H22A	0.91 (4)	C4—H4B	0.9700
O22—H22B	0.98 (4)	C13—H13A	0.9600
O21—C18	1.375 (3)	C13—H13B	0.9600
O21—H21	0.98 (4)	C13—H13C	0.9600
C8—C9	1.397 (3)	C16—H16	0.9300
C8—C7	1.383 (3)	C16—C17	1.384 (3)
C9—C10	1.386 (3)	C18—C17	1.375 (3)
C7—H7	0.9300	C18—C19	1.379 (3)
C7—C6	1.393 (3)	C20—H20	0.9300
C6—C11	1.387 (3)	C20—C19	1.382 (3)
C6—C3	1.513 (3)	C17—H17	0.9300
C11—H11	0.9300	C19—H19	0.9300
C11—C10	1.389 (3)

C8—O12—C13	117.18 (15)	N1—C5—C4	112.78 (18)
C9—O14—H14	108.7 (18)	C15—C5—C4	124.46 (19)
N1—N2—C3	109.04 (16)	C16—C15—C5	120.5 (2)
N1—N2—H2	106.6 (14)	C16—C15—C20	117.4 (2)
C3—N2—H2	113.6 (14)	C20—C15—C5	122.1 (2)
C5—N1—N2	109.81 (17)	C3—C4—H4A	111.1
H22A—O22—H22B	97 (3)	C3—C4—H4B	111.1
C18—O21—H21	112 (2)	C5—C4—C3	103.23 (17)
O12—C8—C9	115.37 (16)	C5—C4—H4A	111.1
O12—C8—C7	124.70 (17)	C5—C4—H4B	111.1
C7—C8—C9	119.93 (17)	H4A—C4—H4B	109.1
O14—C9—C8	116.60 (17)	O12—C13—H13A	109.5
O14—C9—C10	124.02 (18)	O12—C13—H13B	109.5
C10—C9—C8	119.36 (17)	O12—C13—H13C	109.5
C8—C7—H7	119.5	H13A—C13—H13B	109.5
C8—C7—C6	120.92 (18)	H13A—C13—H13C	109.5
C6—C7—H7	119.5	H13B—C13—H13C	109.5
C7—C6—C3	118.49 (17)	C15—C16—H16	119.2
C11—C6—C7	118.83 (17)	C17—C16—C15	121.7 (2)
C11—C6—C3	122.68 (17)	C17—C16—H16	119.2
C6—C11—H11	119.7	O21—C18—C17	122.4 (2)
C6—C11—C10	120.59 (18)	O21—C18—C19	118.0 (2)
C10—C11—H11	119.7	C17—C18—C19	119.7 (2)
N2—C3—C6	113.52 (16)	C15—C20—H20	119.5
N2—C3—H3	108.6	C19—C20—C15	121.0 (2)
N2—C3—C4	101.83 (15)	C19—C20—H20	119.5
C6—C3—H3	108.6	C16—C17—H17	120.0
C6—C3—C4	115.28 (17)	C18—C17—C16	119.9 (2)
C4—C3—H3	108.6	C18—C17—H17	120.0
C9—C10—C11	120.36 (18)	C18—C19—C20	120.3 (2)
C9—C10—H10	119.8	C18—C19—H19	119.9
C11—C10—H10	119.8	C20—C19—H19	119.9
N1—C5—C15	122.76 (19)

O12—C8—C9—O14	2.2 (2)	C7—C6—C3—N2	−121.91 (19)
O12—C8—C9—C10	−179.16 (17)	C7—C6—C3—C4	121.2 (2)
O12—C8—C7—C6	180.00 (17)	C6—C11—C10—C9	0.2 (3)
O14—C9—C10—C11	177.47 (18)	C6—C3—C4—C5	108.50 (19)
N2—N1—C5—C15	−174.82 (18)	C11—C6—C3—N2	57.3 (3)
N2—N1—C5—C4	4.4 (2)	C11—C6—C3—C4	−59.6 (3)
N2—C3—C4—C5	−14.9 (2)	C3—N2—N1—C5	−15.0 (2)
N1—N2—C3—C6	−106.21 (19)	C3—C6—C11—C10	−178.59 (18)
N1—N2—C3—C4	18.3 (2)	C5—C15—C16—C17	178.3 (2)
N1—C5—C15—C16	−163.9 (2)	C5—C15—C20—C19	−178.3 (3)
N1—C5—C15—C20	15.7 (3)	C15—C5—C4—C3	−173.42 (19)
N1—C5—C4—C3	7.3 (2)	C15—C16—C17—C18	0.2 (4)
O21—C18—C17—C16	−179.5 (3)	C15—C20—C19—C18	−0.1 (5)
O21—C18—C19—C20	179.4 (3)	C4—C5—C15—C16	17.0 (3)
C8—C9—C10—C11	−1.1 (3)	C4—C5—C15—C20	−163.5 (2)
C8—C7—C6—C11	−0.6 (3)	C13—O12—C8—C9	173.54 (18)
C8—C7—C6—C3	178.69 (17)	C13—O12—C8—C7	−6.8 (3)
C9—C8—C7—C6	−0.3 (3)	C16—C15—C20—C19	1.2 (4)
C7—C8—C9—O14	−177.51 (17)	C20—C15—C16—C17	−1.3 (4)
C7—C8—C9—C10	1.1 (3)	C17—C18—C19—C20	−1.0 (5)
C7—C6—C11—C10	0.6 (3)	C19—C18—C17—C16	0.9 (5)

Hydrogen-bond geometry (Å, º) top

D—H···A	D—H	H···A	D···A	D—H···A
N2—H2···O12ⁱ	0.87 (2)	2.36 (2)	3.209 (2)	167 (2)
O14—H14···N2ⁱⁱ	0.91 (3)	1.89 (3)	2.760 (2)	158 (3)
O21—H21···O22ⁱⁱⁱ	0.98 (4)	1.66 (4)	2.633 (3)	169 (4)
O22—H22A···O21^iv	0.91 (4)	1.99 (4)	2.891 (3)	171 (3)
O22—H22B···N1	0.98 (4)	1.84 (4)	2.794 (3)	166 (3)
C7—H7···O12^v	0.93	2.56	3.465 (2)	165

Symmetry codes: (i) −x+1, −y+1, −z+1; (ii) x, y−1, z; (iii) x+1/2, y, −z+1/2; (iv) −x+1, y−1/2, −z+1/2; (v) −x+3/2, y+1/2, z.

Enrichment ratios for 2 top

Parameter	Ratio
H···H	0.89
C···H	1.18
O···H	1.39
N···H	1.41
C···C	1.02

Acknowledgements

LVM thanks the Hercules Foundation for supporting the purchase of the diffractometer through project AKUL/09/ 0035.

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