Crystal structure, Hirshfeld surface analysis and inter­action energy, DFT and anti­bacterial activity studies of ethyl 2-[(2Z)-2-(2-chloro­benzyl­idene)-3-oxo-3,4-di­hydro-2H-1,4-benzo­thia­zin-4-yl]acetate

Sebbar, G.; Mohamed, E.; Hökelek, T.; Mague, J.T.; Sebbar, N.K.; Essassi, E.M.; Belkadi, B.

doi:10.1107/S2056989020004119

research communications

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ISSN: 2056-9890

Volume 76| Part 5| May 2020| Pages 629-636

https://doi.org/10.1107/S2056989020004119

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Crystal structure, Hirshfeld surface analysis and interaction energy, DFT and antibacterial activity studies of ethyl 2-[(2Z)-2-(2-chlorobenzylidene)-3-oxo-3,4-dihydro-2H-1,4-benzothiazin-4-yl]acetate

Ghizlane Sebbar,^a ^* Ellouz Mohamed,^b Tuncer Hökelek,^c Joel T. Mague,^d Nada Kheira Sebbar,^b,^e El Mokhtar Essassi ^b and Bouchra Belkadi ^a

^aLaboratory of Microbiology and Molecular Biology, Faculty of Sciences, University Mohammed V, Rabat, Morocco, ^bLaboratoire de Chimie Organique Heterocyclique URAC 21, Pole de Competence Pharmacochimie, Faculté des Sciences, Université Mohammed V, Rabat, Morocco, ^cDepartment of Physics, Hacettepe University, 06800 Beytepe, Ankara, Turkey, ^dDepartment of Chemistry, Tulane University, New Orleans, LA 70118, USA, and ^eLaboratoire de Chimie Appliquee et Environnement, Equipe de Chimie Bioorganique Appliquee, Faculte des Sciences, Université Ibn Zohr, Agadir, Morocco
^*Correspondence e-mail: sebbar.ghizlane@um5s.net.ma

Edited by A. J. Lough, University of Toronto, Canada (Received 5 March 2020; accepted 24 March 2020; online 7 April 2020)

The title compound, C₁₉H₁₆ClNO₃S, consists of chlorophenyl methylidene and dihydrobenzothiazine units linked to an acetate moiety, where the thiazine ring adopts a screw-boat conformation. In the crystal, two sets of weak C—H_Ph⋯O_Dbt (Ph = phenyl and Dbt = dihydrobenzothiazine) hydrogen bonds form layers of molecules parallel to the bc plane. The layers stack along the a-axis direction with intercalation of the ester chains. The crystal studied was a two component twin with a refined BASF of 0.34961 (5). The Hirshfeld surface analysis of the crystal structure indicates that the most important contributions to the crystal packing are from H⋯H (37.5%), H⋯C/C⋯H (24.6%) and H⋯O/O⋯H (16.7%) interactions. Hydrogen-bonding and van der Waals interactions are the dominant interactions in the crystal packing. Computational chemistry indicates that in the crystal, C—H_Ph⋯O_Dbt hydrogen bond energies are 38.3 and 30.3 kJ mol⁻¹. Density functional theory (DFT) optimized structures at the B3LYP/ 6–311 G(d,p) level are compared with the experimentally determined molecular structure in the solid state. The HOMO–LUMO behaviour was elucidated to determine the energy gap. Moreover, the antibacterial activity of the title compound has been evaluated against gram-positive and gram-negative bacteria.

Keywords: crystal structure; hydrogen bond; dihydrobenzothiazine; antibacterial activity; Hirshfeld surface.

CCDC reference: 1992626

1. Chemical context

A number of pharmacological tests have revealed 1,4-benzothiazine derivatives to possess a wide spectrum of biological applications, indicating that the 1,4-benzothiazine moiety is a potentially useful template in medicinal chemistry research and therapeutic applications such as in vivo antiproliferative (Zięba et al., 2016 ), antibacterial (Sebbar et al., 2016b ; Ellouz et al., 2019 ), antimicrobial (Armenise et al., 2012 ; Sabatini et al., 2008 ; Vijay & Rahul, 2016 ), anti-viral (Malagu et al., 1998 ), anti-oxidant (Zia-ur-Rehman et al., 2009 ), anti-inflammatory (Trapani et al., 1985 ; Gowda et al., 2011 ), antipyretic (Warren & Knaus, 1987 ) and anti-cancer (Gupta & Gupta, 1991 ; Gupta et al., 1985 ) areas. They have also been reported as precursors for the syntheses of new compounds (Sebbar et al., 2015a ; Vidal et al., 2006 ) possessing anti-diabetic (Tawada et al., 1990 ) and anti-corrosion (Ellouz et al., 2016a ,b ) activities, and as antiproliferative (Zięba et al., 2010 ) or antihelmintic (Munirajasekar et al., 2011 ) agents. The biological activities of some 1,4-benzothiazines are similar to those of phenothiazines, featuring the same structural specificity (Hni et al., 2019a ,b ; Ellouz et al., 2017a , 2018 , 2019; Sebbar et al., 2019a ,b ). In a continuation of our research activities devoted to the development of N-substituted 1,4-benzothiazine derivatives and the evaluation of their potential pharmacological activities (Ellouz et al., 2017a; Sebbar et al., 2017a ), we have synthesized a new heterocyclic system containing 1,4-benzothiazine. We report herein the synthesis and the molecular and crystal structures along with the Hirshfeld surface analysis and interaction energy calculations [using the CE–B3LYP/6–31G(d,p) energy model] and the density functional theory (DFT) computational calculations carried out at the B3LYP/6–311 G(d,p) level compared with the experimentally determined molecular structure in the solid state. Moreover, the antibacterial activity of the title compound has been evaluated against gram-positive and gram-negative bacteria (e.g. Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa).

2. Structural commentary

The title compound, (I), consists of chlorophenyl methylidene and dihydrobenzothiazine units linked to an acetate moiety, where the thiazine ring adopts a screw-boat conformation (Fig. 1). The dihydrobenzothiazine ring is folded across the N1⋯S1 axis by 36.70 (7)°. A puckering analysis of the thiazine, B (N1/S1/C1/C6–C8), ring conformation gave the parameters Q_T = 0.5525 (16) Å, θ = 109.0 (2)° and φ = 161.0 (2)°, indicating a screw-boat conformation. The mean plane of the N1/C16/C17/O2/O3 group is inclined to the mean plane of the S1/C1–C6/N1 unit by 80.06 (7)° while the phenyl, C (C10–C15), ring makes a dihedral angle of 84.92 (6)° with the latter plane. The benzene ring A (C1–C6) is oriented at a dihedral angle of 84.46 (2)° with respect to the C ring.

Figure 1
The asymmetric unit of the title compound with the atom-numbering scheme. Displacement ellipsoids are drawn at the 50% probability level.

3. Supramolecular features

In the crystal, two sets of weak C—H_Ph⋯O_Dbt (Ph = phenyl and Dbt = dihydrobenzothiazine) hydrogen bonds (Table 1) form layers of molecules parallel to the bc plane (Fig. 2). The layers stack along the a-axis direction with intercalation of the ester chains (Fig. 2).

Table 1
Hydrogen-bond geometry (Å, °)

D—H⋯A	D—H	H⋯A	D⋯A	D—H⋯A
C12—H12⋯O1^vii	0.95 (3)	2.56 (3)	3.214 (2)	126 (2)
C15—H15⋯O1ⁱⁱ	0.95 (2)	2.40 (2)	3.227 (2)	145.8 (15)
Symmetry codes: (ii) -x+1, -y+1, -z+1; (vii) $[-x+1, y+{\script{1\over 2}}, -z+{\script{1\over 2}}]$ .

Figure 2
A partial packing diagram viewed along the b-axis direction. The weak C—H_Ph⋯O_Dbt (Ph = phenyl and Dbt = dihydrobenzothiazine) hydrogen bonds are depicted by black dashed lines.

4. Hirshfeld surface analysis

In order to visualize the intermolecular interactions in the crystal of the title compound, a Hirshfeld surface (HS) analysis (Hirshfeld, 1977 ; Spackman & Jayatilaka, 2009 ) was carried out using Crystal Explorer 17.5 (Turner et al., 2017 ). In the HS plotted over d_norm (Fig. 3), the white surface indicates contacts with distances equal to the sum of van der Waals radii, and the red and blue colours indicate distances shorter (in close contact) or longer (distant contact) than the van der Waals radii, respectively (Venkatesan et al., 2016 ). The bright-red spots appearing near O1 and hydrogen atom H15 indicate their roles as the respective donors and/or acceptors; they also appear as blue and red regions corresponding to positive and negative potentials on the HS mapped over electrostatic potential (Spackman et al., 2008 ; Jayatilaka et al., 2005 ) as shown in Fig. 4. Here the blue regions indicate positive electrostatic potential (hydrogen-bond donors), while the red regions indicate negative electrostatic potential (hydrogen-bond acceptors). The shape-index of the HS is a tool to visualize the π–π stacking by the presence of adjacent red and blue triangles; if there are no adjacent red and/or blue triangles, then there are no π–π interactions. Fig. 5 clearly suggests that there are no π–π interactions in (I).

Figure 3
View of the three-dimensional Hirshfeld surface of the title compound plotted over d_norm in the range −0.1956 to 1.3971 a.u.

Figure 4
View of the three-dimensional Hirshfeld surface of the title compound plotted over electrostatic potential energy in the range −0.0500 to 0.0500 a.u. using the STO-3 G basis set at the Hartree–Fock level of theory. Hydrogen-bond donors and acceptors are shown as blue and red regions around the atoms corresponding to positive and negative potentials, respectively.

Figure 5
Hirshfeld surface of the title compound plotted over shape-index.

The overall two-dimensional fingerprint plot, Fig. 6a, and those delineated into H⋯H, H⋯C/C⋯H, H⋯O/O⋯H, H⋯Cl/Cl⋯H, C⋯Cl/Cl⋯C, H⋯S/S⋯H, S⋯Cl/Cl⋯S and C⋯C contacts (McKinnon et al., 2007 ) are illustrated in Fig. 6b–i, respectively, together with their relative contributions to the Hirshfeld surface. The most important interaction is H⋯H (Table 2) contributing 37.5% to the overall crystal packing, which is reflected in Fig. 6b as widely scattered points of high density due to the large hydrogen-atom content of the molecule with the tip at d_e = d_i = 1.10 Å. The pair of characteristic wings in the fingerprint plot delineated into H⋯C/C⋯H contacts (Table 2, Fig. 6c; 24.6% contribution to the HS), have tips at d_e + d_i = 2.72 Å. The H⋯O/O⋯H contacts (Table 1, Fig. 6d) with a 16.7% contribution to the HS have a symmetric distribution of points with the tips at d_e + d_i = 2.27 Å. The scattered points in the wings in the fingerprint plot delineated into H⋯Cl/Cl⋯H, Fig. 6e, contacts (7.1% contribution) have the tips at d_e + d_i = 3.14 Å. The C⋯Cl/Cl⋯C contacts, Fig. 6f, with 4.2% contribution to the HS have an arrow-shaped distribution of points of split small wings with the tips at d_e + d_i = 3.41 Å. The pair of spikes in the fingerprint plot delineated into H⋯S/S⋯H, Fig. 6g, contacts (4.0% contribution) have tips at d_e + d_i = 2.78 Å. The pair of characteristic wings in the fingerprint plot delineated into S⋯Cl/Cl⋯S contacts, Fig. 6h, (2.1% contribution) has the tips at d_e + d_i = 3.70 Å. Finally, the C⋯C contacts, Fig. 6i, (1.3% contribution) have an arrow-shaped distribution of points with the tip at d_e = d_i = 1.85 Å.

Table 2
Selected interatomic distances (Å)

Cl1⋯C14ⁱ	3.5363 (9)	O1⋯H12ⁱ	2.560 (9)
Cl1⋯S1ⁱ	3.7268 (3)	O1⋯H15ⁱⁱ	2.400 (8)
Cl1⋯C10ⁱ	3.5940 (7)	O2⋯H18B	2.545 (11)
Cl1⋯C15ⁱ	3.4359 (7)	O2⋯H13ⁱ	2.606 (10)
Cl1⋯H9	2.674 (8)	O2⋯H18A	2.74 (3)
S1⋯N1	3.0100 (6)	O3⋯H16B^vi	2.666 (8)
S1⋯C15	3.1748 (8)	C2⋯C17	3.2932 (10)
S1⋯O1ⁱⁱ	3.4189 (6)	C4⋯C14ⁱⁱⁱ	3.5882 (12)
S1⋯H15	2.660 (10)	C2⋯H16B	2.621 (8)
S1⋯H5ⁱⁱⁱ	2.906 (9)	C4⋯H14ⁱⁱⁱ	2.826 (10)
O1⋯C17	3.1263 (9)	C4⋯H12^vii	2.993 (10)
O1⋯C12ⁱ	3.2141 (10)	C5⋯H12^vii	2.817 (10)
O1⋯C15ⁱⁱ	3.2268 (8)	C7⋯H15	2.937 (9)
O2⋯N1	2.7902 (7)	C15⋯H16Aⁱⁱ	2.900 (9)
O2⋯C8	3.2091 (9)	C16⋯H16B^vi	2.987 (9)
O2⋯C1	3.3715 (8)	C16⋯H2	2.538 (9)
O2⋯C3^iv	3.3498 (10)	C17⋯H2	2.696 (9)
O2⋯C2	3.4103 (9)	H2⋯H16B	2.223 (12)
O1⋯H9	2.516 (9)	H5⋯H12^vii	2.444 (13)
O1⋯H16A	2.376 (9)	H5⋯H15ⁱⁱⁱ	2.509 (12)
O1⋯H4^v	2.806 (11)	H16B⋯H16B^vi	2.381 (13)
Symmetry codes: (i) $[-x+1, y-{\script{1\over 2}}, -z+{\script{1\over 2}}]$ ; (ii) -x+1, -y+1, -z+1; (iii) -x+1, -y+2, -z+1; (iv) $[-x, y-{\script{1\over 2}}, -z+{\script{1\over 2}}]$ ; (v) x, y-1, z; (vi) -x, -y+1, -z+1; (vii) $[-x+1, y+{\script{1\over 2}}, -z+{\script{1\over 2}}]$ .

Figure 6
The full two-dimensional fingerprint plots for the title compound, showing (a) all interactions, and delineated into (b) H⋯H, (c) H⋯C/C⋯H, (d) H⋯O/O⋯H, (e) H⋯Cl/Cl ⋯ H, (f) C⋯Cl/Cl⋯C, (g) H⋯S/S⋯H, (h) S ⋯ Cl/Cl⋯S and (i) C⋯C interactions. The d_i and d_e values are the closest internal and external distances (in Å) from given points on the Hirshfeld surface.

The Hirshfeld surface representations with the function d_norm plotted onto the surface are shown for the H⋯H, H⋯C/C⋯H, H⋯O/O⋯H and H⋯Cl/Cl⋯H interactions in Fig. 7a-d, respectively.

Figure 7
Hirshfeld surface representations with the function d_norm plotted onto the surface for (a) H⋯H, (b) H⋯C/C⋯H, (c) H⋯O/O⋯H and (d) H⋯Cl/Cl⋯H interactions.

The Hirshfeld surface analysis confirms the importance of H-atom contacts in establishing the packing. The large number of H⋯H, H⋯C/C⋯H and H⋯O/O⋯H interactions suggest that van der Waals interactions and hydrogen bonding play the major roles in the crystal packing (Hathwar et al., 2015 ).

5. Interaction energy calculations

The intermolecular interaction energies were calculated using the CE–B3LYP/6–31G(d,p) energy model available in Crystal Explorer 17.5 (Turner et al., 2017), where a cluster of molecules is generated by applying crystallographic symmetry operations with respect to a selected central molecule within a default radius of 3.8 Å (Turner et al., 2014 ). The total intermolecular energy (E_tot) is the sum of electrostatic (E_ele), polarization (E_pol), dispersion (E_dis) and exchange-repulsion (E_rep) energies (Turner et al., 2015 ) with scale factors of 1.057, 0.740, 0.871 and 0.618, respectively (Mackenzie et al., 2017 ). Hydrogen-bonding interaction energies (in kJ mol⁻¹) were calculated to be −20.3 (E_ele), −5.9 (E_pol), −48.7 (E_dis), 48.5 (E_rep) and −38.3 (E_tot) for C15—H15⋯O1 and −15.2 (E_ele), −4.1 (E_pol), −42.2 (E_dis), 41.3 (E_rep) and −30.3 (E_tot) for C12—H12⋯O1.

6. DFT calculations

The optimized structure of the title compound, (I), in the gas phase was generated theoretically via density functional theory (DFT) using the standard B3LYP functional and 6–311 G(d,p) basis-set calculations (Becke, 1993 ) as implemented in GAUSSIAN 09 (Frisch et al., 2009 ). The theoretical and experimental results are in good agreement (Table 3). The highest-occupied molecular orbital (HOMO), acting as an electron donor, and the lowest-unoccupied molecular orbital (LUMO), acting as an electron acceptor, are important parameters for quantum chemistry. When the energy gap is small, the molecule is highly polarizable and has high chemical reactivity. The DFT calculations provide some important information on the reactivity and site selectivity of the molecular framework. E_HOMO and E_LUMO clarify the inevitable charge-exchange collaboration inside the studied material, electronegativity (χ), hardness (η), potential (μ), electrophilicity (ω) and softness (σ) are recorded in Table 4. The parameters η and σ are significant for the evaluation of both the reactivity and stability. The electron transition from the HOMO to the LUMO energy level is shown in Fig. 8. The HOMO and LUMO are localized in the plane extending from the whole 2-[(2Z)-2-(2-chlorobenzylidene)-3-oxo-3,4-dihydro-2H-1,4-benzothiazin-4-yl]acetate ring. The energy band gap [ΔE = E_LUMO - E_HOMO] of the molecule is 4.3346 eV, and the frontier molecular orbital energies, E_HOMO and E_LUMO are −5.2696 and −0.9347 eV, respectively.

Table 3
Comparison of selected (X-ray and DFT) geometric data (Å, °)

Bonds/angles	X-ray	B3LYP/6–311G(d,p)
Cl1—C11	1.741 (2)	1.83593
S1—C6	1.755 (2)	1.83362
S1—C7	1.757 (2)	1.79349
O1—C8	1.224 (2)	1.26839
O2—C17	1.200 (2)	1.23993
O3—C17	1.335 (2)	1.36867
O3—C18	1.462 (3)	1.48321
N1—C8	1.381 (2)	1.40044
N1—C1	1.417 (2)	1.41683
N1—C16	1.452 (2)	1.47008

C6—S1—C7	98.19 (9)	99.41730
C17—O3—C18	116.60 (16)	116.97676
C8—N1—C1	124.52 (15)	125.49531
C8—N1—C16	115.56 (16)	115.02066
C1—N1—C16	118.47 (16)	118.38057
C2—C1—N1	121.41 (17)	121.23845
C2—C1—C6	118.60 (18)	117.94010
C6—C1—N1	120.00 (17)	120.81444
O1—C8—N1	120.36 (17)	120.12402
O1—C8—C7	121.99 (17)	120.12402
N1—C8—C7	117.64 (16)	117.79908

Table 4
Calculated energies

Molecular Energy (a.u.) (eV)	Compound (I)
Total Energy, TE (eV)	−50964
E_HOMO (eV)	−5.2696
E_LUMO (eV)	−0.9347
Gap, ΔE (eV)	4.3346
Dipole moment, μ (Debye)	5.6841
Ionization potential, I (eV)	5.2696
Electron affinity, A	0.9347
Electronegativity, χ	3.1019
Hardness, η	2.1673
Electrophilicity index, ω	2.2198
Softness, σ	0.4614
Fraction of electron transferred, ΔN	0.8993

Figure 8
The energy band gap of the title compound, (I)

7. Database survey

A search of the Cambridge Structural Database (Version 5.38; Groom et al., 2016 ) with the fragment (II) yielded 16 hits. The largest group is that for (III) with R = Ph and R′ = A (WUFGIP; Sebbar et al., 2015b ), CH₂COOH (APAJUY; Sebbar et al., 2016a ), (CH₂)₁₇CH₃ (CARCEG; Sebbar et al., 2017a), n-Bu (JOGVOS; Sebbar et al., 2014a ), CH₂C≡CH (COGRUN; Sebbar et al., 2014b ), R = Ph and R′ = B (EVIYIT; (Sebbar et al., 2016b), CH₂COOCH₃ (ICAJOL; Zerzouf et al., 2001 ), R = Ph and R′ = C (JADPOW; Ellouz et al., 2015 ) and R = Ph and R′ = D (OBITUR; Sebbar et al., 2016c ). The remainder have R = 4-ClC₆H₄ and R′ = bz (OMEGEU; Ellouz et al., 2016c ), n-Bu (PAWCIC; Ellouz et al., 2017a) and R = 4-ClC₆H₄ and R′ = B (YANHAZ; Ellouz et al., 2017b ) or R = 2-ClC₆H₄, and R′ = CH₂C≡CH (SAVTUH; Sebbar et al., 2017b ) or R = 4-FC₆H₄ and R′ = CH₂C≡CH (WOCFUS; Hni et al., 2019a) or R = 2,4-Cl₂C₆H₃ and R′ = B (DOHZUY; Hni et al., 2019b, CH₂CH₂CN (POHPOU; Sebbar et al., 2019a). In the majority of these, the thiazine ring is significantly folded about the S⋯N axis with dihedral angles between the two S/C/C/N planes ranging from ca 35° (JADPOW and WUFGIP) to ca 27° (COGRUN and WOCFUS). Two others have intermediate values of ca 15° (POHPOU) and 9° (DOHZUY), while in the last three, the thiazine ring is nearly flat with a dihedral angle of ca 4° (EVIYIT, OBITUR and OMEGEU). It is not immediately obvious what the reasons are for these nearly planar rings, but it may be in part due to packing considerations since in these last three molecules, the substituents on the thiazine rings do not hold the benzothiazine moieties as far apart as in the other cases, so that π-stacking interactions between the benzo portions can bring them close together and flatten out the rings.

8. Antibacterial activity

To compare and analyse the antibacterial behaviour contributed by (I), and commercial antibiotics such as Chloramphenicol (Chlor) and Ampicillin (Amp), we have tested the title compound, (I), against Staphylococcus aureus (ATCC-25923), Escherichia coli (ATTC-25922) and Pseudomonas aeruginosa (ATCC-27853) strains of bacteria using the diffusion disk method to evaluate the applicability of (I) as an antibacterial agent (Mabkhot et al., 2016 ; Hoffmann et al., 2017 ). Fig. 9 summarizes the diameter of inhibition (mm) values of (I) and commercial antibiotics chloramphenicol (Chlor) and ampicillin (Amp) against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. The determination of the minimum inhibition concentration (MIC) values of the sample (I) against the bacteria are presented in Table 5. The results of antibacterial activity of the product tested showed the best activity with MIC value of 21 µg mL⁻¹ and different degrees of growth inhibition against the bacteria tested. It is clear that there is a significant enhancement and a strong antibacterial activity associated with sample (I), as compared to commercial antibiotics. In addition, the maximum effect of (I) was recorded against Staphylococcus aureus (diameter of inhibition 16.4 mm). Chloramphenicol and ampicillin present a moderate antibacterial activity diameter of inhibition 22.6 mm and 11.75 mm, respectively, and no zone inhibition was observed with DMSO. On one hand, the chemical structure of (I) can explain this biologic effect. The mechanism of action of (I) is not attributable to one specific mechanism, but there are several targets in the cell: degradation of the cell wall, damage to membrane proteins, damage to cytoplasmic membrane, leakage of cell contents and coagulation of cytoplasm. On the other hand, it should be noted that the derivatives functionalized by ester groups and benzene rings have the highest antibacterial coefficient (92% of pathogenic bacteria are sensitive). This study is expected to include anti-inflammatory, antifungal, anti-parasitic and anti-cancer activities, because the literature gives a lot of interesting results on these topics. Some other types of bacteria may possibly be tested by employing the same method so as to eventually generalize the suggested investigation method (Alderman & Smith, 2001 ).

Table 5
Minimal inhibitory concentration [MIC (μg mL⁻¹)]

ATCC-25923 = Staphylococcus aureus, ATTC-25922 = Escherichia coli, ATCC-27853 = Pseudomonas aeruginosa, Chlor = chloramphenicol and Amp = ampicillin.

Product	ATCC-25923	ATTC-25922	ATCC-27853
(I)	21	21	21
Chlor	58	58	58
Amp	12	12	12
DMSO	0	0	0

Figure 9
Antibacterial activity of the title compound, (I)

, and the commercial antibiotics chloramphenicol (Chlor) and ampicillin (Amp) against the bacteria Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa.

9. Synthesis and crystallization

To a solution of 2-(2-chlorobenzylidene)-3,4-dihydro-2H-1,4-benzothiazin-3-one (0.57 g, 2 mmol), potassium carbonate (4 mmol) and tetra n-butyl ammonium bromide (0.2 mmol) in DMF (14 ml) was added ethyl chloroacetate (0.49 g, 4 mmol). Stirring was continued at room temperature for 14 h. The mixture was filtered and the solvent removed. The residue was extracted with water. The organic compound was chromatographed on a column of silica gel with ethyl acetate–hexane (8:2) as eluent. Colourless crystals of the title compound, (I), were isolated when the solvent was allowed to evaporate (yield: 66%).

10. Refinement

Crystal data, data collection and structure refinement details are summarized in Table 6. Hydrogen atoms were located in a difference-Fourier map and refined freely. The model was refined as a two-component twin with twin law $[\overline{1}]$ 0 0, 0 $[\overline{1}]$ 0, 0 0 $[\overline{1}]$ and a refined BASF parameter of 0.34961 (5).

Table 6
Experimental details

Crystal data
Chemical formula	C₁₉H₁₆ClNO₃S
M_r	373.84
Crystal system, space group	Monoclinic, P2₁/c
Temperature (K)	150
a, b, c (Å)	11.6882 (2), 9.0903 (2), 16.9533 (3)
β (°)	105.105 (1)
V (Å³)	1739.04 (6)
Z	4
Radiation type	Cu Kα
μ (mm⁻¹)	3.22
Crystal size (mm)	0.19 × 0.15 × 0.11

Data collection
Diffractometer	Bruker D8 VENTURE PHOTON 100 CMOS
Absorption correction	Multi-scan (TWINABS; Sheldrick, 2009 )
T_min, T_max	0.57, 0.72
No. of measured, independent and observed [I > 2σ(I)] reflections	25761, 25761, 21950
R_int	0.032
(sin θ/λ)_max (Å⁻¹)	0.625

Refinement
R[F² > 2σ(F²)], wR(F²), S	0.039, 0.101, 1.03
No. of reflections	25761
No. of parameters	292
H-atom treatment	All H-atom parameters refined
Δρ_max, Δρ_min (e Å⁻³)	0.72, −0.80
Computer programs: APEX3 and SAINT (Bruker, 2016 ), CELL_NOW (Sheldrick, 2008a ), SHELXT (Sheldrick, 2015a ), SHELXL2018 (Sheldrick, 2015b ), DIAMOND (Brandenburg & Putz, 2012 ) and SHELXTL (Sheldrick, 2008b ).

Supporting information

CCDC reference: 1992626

Crystal structure: contains datablocks I, global. DOI: https://doi.org/10.1107/S2056989020004119/lh5950sup1.cif

Structure factors: contains datablock I. DOI: https://doi.org/10.1107/S2056989020004119/lh5950Isup2.hkl

Supporting information file. DOI: https://doi.org/10.1107/S2056989020004119/lh5950Isup3.cdx

Supporting information file. DOI: https://doi.org/10.1107/S2056989020004119/lh5950Isup4.cml

checkCIF report

Computing details top

Data collection: APEX3 (Bruker, 2016); cell refinement: SAINT (Bruker, 2016); data reduction: SAINT (Bruker, 2016), CELL_NOW (Sheldrick, 2008a); program(s) used to solve structure: SHELXT (Sheldrick, 2015a); program(s) used to refine structure: SHELXL2018 (Sheldrick, 2015b); molecular graphics: DIAMOND (Brandenburg & Putz, 2012); software used to prepare material for publication: SHELXTL (Sheldrick, 2008b).

Ethyl 2-[(2Z)-2-(2-chlorobenzylidene)-3-oxo-3,4-dihydro-2H-\ 1,4-benzothiazin-4-yl]acetate top

Crystal data top

C₁₉H₁₆ClNO₃S	F(000) = 776
M_r = 373.84	D_x = 1.428 Mg m⁻³
Monoclinic, P2₁/c	Cu Kα radiation, λ = 1.54178 Å
a = 11.6882 (2) Å	Cell parameters from 9820 reflections
b = 9.0903 (2) Å	θ = 3.9–74.6°
c = 16.9533 (3) Å	µ = 3.22 mm⁻¹
β = 105.105 (1)°	T = 150 K
V = 1739.04 (6) Å³	Block, colourless
Z = 4	0.19 × 0.15 × 0.11 mm

Data collection top

Bruker D8 VENTURE PHOTON 100 CMOS diffractometer	25761 independent reflections
Radiation source: INCOATEC IµS micro–focus source	21950 reflections with I > 2σ(I)
Mirror monochromator	R_int = 0.032
Detector resolution: 10.4167 pixels mm^-1	θ_max = 74.6°, θ_min = 3.9°
ω scans	h = −14→13
Absorption correction: multi-scan (TWINABS; Sheldrick, 2009)	k = −11→10
T_min = 0.57, T_max = 0.72	l = −21→21
25761 measured reflections

Refinement top

Refinement on F²	Secondary atom site location: difference Fourier map
Least-squares matrix: full	Hydrogen site location: difference Fourier map
R[F² > 2σ(F²)] = 0.039	All H-atom parameters refined
wR(F²) = 0.101	w = 1/[σ²(F_o²) + (0.0405P)² + 0.4043P] where P = (F_o² + 2F_c²)/3
S = 1.02	(Δ/σ)_max < 0.001
25761 reflections	Δρ_max = 0.72 e Å⁻³
292 parameters	Δρ_min = −0.80 e Å⁻³
0 restraints	Extinction correction: SHELXL2018/1 (Sheldrick, 2015b), Fc^*=kFc[1+0.001xFc²λ³/sin(2θ)]^-1/4
Primary atom site location: dual space	Extinction coefficient: 0.0032 (6)

Special details top

Experimental. Analysis of 529 reflections having I/σ(I) > 12 and chosen from the full data set with CELL_NOW (Sheldrick, 2008a) showed the crystal to belong to the monoclinic system and to be twinned by a 180° rotation about the b axis. The raw data were processed using the multi- component version of SAINT under control of the two-component orientation file generated by CELL_NOW.

Geometry. All esds (except the esd in the dihedral angle between two l.s. planes) are estimated using the full covariance matrix. The cell esds are taken into account individually in the estimation of esds in distances, angles and torsion angles; correlations between esds in cell parameters are only used when they are defined by crystal symmetry. An approximate (isotropic) treatment of cell esds is used for estimating esds involving l.s. planes.

Refinement. Refinement of F² against ALL reflections. The weighted R-factor wR and goodness of fit S are based on F², conventional R-factors R are based on F, with F set to zero for negative F². The threshold expression of F² > 2sigma(F²) is used only for calculating R-factors(gt) etc. and is not relevant to the choice of reflections for refinement. R-factors based on F² are statistically about twice as large as those based on F, and R- factors based on ALL data will be even larger. Refined as a 2-component twin.

Fractional atomic coordinates and isotropic or equivalent isotropic displacement parameters (Å²) top

	x	y	z	U_iso*/U_eq
Cl1	0.50488 (5)	0.51497 (7)	0.19568 (3)	0.03952 (18)
S1	0.44083 (4)	0.78070 (6)	0.47131 (3)	0.02735 (16)
O1	0.31103 (13)	0.39288 (15)	0.41536 (8)	0.0264 (3)
O2	0.05392 (14)	0.49512 (17)	0.29883 (9)	0.0341 (4)
O3	−0.04896 (13)	0.37965 (16)	0.37586 (9)	0.0306 (3)
N1	0.21745 (14)	0.60019 (17)	0.43805 (10)	0.0223 (3)
C1	0.19875 (18)	0.7541 (2)	0.42964 (11)	0.0216 (4)
C2	0.08488 (19)	0.8137 (2)	0.40863 (13)	0.0272 (4)
H2	0.019 (2)	0.750 (3)	0.3964 (15)	0.032 (6)*
C3	0.0683 (2)	0.9646 (2)	0.40245 (13)	0.0299 (5)
H3	−0.010 (3)	1.002 (3)	0.3866 (16)	0.038 (7)*
C4	0.1643 (2)	1.0587 (2)	0.41608 (13)	0.0294 (5)
H4	0.152 (3)	1.159 (3)	0.4110 (17)	0.045 (7)*
C5	0.2778 (2)	1.0009 (2)	0.43568 (13)	0.0274 (4)
H5	0.345 (2)	1.066 (3)	0.4429 (15)	0.033 (6)*
C6	0.29556 (17)	0.8496 (2)	0.44327 (11)	0.0228 (4)
C7	0.41573 (18)	0.6168 (2)	0.41447 (11)	0.0224 (4)
C8	0.31254 (18)	0.5270 (2)	0.42214 (11)	0.0214 (4)
C9	0.48358 (18)	0.5650 (2)	0.36782 (12)	0.0239 (4)
H9	0.461 (2)	0.474 (3)	0.3411 (15)	0.030 (6)*
C10	0.58341 (18)	0.6384 (2)	0.34694 (12)	0.0230 (4)
C11	0.59934 (19)	0.6260 (2)	0.26787 (12)	0.0266 (4)
C12	0.6869 (2)	0.7022 (2)	0.24392 (13)	0.0308 (5)
H12	0.693 (2)	0.689 (3)	0.1896 (16)	0.039 (7)*
C13	0.7644 (2)	0.7914 (2)	0.29922 (13)	0.0316 (5)
H13	0.826 (3)	0.842 (3)	0.2818 (17)	0.042 (7)*
C14	0.75389 (19)	0.8019 (2)	0.37885 (13)	0.0281 (4)
H14	0.808 (2)	0.863 (3)	0.4187 (16)	0.037 (7)*
C15	0.66479 (18)	0.7271 (2)	0.40190 (12)	0.0255 (4)
H15	0.659 (2)	0.733 (2)	0.4564 (15)	0.027 (6)*
C16	0.11979 (18)	0.5077 (2)	0.44607 (12)	0.0235 (4)
H16A	0.151 (2)	0.421 (3)	0.4763 (15)	0.029 (6)*
H16B	0.077 (2)	0.556 (3)	0.4769 (14)	0.026 (6)*
C17	0.03970 (18)	0.4622 (2)	0.36420 (12)	0.0244 (4)
C18	−0.1348 (2)	0.3263 (3)	0.30254 (15)	0.0386 (5)
H18A	−0.092 (3)	0.255 (3)	0.2763 (18)	0.048 (8)*
H18B	−0.161 (3)	0.410 (3)	0.2641 (19)	0.049 (8)*
C19	−0.2350 (3)	0.2585 (4)	0.3297 (2)	0.0509 (7)
H19A	−0.205 (3)	0.173 (3)	0.370 (2)	0.054 (8)*
H19B	−0.289 (3)	0.220 (4)	0.283 (2)	0.069 (10)*
H19C	−0.274 (3)	0.331 (4)	0.359 (2)	0.061 (9)*

Atomic displacement parameters (Å²) top

	U¹¹	U²²	U³³	U¹²	U¹³	U²³
Cl1	0.0417 (4)	0.0478 (3)	0.0343 (3)	−0.0153 (2)	0.0194 (2)	−0.0140 (2)
S1	0.0165 (3)	0.0318 (3)	0.0341 (3)	−0.00303 (19)	0.0072 (2)	−0.0083 (2)
O1	0.0279 (8)	0.0251 (7)	0.0300 (7)	−0.0012 (6)	0.0143 (6)	0.0008 (5)
O2	0.0335 (9)	0.0442 (9)	0.0270 (7)	−0.0083 (7)	0.0122 (7)	0.0003 (6)
O3	0.0253 (8)	0.0361 (8)	0.0318 (7)	−0.0111 (6)	0.0100 (6)	−0.0034 (6)
N1	0.0180 (8)	0.0251 (8)	0.0265 (8)	−0.0032 (6)	0.0104 (7)	0.0002 (6)
C1	0.0196 (10)	0.0247 (9)	0.0226 (8)	−0.0025 (8)	0.0093 (8)	−0.0020 (7)
C2	0.0178 (10)	0.0308 (10)	0.0340 (10)	−0.0033 (8)	0.0086 (8)	−0.0031 (8)
C3	0.0215 (11)	0.0327 (11)	0.0357 (11)	0.0027 (9)	0.0080 (9)	−0.0027 (9)
C4	0.0296 (12)	0.0246 (10)	0.0357 (11)	0.0008 (9)	0.0117 (9)	−0.0024 (8)
C5	0.0238 (11)	0.0282 (10)	0.0323 (10)	−0.0061 (8)	0.0108 (9)	−0.0064 (8)
C6	0.0170 (10)	0.0287 (10)	0.0241 (9)	−0.0019 (8)	0.0083 (8)	−0.0036 (7)
C7	0.0190 (10)	0.0253 (9)	0.0236 (9)	−0.0012 (7)	0.0072 (8)	0.0016 (7)
C8	0.0200 (10)	0.0274 (10)	0.0184 (8)	−0.0010 (8)	0.0076 (7)	0.0006 (7)
C9	0.0217 (10)	0.0254 (10)	0.0262 (9)	−0.0004 (8)	0.0094 (8)	0.0020 (8)
C10	0.0191 (10)	0.0252 (9)	0.0275 (9)	0.0040 (7)	0.0111 (8)	0.0039 (7)
C11	0.0243 (11)	0.0282 (10)	0.0300 (10)	0.0001 (8)	0.0121 (9)	−0.0012 (8)
C12	0.0313 (12)	0.0368 (12)	0.0298 (10)	−0.0013 (9)	0.0177 (9)	0.0006 (9)
C13	0.0249 (11)	0.0386 (12)	0.0359 (11)	−0.0044 (9)	0.0164 (9)	0.0038 (9)
C14	0.0181 (10)	0.0374 (11)	0.0296 (10)	−0.0027 (9)	0.0072 (8)	0.0006 (9)
C15	0.0196 (10)	0.0333 (10)	0.0248 (9)	0.0022 (8)	0.0083 (8)	0.0038 (8)
C16	0.0208 (10)	0.0267 (10)	0.0268 (9)	−0.0039 (8)	0.0129 (8)	0.0003 (8)
C17	0.0212 (10)	0.0239 (9)	0.0307 (10)	−0.0017 (8)	0.0114 (8)	−0.0017 (8)
C18	0.0306 (13)	0.0435 (13)	0.0387 (12)	−0.0113 (11)	0.0033 (10)	−0.0085 (11)
C19	0.0319 (15)	0.0561 (17)	0.0614 (17)	−0.0187 (13)	0.0065 (14)	−0.0055 (15)

Geometric parameters (Å, º) top

Cl1—C11	1.741 (2)	C9—C10	1.465 (3)
S1—C6	1.755 (2)	C9—H9	0.95 (2)
S1—C7	1.757 (2)	C10—C15	1.401 (3)
O1—C8	1.224 (2)	C10—C11	1.405 (3)
O2—C17	1.200 (2)	C11—C12	1.382 (3)
O3—C17	1.335 (2)	C12—C13	1.383 (3)
O3—C18	1.462 (3)	C12—H12	0.95 (3)
N1—C8	1.381 (2)	C13—C14	1.390 (3)
N1—C1	1.417 (2)	C13—H13	0.96 (3)
N1—C16	1.452 (2)	C14—C15	1.382 (3)
C1—C2	1.395 (3)	C14—H14	0.97 (3)
C1—C6	1.397 (3)	C15—H15	0.95 (2)
C2—C3	1.386 (3)	C16—C17	1.515 (3)
C2—H2	0.95 (3)	C16—H16A	0.96 (3)
C3—C4	1.382 (3)	C16—H16B	0.92 (3)
C3—H3	0.94 (3)	C18—C19	1.498 (4)
C4—C5	1.384 (3)	C18—H18A	0.99 (3)
C4—H4	0.93 (3)	C18—H18B	1.00 (3)
C5—C6	1.392 (3)	C19—H19A	1.03 (3)
C5—H5	0.96 (3)	C19—H19B	0.94 (4)
C7—C9	1.343 (3)	C19—H19C	1.00 (4)
C7—C8	1.490 (3)

Cl1···C14ⁱ	3.5363 (9)	O1···H12ⁱ	2.560 (9)
Cl1···S1ⁱ	3.7268 (3)	O1···H15ⁱⁱ	2.400 (8)
Cl1···C10ⁱ	3.5940 (7)	O2···H18B	2.545 (11)
Cl1···C15ⁱ	3.4359 (7)	O2···H13ⁱ	2.606 (10)
Cl1···H9	2.674 (8)	O2···H18A	2.74 (3)
S1···N1	3.0100 (6)	O3···H16B^vi	2.666 (8)
S1···C15	3.1748 (8)	C2···C17	3.2932 (10)
S1···O1ⁱⁱ	3.4189 (6)	C4···C14ⁱⁱⁱ	3.5882 (12)
S1···H15	2.660 (10)	C2···H16B	2.621 (8)
S1···H5ⁱⁱⁱ	2.906 (9)	C4···H14ⁱⁱⁱ	2.826 (10)
O1···C17	3.1263 (9)	C4···H12^vii	2.993 (10)
O1···C12ⁱ	3.2141 (10)	C5···H12^vii	2.817 (10)
O1···C15ⁱⁱ	3.2268 (8)	C7···H15	2.937 (9)
O2···N1	2.7902 (7)	C15···H16Aⁱⁱ	2.900 (9)
O2···C8	3.2091 (9)	C16···H16B^vi	2.987 (9)
O2···C1	3.3715 (8)	C16···H2	2.538 (9)
O2···C3^iv	3.3498 (10)	C17···H2	2.696 (9)
O2···C2	3.4103 (9)	H2···H16B	2.223 (12)
O1···H9	2.516 (9)	H5···H12^vii	2.444 (13)
O1···H16A	2.376 (9)	H5···H15ⁱⁱⁱ	2.509 (12)
O1···H4^v	2.806 (11)	H16B···H16B^vi	2.381 (13)

C6—S1—C7	98.19 (9)	C12—C11—Cl1	117.82 (16)
C17—O3—C18	116.60 (16)	C10—C11—Cl1	119.98 (16)
C8—N1—C1	124.52 (15)	C11—C12—C13	119.92 (19)
C8—N1—C16	115.56 (16)	C11—C12—H12	118.0 (16)
C1—N1—C16	118.47 (16)	C13—C12—H12	122.0 (16)
C2—C1—C6	118.60 (18)	C12—C13—C14	119.4 (2)
C2—C1—N1	121.41 (17)	C12—C13—H13	118.8 (16)
C6—C1—N1	120.00 (17)	C14—C13—H13	121.8 (16)
C3—C2—C1	120.63 (19)	C15—C14—C13	120.3 (2)
C3—C2—H2	120.0 (15)	C15—C14—H14	119.2 (16)
C1—C2—H2	119.4 (15)	C13—C14—H14	120.5 (16)
C4—C3—C2	120.6 (2)	C14—C15—C10	121.74 (18)
C4—C3—H3	120.2 (16)	C14—C15—H15	120.3 (15)
C2—C3—H3	119.2 (16)	C10—C15—H15	118.0 (15)
C3—C4—C5	119.4 (2)	N1—C16—C17	112.65 (16)
C3—C4—H4	120.0 (18)	N1—C16—H16A	109.1 (15)
C5—C4—H4	120.6 (18)	C17—C16—H16A	109.0 (14)
C4—C5—C6	120.5 (2)	N1—C16—H16B	109.1 (15)
C4—C5—H5	119.4 (15)	C17—C16—H16B	110.8 (15)
C6—C5—H5	120.1 (15)	H16A—C16—H16B	106 (2)
C5—C6—C1	120.27 (18)	O2—C17—O3	125.18 (19)
C5—C6—S1	119.21 (15)	O2—C17—C16	125.21 (18)
C1—C6—S1	120.51 (15)	O3—C17—C16	109.61 (16)
C9—C7—C8	118.34 (17)	O3—C18—C19	107.0 (2)
C9—C7—S1	125.50 (16)	O3—C18—H18A	106.6 (17)
C8—C7—S1	116.11 (14)	C19—C18—H18A	113.0 (17)
O1—C8—N1	120.36 (17)	O3—C18—H18B	109.1 (17)
O1—C8—C7	121.99 (17)	C19—C18—H18B	112.6 (18)
N1—C8—C7	117.64 (16)	H18A—C18—H18B	108 (2)
C7—C9—C10	127.75 (19)	C18—C19—H19A	111.0 (18)
C7—C9—H9	116.7 (15)	C18—C19—H19B	108 (2)
C10—C9—H9	115.3 (15)	H19A—C19—H19B	109 (3)
C15—C10—C11	116.42 (18)	C18—C19—H19C	112.1 (19)
C15—C10—C9	123.16 (17)	H19A—C19—H19C	107 (2)
C11—C10—C9	120.39 (18)	H19B—C19—H19C	111 (3)
C12—C11—C10	122.19 (19)

C8—N1—C1—C2	149.38 (19)	C9—C7—C8—N1	−152.68 (18)
C16—N1—C1—C2	−16.2 (3)	S1—C7—C8—N1	29.5 (2)
C8—N1—C1—C6	−31.3 (3)	C8—C7—C9—C10	175.30 (18)
C16—N1—C1—C6	163.13 (17)	S1—C7—C9—C10	−7.1 (3)
C6—C1—C2—C3	−0.8 (3)	C7—C9—C10—C15	37.4 (3)
N1—C1—C2—C3	178.55 (18)	C7—C9—C10—C11	−140.4 (2)
C1—C2—C3—C4	0.8 (3)	C15—C10—C11—C12	−2.9 (3)
C2—C3—C4—C5	0.3 (3)	C9—C10—C11—C12	175.1 (2)
C3—C4—C5—C6	−1.3 (3)	C15—C10—C11—Cl1	178.57 (15)
C4—C5—C6—C1	1.2 (3)	C9—C10—C11—Cl1	−3.5 (3)
C4—C5—C6—S1	−178.03 (16)	C10—C11—C12—C13	1.7 (3)
C2—C1—C6—C5	−0.2 (3)	Cl1—C11—C12—C13	−179.76 (18)
N1—C1—C6—C5	−179.53 (17)	C11—C12—C13—C14	0.7 (3)
C2—C1—C6—S1	179.07 (15)	C12—C13—C14—C15	−1.8 (3)
N1—C1—C6—S1	−0.3 (2)	C13—C14—C15—C10	0.5 (3)
C7—S1—C6—C5	−146.23 (16)	C11—C10—C15—C14	1.8 (3)
C7—S1—C6—C1	34.53 (17)	C9—C10—C15—C14	−176.10 (19)
C6—S1—C7—C9	134.12 (18)	C8—N1—C16—C17	−80.3 (2)
C6—S1—C7—C8	−48.28 (16)	C1—N1—C16—C17	86.5 (2)
C1—N1—C8—O1	−165.71 (18)	C18—O3—C17—O2	−0.1 (3)
C16—N1—C8—O1	0.3 (3)	C18—O3—C17—C16	−179.95 (18)
C1—N1—C8—C7	14.8 (3)	N1—C16—C17—O2	1.2 (3)
C16—N1—C8—C7	−179.20 (16)	N1—C16—C17—O3	−179.02 (16)
C9—C7—C8—O1	27.9 (3)	C17—O3—C18—C19	−171.0 (2)
S1—C7—C8—O1	−149.91 (15)

Symmetry codes: (i) −x+1, y−1/2, −z+1/2; (ii) −x+1, −y+1, −z+1; (iii) −x+1, −y+2, −z+1; (iv) −x, y−1/2, −z+1/2; (v) x, y−1, z; (vi) −x, −y+1, −z+1; (vii) −x+1, y+1/2, −z+1/2.

Hydrogen-bond geometry (Å, º) top

D—H···A	D—H	H···A	D···A	D—H···A
C12—H12···O1^vii	0.95 (3)	2.56 (3)	3.214 (2)	126 (2)
C15—H15···O1ⁱⁱ	0.95 (2)	2.40 (2)	3.227 (2)	145.8 (15)

Symmetry codes: (ii) −x+1, −y+1, −z+1; (vii) −x+1, y+1/2, −z+1/2.

Comparison of selected (X-ray and DFT) geometric data (Å, °) top

Bonds/angles	X-ray	B3LYP/6-311G(d,p)
Cl1—C11	1.741 (2)	1.83593
S1—C6	1.755 (2)	1.83362
S1—C7	1.757 (2)	1.79349
O1—C8	1.224 (2)	1.26839
O2—C17	1.200 (2)	1.23993
O3—C17	1.335 (2)	1.36867
O3—C18	1.462 (3)	1.48321
N1—C8	1.381 (2)	1.40044
N1—C1	1.417 (2)	1.41683
N1—C16	1.452 (2)	1.47008

C6—S1—C7	98.19 (9)	99.41730
C17—O3—C18	116.60 (16)	116.97676
C8—N1—C1	124.52 (15)	125.49531
C8—N1—C16	115.56 (16)	115.02066
C1—N1—C16	118.47 (16)	118.38057
C2—C1—N1	121.41 (17)	121.23845
C2—C1—C6	118.60 (18)	117.94010
C6—C1—N1	120.00 (17)	120.81444
O1—C8—N1	120.36 (17)	120.12402
O1—C8—C7	121.99 (17)	120.12402
N1—C8—C7	117.64 (16)	117.79908

Calculated energies top

Molecular Energy (a.u.) (eV)	Compound (I)
Total Energy, TE (eV)	-50964
E_HOMO (eV)	-5.2696
E_LUMO (eV)	-0.9347
Gap, ΔE (eV)	4.3346
Dipole moment, µ (Debye)	5.6841
Ionisation potential, I (eV)	5.2696
Electron affinity, A	0.9347
Electronegativity, χ	3.1019
Hardness, η	2.1673
Electrophilicity index, ω	2.2198
Softness, σ	0.4614
Fraction of electron transferred, ΔN	0.8993

Minimal inhibitory concentration [MIC (µg mL^-1)] top

ATCC-25923 = Staphylococcus aureus, ATTC-25922 = Escherichia coli, ATCC-27853 = Pseudomonas aeruginosa, Chlor = chloramphenicol and Amp = ampicillin.

Product	ATCC-25923	ATTC-25922	ATCC-27853
(I)	21	21	21
Chlor	58	58	58
Amp	12	12	12
DMSO	0	0	0

Funding information

The support of NSF–MRI grant No. 1228232 for the purchase of the diffractometer and Tulane University for support of the Tulane Crystallography Laboratory are gratefully acknowledged. TH is grateful to the Hacettepe University Scientific Research Project Unit (grant No. 013 D04 602 004).

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Volume 76| Part 5| May 2020| Pages 629-636

https://doi.org/10.1107/S2056989020004119

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research communications\(\def\hfill{\hskip 5em}\def\hfil{\hskip 3em}\def\eqno#1{\hfil {#1}}\)

Crystal structure, Hirshfeld surface analysis and inter­action energy, DFT and anti­bacterial activity studies of ethyl 2-[(2Z)-2-(2-chloro­benzyl­­idene)-3-oxo-3,4-di­hydro-2H-1,4-benzo­thia­zin-4-yl]acetate

1. Chemical context

2. Structural commentary

3. Supra­molecular features

4. Hirshfeld surface analysis

5. Inter­action energy calculations

6. DFT calculations

7. Database survey

8. Anti­bacterial activity

9. Synthesis and crystallization

10. Refinement

Supporting information

Funding information

References

research communications

Crystal structure, Hirshfeld surface analysis and interaction energy, DFT and antibacterial activity studies of ethyl 2-[(2Z)-2-(2-chlorobenzylidene)-3-oxo-3,4-dihydro-2H-1,4-benzothiazin-4-yl]acetate

3. Supramolecular features

5. Interaction energy calculations

8. Antibacterial activity