Preliminary crystallographic studies of glucose dehydrogenase from the promiscuous Entner–Doudoroff pathway in the hyperthermophilic archaeon Sulfolobus solfataricus

Theodossis, A.; Milburn, C.C.; Heyer, N.I.; Lamble, H.J.; Hough, D.W.; Danson, M.J.; Taylor, G.L.

doi:10.1107/S174430910403101X

Acta Crystallographica Section F
Acta Crystallographica
Section F STRUCTURAL BIOLOGY COMMUNICATIONS

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Figure 1
(a) Orthorhombic crystals of SsGDH belonging to space group P2₁2₁2, grown from 0.1 M HEPES pH 6, 13%(w/v) PEG 4000, 8%(v/v) propan-2-ol, 20 mM MgCl₂ with 3.5 mg ml⁻¹ protein. (b) Optimized crystals diffracting to 1.8 Å; the largest crystal is approximately 0.3 × 0.1 × 0.1 mm in size. The black bars correspond to 0.1 mm.

STRUCTURAL BIOLOGY
COMMUNICATIONS

ISSN: 2053-230X

Volume 61| Part 1| December 2005| Pages 112-115

doi:10.1107/S174430910403101X

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