 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 2]](en5068fig2mag.jpg)
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Figure 2
Preparation of reduced and S-pyridylethylated agkicetin-C. 3 mg agkicetin-C was dissolved in 0.5 ml 0.5 M Tris–HCl buffer pH 8.0 containing 6 M guanidine hydrochloride and 2 mM EDTA. 7 mg dithiothreitol was added to the protein solution, mixed and then incubated for 3 h at 323 K. After the addition of 4-vinylpyridine with a 3:1 molar ratio of 4-vinylpyridine:dithiothreitol, the mixture was further incubated for another 3 h at 323 K and then dialyzed against distilled water and 0.1% TFA solution. Finally, the mixture was applied onto a Delta-Pak C4 column (3.9 × 150 mm) and sequentially eluted at room temperature with two linear gradients of acetonitrile solution (0–20% and 20–50% containing 0.1% TFA). |
![[Figure 2]](en5068fig2.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
