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Figure 1
Human Pim-1 kinase samples obtained at different stages of purification. These results were obtained from three independent SDS–PAGE experiments. (a) Raw Pim-1 protein sample obtained by digesting the GST-fusion protein using 5000 units of thrombin followed by anion-exchange chromatography. (b) Pim-1 protein obtained after a second digestion with an additional 10 000 units of thrombin. (c) Pim-1 protein purified using anion-exchange chromatography from twice-digested GST-fusion Pim-1 protein. (d) Pure Pim-1 protein obtained using preparative SEC.

ISSN: 2053-230X
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