Figure 2
SDS–PAGE analysis of TdcD during purification. Proteins were analysed on 10% SDS–PAGE and stained with Coomassie blue. Lane 1, crude cell lysates after 0.3 mM IPTG induction; lane 2, clear supernatant; lane 3, purified TdcD after Ni–NTA affinity column chromatography; lane M, molecular-weight markers (kDa). |