Figure 1
X-ray crystal structure of M. loti NAT1. (a) and (b) Orthogonal secondary-structure representation of M. loti NAT1 (mauve) superimposed with worm representations of the NAT structures from Salmonella typhimurium (cyan; PDB code 1e2t
), Mycobacterium smegmatis (yellow; PDB code 1gx3
) and Pseudomonas aeruginosa (green; PDB code 1w4t
) using the program O (Jones et al., 1991). The M. loti NAT1 active-site catalytic triad is also shown in ball-and-stick representation. (c) Surface representation of M. smegmatis NAT1 (yellow) and M. loti NAT1 (mauve) in the region of the active-site entrance. Underlying secondary-structural elements are drawn in a similar colour scheme. For clarity, the α6 helix, corresponding to residues 183–205 in the M. loti structure, is not shown in this figure. The active-site catalytic triad is also shown in ball-and-stick mode to aid orientation. All images were generated using AESOP (M. E. M. Noble, Oxford University, England). |