Figure 1
Self-rotation function and native Patterson. (a) Self-rotation function calculated using CNS (Brünger et al., 1998) in the resolution range 15–4.5 Å, integration radius 5–39 Å. Section at κ = 120° showing the threefold peak (9.4σ) aligned with one of the crystallographic 222 axes. Contours start at the 1σ level and increase in 1σ intervals. (b) Native Patterson map calculated using the CCP4 suite (Collaborative Computational Project, Number 4, 1994) in the resolution range 35–4 Å for a primitive orthorhombic lattice with unit-cell parameters a = 130.1, b = 78.7, c = 171.2 Å. Contours start at 3σ and increase in steps of 2σ. Left, section w = 0 showing the origin peak and that at (0.5, 0.49, 0) thought to arise from the pseudo-hexameric symmetry of P2 trimers. Right, section w = 0.5. A peak is evident at position (0.43, 0.5, 0.5). Prepared using the program GROPAT (R. Esnouf & D. I. Stuart, unpublished program). |