view article
Figure 1
SDS–PAGE showing the two major steps in the purification of HemS from Y. enterocolitica. (a) His-tag purification. Lane 1, molecular-weight markers (kDa); lane 2, flowthrough; lanes 3, 4, 5 and 6, washing with 5, 10, 15 and 20 mM imidazole, respectively; lane 7, elution of bound HemS with 150 mM imidazole. (b) Purified HemS protein samples before (lane 2) and after (lane 3) cleavage with thrombin.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
Volume 61| Part 8| July 2005| Pages 802-805
doi:10.1107/S1744309105023523
Follow Acta Cryst. F
Sign up for e-alerts
E-alerts
Follow Acta Cryst. on Twitter
Twitter
Follow us on facebook
Facebook
Sign up for RSS feeds
RSS