 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 2]](mp5005fig2mag.jpg)
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Figure 2
(a) Hsc70ΔCterm crystals obtained in initial screens (microbatch under oil: 1 µl of 20 mg ml−1 protein in 10 mM Tris pH 8.0, 1 mM EDTA, 1 mM DTT, 50% glycerol plus 1 µl 0.2 M sodium acetate, 0.1 M Tris 8.5, 30% PEG 4000). (b) Optimized crystals [inset; grown as in (a) but with 0.5 M KI added] and diffraction pattern. (c) Further optimization of crystals [inset; grown as in (b) but the protein was further purified by cation-exchange and gel-exclusion chromatography] and a representative diffraction pattern (P212121; a = 78.3, b = 95.2, c = 178.5 Å). |
![[Figure 2]](mp5005fig2.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
