|
Figure 4
Native PAGE of wild-type (WT) and mutant bHsc70ΔCterms. Above each lane the type of residues mutated and the net effect on the protein's charge are given (i.e. in lane 1 DKK to AAA changes one negative and two positive side chains to neutral, leading to a net change of −1). Changes in electrophoretic migration are consistent with the expected net changes in charge. |