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Figure 5
Crystal of Hsc70ΔCtermE213A/D214A mutant (macrobatch under oil at 289 K: 5 µl of 13 mg ml−1 protein in 10 mM Tris pH 8.0, 50% glycerol, 1 mM EDTA, 1 mM DTT plus 5 µl of 4 M TMAO plus 10 µl of 18% PEG 8000). |
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Figure 5
Crystal of Hsc70ΔCtermE213A/D214A mutant (macrobatch under oil at 289 K: 5 µl of 13 mg ml−1 protein in 10 mM Tris pH 8.0, 50% glycerol, 1 mM EDTA, 1 mM DTT plus 5 µl of 4 M TMAO plus 10 µl of 18% PEG 8000). |