crystallization communications
Overexpression, purification, crystallization and data collection of Sulfolobus solfataricus Sso6206, a novel highly conserved protein
aThe Centre for Biomolecular Sciences, The University, St Andrews KY16 9ST, Scotland
*Correspondence e-mail: naismith@st-and.ac.uk
Sso6206, a 10.5 kDa protein from Sulfolobus solfataricus, has been overexpressed, purified and crystallized. The protein crystallizes in P61/522, with unit-cell parameters a = b = 157.8, c = 307.3 Å. The crystals are hexagonal bipyramids and a data set has been collected to 2.4 Å resolution. cannot be attempted as no convincing model can be identified. Crystals of selenomethionine-variant protein have not yet been obtained. Interestingly, crystal packing, gel filtration and all suggest the native protein forms a multi-subunit oligomer consisting of >9 subunits.
Keywords: Sso6206; Sulfolobus solfataricus.
1. Introduction
Sso6206 is a small acidic protein (MW 10 483 Da, pI 4.5) encoded in the genome of the crenarchaeon Sulfolobus solfataricus (She et al., 2001) with an unknown function. It is nearly ubiquitous in the archaea, including crenarchaea and euryarchaea, hyperthermophiles, psychrophiles and In addition, homologues have been annotated in two bacterial species, Shewanella frigidimarina, a marine member of the gamma proteobacteria (Bowman et al., 1997), and Desulfotalea psychrophila, a sulfate-reducing delta proteobacterium from arctic sediments (Knoblauch et al., 1999). The limited distribution in bacteria suggests that these two species have obtained the gene by lateral gene transfer from an archaeon. The amino-acid sequence of the protein is highly conserved (Fig. 1), with greater than 40% identity observed between archaeal family members. Perhaps significantly, there are four conserved acidic residues and a conserved histidine (coloured red and blue, respectively, in Fig. 1), which are often ligands for bound metal ions. The strong conservation hints at a role for this protein in the archaea, making it an interesting target for structural studies. The protein belongs to Pfam2680, a family which has yet to be characterized. Here, we report the overexpression, crystallization and native data collection of recombinant Sso6206.
2. Materials and methods
2.1. Cloning, overexpression and purification
The Sso6206 gene was amplified using the genomic DNA of Sulfolobus solfataricus P2 as a template with a 5′-end oligonucleotide primer (5′-GGCGCCATGGATGGCAATTAGAAGACTTGTTTTAG-3′) containing an NcoI site and a 3′-end primer (5′-GCTGGGATCCCTACAAATCATCCTTTATCTTTCCCTC-3′) containing a BamHI site. The amplified DNA fragment was digested with NcoI/BamHI and then ligated into an NcoI/BamHI linearized pEHISTEV vector (Liu & Naismith, unpublished work) such that six histidine residues and a tobacco etch virus (TEV) protease site were added to the N-terminus of the protein in order to facilitate automated protein purification. After protease cleavage, the resulting protein has an additional glycine and alanine at the N-terminus.
The recombinant plasmid was transformed into Escherichia coli BL21 (DE3) cells (Novagen) and four 500 ml cultures were grown in Luria–Bertani medium (Formedia) supplemented with 30 µg ml−1 kanamycin at 310 K until A600 reached 0.6. The cultured cells were induced with 1 mM isopropyl β-D-thiogalactopyranoside (IPTG) and incubated for 4 h at 298 K. The cells were harvested by centrifugation (5000 rev min−1, 15 min, 277 K), washed with PBS and re-spun. The cells were stored at 193 K until required. For purification, the cell pellet was resuspended in lysis buffer (25 mM Tris–HCl pH 7.75, 0.6 M NaCl, 30 mM imidazole, 20 µM lysozyme and 20 µg ml−1 DNAse I) and lysed by a Constant Systems cell disruptor at 298 K. The crude lysate was centrifuged (15 000 rev min−1, 25 min, 277 K) and the supernatant fraction was passed through a 0.22 µm PES syringe filter (Millipore). The supernatant was passed over a nickel-affinity column and the protein eluted in a single step with elution buffer (25 mM Tris–HCl pH 7.75, 0.6 M NaCl, 250 mM imidazole). After removal of the imidazole, the eluted protein was then incubated with 1 ml of 1 mg ml−1 tobacco etch virus protease (approximate 20:1 ratio protein:protease). The protein was then applied onto an S-200 column and fractions were assayed by SDS–PAGE gel The His-tagged and cleaved protein behave similarly during gel filtration and the presence or absence of 20 mM DTT does not significantly alter the elution profile of either protein. The fractions containing homogeneous Sso6206 were combined. It was estimated that a total of 20 mg pure Sso6206 was obtained from 2 l cell culture. The protein was concentrated to 6 mg ml−1 as measured by Bradford assay. The identity and integrity of the protein were confirmed by (University of St Andrews).
2.2. Crystallization
Initial conditions were obtained using a sitting-drop vapour-diffusion screen of commercial sparse-matrix crystallization conditions. Three protein concentrations (6, 3 and 1 mg ml−1) were screened at 293 K with a drop size of 0.2 µl (containing 0.1 µl protein solution and 0.1 µl precipitant solution) prepared using a nanodrop crystallization robot (Cartesian HoneyBee) as a part of the Hamilton–Thermo Rhombix system. Optimization of the initial 12 most promising hits, including at least one at each concentration, were performed to confirm that the crystals contained protein. The largest protein crystals were obtained using the hanging-drop vapour-diffusion method (2 µl + 2 µl) at 293 K. Improved crystals could be obtained from a number of conditions; however, the best crystals, as judged by size and regular shape, were obtained from variation of Wizard I (Emerald Biosystems) condition Nos. 31 [20%(w/v) PEG 8000, 0.1 M phosphate–citrate pH 4.2, 0.2 M NaCl] and 36 (1.0 M sodium citrate, 0.1 M imidazole pH 8.0). The crystals obtained from both conditions were of the protein and had hexagonal bipyramidal morphology (0.4 × 0.4 × 0.4 mm). The crystals appear as a spherical precipitate within 2 d and gradually develop more distinct edges over the course of a month. showed that the crystals obtained from a precipitant of 0.6 M sodium citrate, 0.1 mM imidazole pH 7.75 with a protein concentration of 5 mg ml−1 were optimal (Fig. 2). We have been able to express the selenomethionine variant of the protein using methioinine inhibition (Doublié, 1997). The protein was purified in the same way as the native protein. confirmed full incorporation of selenium and did not indicate that any oxidation had occurred. The selenomethionine protein appeared to be homogenous by and SDS–PAGE gel However, we have so far been unable to crystallize the selenomethionine variant of the protein either using the native conditions or in a re-screen of sparse-matrix conditions. Circular-dichroism spectrometry suggests the selenomethionine protein is not significantly different and the protein has a similar profile to the native protein on size-exclusion chromatography.
2.3. X-ray data collection
A 2.7 Å data set was collected in-house at 130 K from a single crystal mounted on a loop. The crystal was subject to a soaking in 15%(v/v) ethylene glycol in stabilization buffer before transferring to cryogenic conditions for data collection. Data was collected as 20 min 0.5° oscillations using a Rigaku HTC image-plate detector, a rotating-anode X-ray source and Osmic mirrors. The crystal-to-detector distance was 220 mm. All data were integrated using MOSFLM (Leslie, 1992) and merged with SCALA (Evans, 1997) as implemented in CCP4 (Collaborative Computational Project, Number 4, 1994). The initial indexing showed the crystals to be primitive hexagonal, with unit-cell parameters a = b = 158.1, c = 308.3 Å, α = β = 90, γ = 120°; a second native data set was collected at Daresbury which showed similar and resolution. A third data set was collected at the European Scientific Research Facility (ESRF) Grenoble with the same and unit-cell parameters and improved resolution (Fig. 3). Data-collection statistics are given in Table 1. Merging of the data indicates that the belongs to the higher symmetry 6/mmm Analysis of over 100 00l axial reflections shows a clear l = 6n condition, indicating that the is P6122 or its P6522.
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3. Results and discussion
There is no convincing sequence match between Sso6206 and any structure in the PDB. This rules out λ = 1.488 Å, data not shown). We will continue with heavy-metal soaks and attempt to use sulfur phasing to obtain a structure.
as a phasing strategy. Several attempts to incorporate heavy atoms by soaking into existing crystals or by cocrystallization with recombinant protein have proven unsuccessful in that the crystals which grew or survived soaking did not show a clear anomalous diffraction when tested at the SRS Daresbury (The unit-cell parameters are consistent with an VM = 3.50 Å3 Da−1, solvent content 65%) and 30 (VM = 1.77 Å3 Da−1, solvent content 30%) monomers. The relatively low diffraction resolution of the crystals suggests a higher solvent content. Interestingly, the protein elutes very far from the expected elution point for a 10 kDa protein on a Sephacryl S-200 column. On this column globular proteins in excess of 200 kDa elute in the void volume and Sso6206 does not elute in the void volume. Molecular-weight calibration of the column suggest Sso6206 is over 100 kDa; more accurate analysis is not reliable. This suggests that Sso6206 contains in excess of ten monomers per protomer (100 kDa). As well as a peak corresponding to monomeric protein, the contains peaks that correspond to multimers (up to hexamers can be distinguished). Together, these data suggest that Sso6206 forms a large multimeric protomer. If such an oligomer is indeed found for the protein, it is likely to have important functional consequences. Intriguingly, neither the self-rotation function nor the native Patterson show any additional peaks which would suggest rotational or translational noncrystallographic symmetry.
content of between 15 (Acknowledgements
The protein was targeted as part of the Scottish Structural Proteomics Facility (SSPF), which is funded by the Scottish Higher Education Funding Council (SHEFC) and the Biotechnology and Biological Sciences Research Council UK (BBSRC). JHN is a BBSRC Career Development Fellow.
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