crystallization communications
Purification, crystallization and preliminary X-ray diffraction studies of N-acetylglucosamine-phosphate mutase from Candida albicans
aDepartment of Chemistry, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan, bKamakura Research Laboratory, Chugai Pharmaceutical Co. Ltd, 200 Kajiwara, Kamakura, Kanagawa 247-8530, Japan, cDepartment of Hygiene, School of Medicine, Yokohama City University, 3-9 Fukuura, Kanazawa, Yokohama 236-0004, Japan, and dRIKEN SPring-8 Center at Harima Institute, Koto 1-1-1, Mikazukicho, Sayo-gun, Hyogo 679-5148, Japan
*Correspondence e-mail: miki@kuchem.kyoto-u.ac.jp
N-acetylglucosamine-phosphate mutase (AGM1) is an essential enzyme in the synthesis of UDP-N-acetylglucosamine (UDP-GlcNAc) in eukaryotes and belongs to the α-D-phosphohexomutase superfamily. AGM1 from Candida albicans (CaAGM1) was purified and crystallized by the sitting-drop vapour-diffusion method. The crystals obtained belong to the primitive monoclinic P21, with unit-cell parameters a = 60.2, b = 130.2, c = 78.0 Å, β = 106.7°. The crystals diffract X-rays to beyond 1.8 Å resolution using synchrotron radiation.
Keywords: N-acetylglucosamine-phosphate mutase; Candida albicans.
1. Introduction
UDP-N-acetylglucosamine (UDP-GlcNAc) is a UDP sugar and is synthesized from fructose-6-phosphate supplied by the glycolytic pathway (Watzele & Tanner, 1989; Mio et al., 1999; Hofmann et al., 1994; Mio et al., 1998). UDP-GlcNAc is used as a precusor in the synthesis of cell-wall chitin and Although UDP-GlcNAc is also utilized in mammalian cells, fungi require a larger amount of UDP-GlcNAc owing to the synthesis of the cell wall.
N-Acetylglucosamine-phosphate mutase (AGM1; EC 5.4.2.3) is an essential enzyme in the synthesis of UDP-GlcNAc. AGM1 catalyzes the intramolecular transfer of a phosphoryl group. This synthetic step consists of the interconversion of N-acetylglucosamine-6-phosphate (GlcNAc-6-P) and N-acetylglucosamine-1-phosphate (GlcNAc-1-P). The process of UDP-GlcNAc biosynthesis differs in prokaryotes and eukaryotes and AGM1 has only been identified in eukaryotes (Mio et al., 2000). It is well known that AGM1 requires Mg2+ ions to exhibit maximum activity; complete inhibition has also been achieved with Zn2+ ions (Cheng & Carlson, 1979; Fernandez-Sorensen & Carlson, 1971). AGM1 is a member of the α-D-phosphohexomutase superfamily, as it catalyzes a reversible intramolecular phosphoryl transfer on its sugar substrate (Shackelford et al., 2004; Whitehouse et al., 1998). Other superfamily members exist as various oligomeric states (monomers, dimers and trimers) in solution (Jolly et al., 1999; Maino & Young, 1974). Phosphomannomutase/phosphoglucomutase from Pseudomonas aeruginosa (PaPMM/PGM; PDB code 1k2y), which catalyzes the reversible conversion of mannose-6-phosphate to mannose-1-phosphate and glucose-6-phosphate to glucose-1-phosphate, shares 20.7% sequence identity with AGM1 from Candida albicans (CaAGM1) as calculated by the program FASTA (Lipman & Pearson, 1985; Pearson & Lipman, 1988) and exists as a monomer in solution (Regni et al., 2002, 2004). Several structures of enzymes in this superfamily have been reported to date. They are similar to each other, being composed of four domains arranged in a `heart shape' (Liu, 1997; Regni et al., 2002, 2004). However, for AGM1 only the atomic coordinates of the C-terminal domain from Mus musculus (112 amino-acid residues of 542 amino-acid residues) has been deposited (PDB code 1wjw) and overall structures have not been determined. Multiple sequence alignment in the superfamily shows that AGM1 has significant sequence similarity to other members with regard to the presumed active-site loops, but that AGM1 has a different relative location of several loops; these loops were not identified as affecting the activity by previous mutation analysis (Shackelford et al., 2004; Mio et al., 2000). Also, little is known about the substrate specificity. Determination of the three-dimensional structure of this enzyme is thus necessary in order to gain a better understanding not only of its catalytic mechanism, but also of the additional important differences betweeen the enzymes included in this superfamily.
Here, we describe the purification, crystallization and preliminary X-ray data for CaAGM1. C. albicans is a disease-causing fungus that leads to a life-threatening systemic infection (referred to as `candidasis') in immunocompromized hosts (Rogers & Balish, 1980). It is impossible for fungi to live without biosynthesis of the cell wall and the chitin of the cell wall is synthesized from UDP-GlcNAc. A specific inhibitor of AGM1 from pathogenic fungi could therefore be a new candidate antifungal reagent that would inhibit cell-wall synthesis.
2. Purification
CaAGM1 is composed of 544 amino-acid residues and its molecular weight is about 60 kDa. For the crystallization experiments, the ORF of CaAGM1 was cloned between the BamHI and EcoRI sites of pGEX-2T to express CaAGM1 as a glutathione S-transferase (GST) fusion. CaAGM1 was overproduced and sonicated by previously described methods (Mio et al., 2000), except that after the addition of isopropyl 1-thio-galactopyranoside the cells were grown for an additional 22 h at 295 K. The soluble fraction was applied onto a Glutathione Sepharose 4 FastFlow column (Amersham Biosciences, Piscataway, NJ, USA) and was washed with PBS at 277 K. The purified protein was eluted with a GST elution buffer (20 mM Tris–HCl, 0.15 M NaCl, 10 mM glutathione pH 8.5). The GST was then removed by a 16 h treatment with thrombin at 277 K. Finally, this solution was applied onto a DEAE-Toyopearl 650 column (Tosoh, Tokyo, Japan) and eluted using a gradient of 0–0.5 M NaCl contained in elution buffer [20 mM Tris–HCl, 1 mM EDTA, 5%(v/v) glycerol pH 7.5] at 277 K. The purity of the fraction used for crystallization was tested by SDS–PAGE. Gel-filtration analysis (data not shown) suggested that the purified protein exists as a monomer in solution. The final yield of CaAGM1 per litre of culture is approximately 2.5 mg. It was desalted and concentrated to a volume of 10 mg ml−1.
3. Crystallization
Crystallization attempts were carried out using Crystal Screens 1 and 2, Crystal Screen Cryo and PEG/Ion Screen (Hampton Research, Aliso Viejo, CA, USA). All attempts at crystallization were performed using the sitting-drop vapour-diffusion method at 293 K. Mixtures of 1.25 µl protein solution (10 mg ml−1 in 50 mM Tris–HCl pH 7.6 and 1 mM dithiothreitol) and an equal volume of the reservoir solution were equilibrated against 100 µl reservoir solution. Crystals were obtained using reservoir solution containing 200 mM NH4H2PO4 and 14–20%(w/v) polyethylene glycol 3350 within a week with approximate dimensions of 0.5 × 0.2 × 0.03 mm (Fig. 1).
4. X-ray data collection
The crystals were soaked briefly in a cryoprotectant solution prepared by the addition of 15%(v/v) glycerol to the reservoir solution and the crystals were mounted in a loop and flash-cooled in an N2 gas stream at 95 K. X-ray diffraction studies were performed at SPring-8 (BL44B2) and the Photon Factory (BL5). The diffraction data used for the were collected at BL44B2 with a MAR CCD detector using an oscillation angle of 0.5°, 15 s exposure per frame and a wavelength of 1.000 Å. The crystal-to-detector distance was set at 115 mm. Diffraction was observed to a resolution exceeding 1.8 Å. The data set from a single crystal was processed and scaled using the programs DENZO and SCALEPACK from the HKL2000 package (Otwinowski & Minor, 1997).
The crystals were found to belong to the primitive monoclinic system and k = 2n + 1 at 0k0) indicated the of the data set to be P21. The statistics for the intensity data are shown in Table 1. Assuming the presence of two CaAGM1 molecules per the calculated Matthews coefficient VM was 2.42 Å3 Da−1 (Matthews, 1968), which corresponds to a solvent content of 49.2%. Phasing by the multiple method and the structure were successful and the structure will be published soon.
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Acknowledgements
We would like to thank Dr Satoshi Sogabe for help with the biochemical experiments. We are also grateful to the beamline scientists at SPring-8 and the Photon Factory. This work was supported by a grant from the National Project on Protein Structural and Functional Analyses from the Ministry of Education, Culture, Sports, Science and Technology of Japan.
References
Cheng, P. W. & Carlson, D. M. (1979). J. Biol. Chem. 254, 8353–8357. CAS PubMed Web of Science Google Scholar
Fernandez-Sorensen, A. & Carlson, D. M. (1971). J. Biol. Chem. 246, 3485–3493. CAS PubMed Web of Science Google Scholar
Hofmann, M., Boles, E. & Zimmermann, F. K. (1994). Eur. J. Biochem. 221, 741–747. CrossRef CAS PubMed Web of Science Google Scholar
Jolly, L., Ferrari, P., Blanot, D., Van Heijenoort, J., Fassy, F. & Mengin-Lecreulx, D. (1999). Eur. J. Biochem. 262, 202–210. Web of Science CrossRef PubMed CAS Google Scholar
Lipman, D. J. & Pearson, W. R. (1985). Science, 227, 1435–1441. CrossRef CAS PubMed Web of Science Google Scholar
Liu, Y. (1997). Acta Cryst. D53, 392–405. CrossRef CAS Web of Science IUCr Journals Google Scholar
Maino, V. C. & Young, F. E. (1974). J. Biol. Chem. 249, 5176–5181. CAS PubMed Web of Science Google Scholar
Matthews, B. W. (1968). J. Mol. Biol. 33, 491–497. CrossRef CAS PubMed Web of Science Google Scholar
Mio, T., Yabe, T., Arisawa, M. & Yamada-Okabe, H. (1998). J. Biol. Chem. 273, 14392–14397. Web of Science CrossRef CAS PubMed Google Scholar
Mio, T., Yamada-Okabe, T., Arisawa, M. & Yamada-Okabe, H. (1999). J. Biol. Chem. 274, 424–429. Web of Science CrossRef CAS PubMed Google Scholar
Mio, T., Yamada-Okabe, T., Arisawa, M. & Yamada-Okabe, H. (2000). Biochim. Biophys. Acta, 1492, 369–376. Web of Science CrossRef PubMed CAS Google Scholar
Otwinowski, Z. & Minor, W. (1997). Methods Enzymol. 276, 307–326. CrossRef CAS PubMed Web of Science Google Scholar
Pearson, W. R. & Lipman, D. J. (1988). Proc. Natl Acad. Sci. USA, 85, 2444–2448. CrossRef CAS PubMed Web of Science Google Scholar
Regni, C., Naught, L., Tipton, P. A. & Beamer, L. J. (2004). Structure, 12, 55–63. Web of Science CrossRef PubMed CAS Google Scholar
Regni, C., Tipton, P. A. & Beamer, L. J. (2002). Structure, 10, 269–279. Web of Science CrossRef PubMed CAS Google Scholar
Rogers, T. J. & Balish, E. (1980). Microbiol. Rev. 44, 660–682. CAS PubMed Web of Science Google Scholar
Shackelford, G. S., Regni, C. A. & Beamer, L. J. (2004). Protein Sci. 13, 2130–2138. Web of Science CrossRef PubMed CAS Google Scholar
Watzele, G. & Tanner, W. (1989). J. Biol. Chem. 264, 8753–8758. CAS PubMed Web of Science Google Scholar
Whitehouse, D. B., Tomkins, J., Lovegrove, J. U., Hopkinson, D. A. & McMillan, W. O. (1998). Mol. Biol. Evol. 15, 456–462. Web of Science CrossRef CAS PubMed Google Scholar
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