structural genomics communications
Structure of SAICAR synthase from Thermotoga maritima at 2.2 Å reveals an unusual covalent dimer
aBiosciences Division, Midwest Center for Structural Genomics, Structural Biology Center, Argonne National Laboratory, Argonne, IL 60439, USA, bOntario Centre for Structural Proteomics, University of Toronto, University Health Network, Toronto, Ontario M5G 1L7, Canada, cBanting and Best Department of Medical Research, University of Toronto, Toronto, Ontario M5G 1L6, Canada, and dDepartment of Crystallography, Birkbeck College, Malet Street, London WC1E 7HX, England
*Correspondence e-mail: andrzejj@anl.gov
As a part of a structural genomics program, the 2.2 Å resolution Thermotoga maritima has been solved. This 26.2 kDa protein belongs to the phophoribosylaminoimidazole-succinocarboxamide or SAICAR synthase family of enzymes, the members of which are involved in de novo purine biosynthesis. SAICAR synthase can be divided into three subdomains: two α+β regions exhibiting structural homology with ATP-binding proteins and a carboxy-terminal subdomain of two α-helices. The contains two copies of the protein which are covalently linked by a disulfide bond between Cys126(A) and Cys126(B). This 230-amino-acid protein exhibits high structural homology with SAICAR synthase from baker's yeast. The protein structure is described and compared with that of the ATP–SAICAR synthase complex from yeast.
of the PurC gene product fromKeywords: SAICAR synthase; purine biosynthesis; Thermotoga maritima.
PDB reference: SAICAR synthase, 1kut
1. Introduction
de novo synthesis of purine and pyrimidine is largely invariant among known organisms in biology (Mathews & Van Holde, 1996). Purine biosynthesis proceeds from 5-phopho-α-D-ribosyl-1-pyrophosphate (PRPP) to inosinic acid (IMP) in a multistep pathway. Phosphoribosylaminoimidazole-succinocarboxamide (SAICAR) synthase catalyzes the ATP-dependent transfer of an aspartate molecule to the carboxyl group of 4-carboxy-5-aminoimidazole ribonucleotide. In most prokaryotes, plants and yeast, SAICAR synthase is a monofunctional enzyme. In animals, it is the amino-terminal domain of a larger protein that also exhibits phosphoribosylaminoimidazole carboxylase (AIRC) activity.
are central players in the metabolism of the cell. Not only do they play critical roles in energy metabolism and DNA replication, but they also serve as regulators, signal molecules and enzyme cofactors. As such, the enzymes involved in their biosynthesis may serve as important action points for antimicrobial and anticancer drugs. ThePreviously, the structure of SAICAR synthase from yeast has been determined to 1.9 Å resolution (Levdikov et al., 1998). It is a 34.5 kDa monomer of 306 amino acids folded into three subdomains: two α+β domains and an α-helical carboxy-terminus. A deep central cleft dominates the surface topology, to which all three subdomains contribute residues. By locating highly conserved amino acids and solving the structure of an ATP–SAICAR synthase complex, Ledvikov and coworkers identified the active site as being within this cleft. Most interactions between the adenine base of ATP and the protein involve main-chain atoms, with the exception of Glu219.
As a part of our structural genomics program, we have solved the 2.2 Å resolution Thermotoga maritima. This 26.2 kDa protein is considerably smaller than its relative from yeast (34.5 kDa), with which it shares 26% primary sequence identity and an E value of 0.02 (FASTA/SAS). Nonetheless, the proteins share a common topology and fold. However, in the T. maritima enzyme the crystallographic uniquely contains two copies of SAICAR synthase that are covalently linked by a disulfide bond between Cys126(A) and Cys126(B). The ATP-binding site can be located based upon high structural similarity to the yeast enzyme and other members of the ATP-binding superfamily.
of another member of the SAICAR synthase family, that from2. Materials and methods
2.1. Protein cloning, expression and purification
The TM1243 gene was subcloned, expressed and its product purified and screened for crystallization as described previously (Zhang et al., 2001).
2.2. Protein crystallization
Crystals for X-ray diffraction data collection were obtained from hanging-drop vapor-diffusion conditions containing 2 µl of the SeMet derivative of the protein plus 2 µl 30% PEG 4000, 0.15 M MgCl2, 0.1 M Tris pH 8.5 over 2–5 d at 294 K. The crystals were flash-frozen with crystallization buffer complemented with 25% ethylene glycol. Diffraction intensity data (Table 1) were collected using the Advanced Photon Source (APS) beamline 19ID at Argonne National Laboratory.
‡Figure of merit from §Phasing power = FH/Er.m.s., where FH is the heavy-atom and Er.m.s. is the residual lack of closure. |
2.3. Determination of SAICAR synthase oligomeric state using size-exclusion chromatography
FPLC M HEPES pH 7.5, 0.5 M NaCl. The column was calibrated with cytochrome c (12.4 kDa), carbonic anhydrase (29 kDa), bovine serum albumin (66 kDa), alcohol dehydrogenase (150 kDa), β-amylase (200 kDa) and blue dextran (2000 kDa). A 25 µl SAICAR synthase protein sample at 2 mg ml−1 concentration or premixed with standard proteins was centrifuged at 14 000 rev min−1 for 10 min before being injected into the column through a 20 µl injection loop. Filtration was carried out at 277 K at a flow rate of 1 ml min−1. The eluted proteins were detected by measuring the absorbance at 280 nm. SAICAR synthase elutes as a protein of approximately 53 kDa. This is nearly twice its calculated molecular weight and indicates a dimeric state in solution.
was performed on a Superdex-200 column (10/300 mm) pre-equilibrated with 10 m2.4. Data collection
Diffraction data were collected at 100 K at beamline 19ID of the Structural Biology Center at the Advanced Photon Source, Argonne National Laboratory. Three-wavelength inverse-beam MAD data (peak, 12.6610 keV, 0.9793 Å; inflection point, 12.6640 keV, 0.9791 Å; high-energy remote, 13.0240 keV, 0.9520 Å) were collected from an SeMet-labeled protein crystal to a Bragg spacing of 2.2 Å. One crystal (0.5 × 0.2 × 0.6 mm) was used for data collection at 100 K using 3 s exposures and 1° oscillations at a 200 mm crystal-to-detector distance. The total oscillation range was 160° as predicted using the strategy module within the HKL2000 package (Otwinowski & Minor, 1997). The crystal belonged to P21, with unit-cell parameters a = 63.51, b = 43.07, c = 80.22 Å, β = 92.30°. All data were processed and scaled within HKL2000 to Rmerge values of 8.6, 9.2 and 8.4% for the inflection point, peak and remote data, respectively (Table 1).
2.5. and refinement
The structure was determined by Crystallography & NMR System (CNS; Brünger et al., 1998). The initial model was built manually using QUANTA (Oldfield, 1996). The dimer was refined to an R factor of 24.5% and an Rfree of 28.1% using peak data from 10 to 2.2 Å Bragg spacing. The final model contains 3502 non-H protein atoms and 81 water molecules, with an average overall B value of 30 Å2. Further details can be found in Table 2. Both monomers have a missing surface loop and unmodeled terminal residues, as they were unobserved in the electron-density maps. These include the N-terminus (A1–A6) and A25–A35 of the first monomer and B1, B27–B31 and B199–B201 of the second. Chain A has 213 modelled residues and chain B has 222. The main-chain torsion angles for 89.7% of the residues fall within the most favoured regions of a Ramachandran plot, 8.7% fall in additional allowed areas and the remaining five residues fall in generously allowed areas. No unusual geometries were detected by PROCHECK (Laskowski, 2001).
and refined to 2.2 Å against the averaged peak data using the
|
3. Results and discussion
3.1. Structure description
SAICAR synthase from T. maritima is a 26.2 kDa globular protein composed of segregated α-helical and antiparallel β-sheet regions with approximate dimensions 55 × 45 × 35 Å (Fig. 1). The secondary-structural elements are labeled and numbered as they appear along the polypeptide in Fig. 2, which is a structure-based primary sequence alignment with SAICAR synthase from baker's yeast (Gouet et al., 1999). The monomer can be divided into the same three subdomains as the yeast enzyme: two α+β regions exhibiting structural homology with ATP-grasp fold proteins and the protein kinase superfamily and a final carboxy-terminal subdomain of two α-helices. Each subdomain can be identified in relation to the three longest α-helices in the molecule: α1 (19 residues), α4 (24 residues) and α6 (∼15 residues). The two α+β subdomains each contains a major helix (α1 or α4) and an antiparallel four- to five-stranded β-sheet. As they occur in the molecule, the planes defined by the two β-sheets are roughly perpendicular to each other. A deep central cleft occurs between the two subdomains, with both contributing amino-acid residues to the ATP-binding site. The final subdomain is an α-helical `L' at the C-terminus, with α6 stacking alongside α1 to complete the protein.
3.2. T. maritima SAICAR synthase is a covalent dimer
Two copies of T. maritima SAICAR synthase reside in the crystallographic Pictured in Fig. 3, they form an elongated dimer of roughly 85 × 40 × 30 Å. The amino- and carboxy-termini occur at the far extremes of the dimer and on opposite sides to each other. The central α+β subdomain forms the dimer core and helix α4 lies obliquely across the C-terminal side of the dimer. Short helix α3 straddles the noncrystallographic twofold symmetry axis, with Cys126(A) forming a disulfide bond with Cys126(B) which spans the rotation axis.
One monomer possesses a solvent-accessible surface area of approximately 11 913 Å2. Upon dimer formation, a total of 758 Å2 is lost, as calculated by The Quaternary Structure File Server (PQS) and a solvation free energy of −108 kJ mol−1 is gained (Henrick & Thornton, 1998). 8–9 Å to either side of the disulfide bridge, salt bridges occur between Asp128(A) and Lys145(B) and between Asp128(B) and Lys145(A). The dimer interface is a mixture of hydrophobic and hydrophilic interactions, with a few bound water molecules directly or indirectly hydrogen bonding to each monomer. We observe that a dimer occurs both in solution at 277 K and in the crystal grown at room temperature.
3.3. Comparison to yeast SAICAR synthase and ATP-binding sites
A DALI search for structurally similar proteins (Holm & Sander, 1996) produced one major homolog, with a Z score of 18.3 and a primary sequence identity of 25%: SAICAR synthase from Saccharomyces cerevisiae (PDB code 1a48). Approximately 188 residues of 306 superimpose with the 209-amino-acid T. maritima protein, with an r.m.s.d. of 2.5 Å. Fig. 4 shows a superposition of the T. maritima protein and an ATP-bound complex of the yeast enzyme (PDB code 1odb). The folds are quite similar, with extensive insertions in the yeast protein at the amino- and carboxy-termini as well as in a few loop regions (see Fig. 2 for details). The ATP from the complex locates the binding clefts with conserved glutamates (Glu172 for T. maritima, Glu219 for the yeast enzyme) in equivalent positions. Cys126, responsible for the covalent dimer, and the residues of the interface salt bridge are not conserved in the yeast protein. Furthermore, there is a 13-residue insertion between secondary-structure elements α2 and β7 (see Figs. 2 and 4), which appears to hinder any equivalent dimer formation.
Acknowledgements
We wish to thank all members of the Structural Biology Center at Argonne National Laboratory for their help in conducting experiments and members of the Midwest Center for Structural Genomics for help in preparation of this manuscript. This work was supported by National Institutes of Health Grant GM62414-01 and by the US Department of Energy, Office of Biological and Environmental Research under contract W-31-109-Eng-38
References
Brünger, A. T., Adams, P. D., Clore, G. M., DeLano, W. L., Gros, P., Grosse-Kunstleve, R. W., Jiang, J.-S., Kuszewski, J., Nilges, M., Pannu, N. S., Read, R. J., Rice, L. M., Simonson, T. & Warren, G. L. (1998). Acta Cryst. D54, 905–921. Web of Science CrossRef IUCr Journals Google Scholar
DeLano, W. L. (2002). The PyMOL Molecular Graphics System. https://www.pymol.org. Google Scholar
Gouet, P., Courcelle, E., Stuart, D. I. & Metoz, F. (1999). Bioinformatics, 15, 305–308. Web of Science CrossRef PubMed CAS Google Scholar
Henrick, K. & Thornton, J. M. (1998). Trends Biochem. Sci. 23, 358–361. Web of Science CrossRef CAS PubMed Google Scholar
Holm, L. & Sander, C. (1996). Science, 273, 595–602. CrossRef CAS PubMed Web of Science Google Scholar
Krissinel, E. & Henrick, K. (2004). Acta Cryst. D60, 2256–2268. Web of Science CrossRef CAS IUCr Journals Google Scholar
Laskowski, R. A. (2001). Nucleic Acids Res. 29, 221–222. Web of Science CrossRef PubMed CAS Google Scholar
Levdikov, V. M., Barynin, V. V., Grebenko, A. I., Melik-Adamyan, W. R., Lamzin, V. S. & Wilson, K. S. (1998). Structure, 6, 363–376. Web of Science CrossRef CAS PubMed Google Scholar
Mathews, C. K. & Van Holde, K. E. (1996). Biochemistry. Menlo Park, CA, USA: Benjamin/Cummings. Google Scholar
Oldfield, T. J. (1996). In Crystallographic Computing 7: Proceedings of the Macromolecular Crystallography Computing School, edited by P. Bourne & K. Watenpaugh. Oxford University Press. Google Scholar
Otwinowski, Z. & Minor, W. (1997). Methods Enzymol. 276, 307–326. CrossRef CAS PubMed Web of Science Google Scholar
Zhang, R. G., Skarina, T., Katz, J. E., Beasley, S., Khachatryan, A., Vyas, S., Arrowsmith, C. H., Clarke, S., Edwards, A., Joachimiak, A. & Savchenko, A. (2001). Structure, 9, 1095–106. Web of Science CrossRef PubMed CAS Google Scholar
© International Union of Crystallography. Prior permission is not required to reproduce short quotations, tables and figures from this article, provided the original authors and source are cited. For more information, click here.