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Figure 2
NuPAGE 4–12% Bis-Tris gel (Invitrogen) run with NuPAGE MES buffer. Lane 1, SeeBlue PreStained standard with molecular weights (kDa) given on the left; lane 2, soluble cell fraction; lane 3, Ni–NTA flowthrough; lane 4, Ni–NTA wash; lane 5, Ni–NTA peak elution/Q-Sepharose load; lane 6, Q-Sepharose elution.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
Volume 62| Part 7| June 2006| Pages 672-675
doi:10.1107/S1744309106020616
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