Expression, limited proteolysis and preliminary crystallographic analysis of IpaD, a component of the Shigella flexneri type III secretion system

Johnson, S.; Roversi, P.; Espina, M.; Deane, J.E.; Birket, S.; Picking, W.D.; Blocker, A.; Picking, W.L.; Lea, S.M.

doi:10.1107/S1744309106027047

Acta Crystallographica Section F
Acta Crystallographica
Section F STRUCTURAL BIOLOGY COMMUNICATIONS

IUCr IT WDC

home archive editors for authors for readers submit subscribe open access

view article

Figure 1
(a) Monoclinic crystals of IpaD crystal form 2 produced by in-drop proteolysis. (b) SDS–PAGE of protein derived from crystals with (CF-3 and CF-5) and without (CF-1) subtilisin treatment (see text for full description). The subscript denotes the portion of IpaD present in each band as determined by sequencing (FL, full length; Δ120, N-terminal deletion to residue 120). Molecular-weight markers are shown in lane 2; the marker identified with `M' corresponds to a MW of 42 kDa.

STRUCTURAL BIOLOGY
COMMUNICATIONS

ISSN: 2053-230X

Volume 62| Part 9| August 2006| Pages 865-868

doi:10.1107/S1744309106027047

Follow Acta Cryst. F

Search term		doi		Advanced search
Author		volume	page