Preliminary X-ray diffraction analysis of YcdB from Escherichia coli: a novel haem-containing and Tat-­secreted periplasmic protein with a potential role in iron transport

Cartron, M.L.; Mitchell, S.A.; Woodhall, M.R.; Andrews, S.C.; Watson, K.A.

doi:10.1107/S174430910604509X

Acta Crystallographica Section F
Acta Crystallographica
Section F STRUCTURAL BIOLOGY COMMUNICATIONS

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Figure 2
Crystallization of apo-(His)₆YcdB from E. coli. (a) Feather-like needles observed from initial screening, Macrosol I No. 22; (b) bipyramidal crystals observed from initial screening, Macrosol II No. 14; (c) optimized conditions from (b), 2 M ammonium sulfate, 5% PEG 400, 0.1 M HEPES pH 7.0; (d) solid haem soaked into preformed apo-(His)₆YcdB optimized crystals. The drop size was 2 µl plus 2 µl and the protein concentration was 10 mg ml⁻¹ in all cases.

STRUCTURAL BIOLOGY
COMMUNICATIONS

ISSN: 2053-230X

Volume 63| Part 1| December 2007| Pages 37-41

doi:10.1107/S174430910604509X

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