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Cloning, expression, purification, crystallization and preliminary X-ray of DapA (Rv2753c) from Mycobacterium tuberculosis. Corrigendum
aEMBL Hamburg Outstation, c/o DESY, Notkestrasse 85, D-22603 Hamburg, Germany
*Correspondence e-mail: msweiss@embl-hamburg.de
A correction is made to the Experimental methods section of the article by Kefala & Weiss [(2006), Acta Cryst. F62, 1116–1119].
The first sentence of the Experimental methods section of the article by Kefala & Weiss (2006![[Kefala, G. & Weiss, M. S. (2006). Acta Cryst. F62, 1116-1119.]](../../../../../../logos/arrows/f_arr.gif "Kefala, G. & Weiss, M. S. (2006). Acta Cryst. F62, 1116-1119.")
) should read as follows: Each gram of cell pellet was dissolved in 10 ml buffer A [20 mM Tris pH 8.0, 250 mM NaCl, 10 mM imidazole, 5%(v/v) glycerol, 2 mM β-mercaptoethanol] plus 10%(v/v) 10× BugBuster (Novagen) and one Complete Mini EDTA-free protease-inhibitor cocktail tablet (Roche) per 20 ml, and then lysed by sonication four times for 5 min at a time using 0.25 s pulses at 277 K.
Footnotes
‡Present address: Salk Institute, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
Acknowledgements
The authors would like to thank Dr Simone Weyand, Imperial College London, UK, for pointing out an ommission in the original article.
References
Kefala, G. & Weiss, M. S. (2006). Acta Cryst. F62, 1116–1119. Web of Science CrossRef IUCr Journals Google Scholar
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