Figure 2
Crystal structure of CNanA. Orthogonal views of the CNanA dimer are shown in (a) and (b), where (b) is related to (a) by a 90° rotation about a horizontal axis. The N- and C-termini are indicated by blue and red spheres, respectively. Molecule A (green) and molecule B (cyan) are drawn with the inserted domains (residues 436–535) drawn in lighter shades. The inhibitor Neu5Ac2en is shown in each monomer and is drawn in space-filling mode. (c) Stereoview of the active site of monomer A showing the hydrogen-bond interactions made between Neu5Ac2en and CNanA, with only key amino acids drawn for clarity. The 2Fo − Fc electron-density map contoured at 1σ is only drawn around the inhibitor for clarity. These figures were drawn using PyMOL (DeLano, 2007). |