view article

Figure 2
Schematic catalytic cycle of CuAOs identifying the key intermediates in the reaction pathway. Shown are the type-2 copper centre, the protein-derived cofactor TPQ and the off-metal O2-binding site located in the vicinity of a conserved active-site methionine (Met699 in ECAO). I is the resting state of the enzyme, intermediate II is the product Schiff base, intermediate III is the reduced TPQ or aminoquinol form, intermediate IV shows the proposed off-metal-bound dioxygen species prior to oxygen activation and reduction and intermediate V is the iminoquinone species which hydrolyses to release ammonia, completing the catalytic cycle.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
Follow Acta Cryst. F
Sign up for e-alerts
Follow Acta Cryst. on Twitter
Follow us on facebook
Sign up for RSS feeds