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Figure 1
(a) SDS–PAGE analysis of purified BsLcar. Lane 1, standard molecular-weight markers: phosphorylase b (94 kDa), bovine serum albumin (67 kDa), ovalbumin (45 kDa), carbonic anhydrase (30 kDa), soybean trypsin inhibitor (20.1 kDa) and α-lactoalbumin (14.4 kDa). Lane 2, purified L-N-carbamoylase (∼8 µg). (b) Crystals of recombinant L-N-carbamoylase from G. stearothermophilus CECT43. |