 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 4]](sw5028fig4mag.jpg)
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Figure 4
EMSA of NMB1585–DNA complexes. Increasing amounts of purified NMB1585 protein (20–640 nM) were incubated with a 378 bp DNA probe (20 nM) PCR-amplified from the promoter region of the NMB1585 gene in either the absence (lanes 2–8) or presence (lanes 9–14) of 10 mM sodium salicylate. The DNA–protein complexes were analysed by gel electrophoresis on a tris–borate–EDTA polyacrylamide gel and stained for DNA using SYBR green. Samples are as follows: DNA only (lanes 1 and 8), 20 nM protein (lanes 2 and 9), 40 nM protein (lanes 3 and 10), 80 nM protein (lanes 4 and 11), 160 nM protein (lanes 5 and 12), 320 nM protein (lanes 6 and 13) and 640 nM protein (lanes 7 and 14). |
![[Figure 4]](sw5028fig4.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
