Figure 2
12% Tris–tricine polyacrylamide gel of purified C.Csp231I and dynamic light scattering of sample. (a) Lane M contains Benchmark protein ladder with selected molecular masses highlighted (kDa). Lane FT is the SP-column flowthrough. Lanes 1, 2 and 3 are selected consecutive fractions. (b) Single monodisperse peak corresponding to an Rh value of 2.44 nm (13.5% polydispersity). |