Two-step counterdiffusion protocol for the crystallization of haemoglobin II from Lucina pectinata in the pH range 4–9

Nieves-Marrero, C.A.; Ruiz-Martínez, C.R.; Estremera-Andújar, R.A.; González-Ramírez, L.A.; López-Garriga, J.; Gavira, J.A.

doi:10.1107/S1744309109053081

Acta Crystallographica Section F
Acta Crystallographica
Section F STRUCTURAL BIOLOGY COMMUNICATIONS

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Figure 1
(a) OxyHbII counterdiffusion behaviour in a 0.2 mm capillary. (b) The crystals grown from the GCB capillaries were extracted and equilibrated in 50 µl cryoprotectant solution. (c) A crystal of oxyHbII grown at pH 8.0, mounted on a nylon loop and flash-cooled in a 100 K liquid-nitrogen jetstream.

STRUCTURAL BIOLOGY
COMMUNICATIONS

ISSN: 2053-230X

Volume 66| Part 3| February 2010| Pages 264-268

doi:10.1107/S1744309109053081

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