The structure of BVU2987 from Bacteroides vulgatus reveals a superfamily of bacterial periplasmic proteins with possible inhibitory function

Das, D.; Finn, R.D.; Carlton, D.; Miller, M.D.; Abdubek, P.; Astakhova, T.; Axelrod, H.L.; Bakolitsa, C.; Chen, C.; Chiu, H.-J.; Chiu, M.; Clayton, T.; Deller, M.C.; Duan, L.; Ellrott, K.; Ernst, D.; Farr, C.L.; Feuerhelm, J.; Grant, J.C.; Grzechnik, A.; Han, G.W.; Jaroszewski, L.; Jin, K.K.; Klock, H.E.; Knuth, M.W.; Kozbial, P.; Krishna, S.S.; Kumar, A.; Marciano, D.; McMullan, D.; Morse, A.T.; Nigoghossian, E.; Nopakun, A.; Okach, L.; Puckett, C.; Reyes, R.; Rife, C.L.; Sefcovic, N.; Tien, H.J.; Trame, C.B.; van den Bedem, H.; Weekes, D.; Wooten, T.; Xu, Q.; Hodgson, K.O.; Wooley, J.; Elsliger, M.-A.; Deacon, A.M.; Godzik, A.; Lesley, S.A.; Wilson, I.A.

doi:10.1107/S1744309109046788

Acta Crystallographica Section F
Acta Crystallographica
Section F STRUCTURAL BIOLOGY COMMUNICATIONS

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Figure 1
Crystal structure of BVU2987 from B. vulgatus. (a) Stereo ribbon diagram of the BVU2987 monomer with the N-terminal domain in cyan and the C-terminal tandem-repeat domain in pink. Helices H1–H4 (helices H1 and H3 are 3₁₀-helices and helices H2 and H4 are α-helices) and β-strands β1–β8 are indicated. (b) Diagram showing the secondary-structural elements of BVU2987 superimposed on its primary sequence. The α-helices, 3₁₀-helices and β-strands are indicated. The crystallized protein (including residues 20–145) was expressed with a tag that was removed during purification, leaving Gly0 followed by the target sequence (starting from residue 20). (c) The electrostatic surface potential reveals a prominent negatively charged region on the concave side of BVU2987 arising from the presence of numerous aspartic acid and glutamic acid residues (Asp21, Asp22, Glu54, Asp56, Asp59, Asp63, Glu73, Glu82, Glu116, Asp118, Glu123, Glu131, Asp142 and Asp144). The color scale is in units of ±kT/e.

STRUCTURAL BIOLOGY
COMMUNICATIONS

ISSN: 2053-230X

Volume 66| Part 10| March 2010| Pages 1265-1273

doi:10.1107/S1744309109046788

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