Figure 14
NMR evidence for local conformational exchange in the NMR structure of A2LD1. (a) [15N,1H]-HSQC spectrum of a 1.1 mM solution of A2LD1 recorded at 700 MHz and 298 K. The cross-peaks of residues shown in (b) and (c) are identified. (b) Cross-sections along ω2(1H) through cross-peaks of active-site residues with low-frequency mobility (see text), as identified with the corresponding color code in (a). (c) The same as (b) for residues with high-frequency mobility (see text). |