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Figure 1
(a) SDS–PAGE analysis of the purified OST1 D160A and OST1 K50N mutants. The lane on the left corresponds to the GST-fusion protein and the lane on the right to the product after thrombin cleavage. The expected molecular weights are indicated. (b) The size-exclusion chromatogram of OST1 K50N and OST1 D160A mutants. The lines show the absorbance recorded at 280 nm. Molecular-weight markers (Bio-Rad) are indicated in kDa. |