Figure 1
2D [15N–1H]-HSQC spectrum of BobuA.00658.a recorded at pH 7.0 and 298 K. The spectrum was recorded on a Bruker Avance 600 MHz spectrometer with 1024 and 128 complex points along the t2 and t1 dimensions, respectively. The protein concentration was 1.4 mM in 93% H2O/7% 2H2O. The peaks are labeled with the single-letter amino-acid code followed by their respective sequence number, as established by sequence-specific assignments of the protein backbone. |