An example of an SDS–PAGE gel displaying the HTS results for 11 M. smegmatis target proteins. Reading from left to right, the first lane shows the protein ladder (in kDa). The next two lanes show the total (T) and IMAC elution (P) fractions of a positive control that is known to have high IMAC recovery. The following lanes represent a total of 11 target proteins of two lanes each, alternating between their T and P fractions. IMAC-recovery scores are determined by evaluating the size of each band (as described in §2.2.5), e.g. target s38 A03's recovery would be scored as low, s38 A06's recovery would be scored as medium and s38 A05's recovery would be scored as high. Targets s38 A09 and s38 A10 are insoluble and would not be queued for scale up in LSE.