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Figure 5
An example of an SDS–PAGE gel of LSE screens of eight expressed M. smegmatis target proteins. The two outermost lanes hold the protein ladders (labeled in kDa). Each target protein-expression preparation occupies three lanes: total expressed (T), soluble expressed (S) and IMAC elution pure (P) fractions. The variations in expression levels as seen in this gel are typical. The solubility-scoring system is identical to that of the HTS (as described in §[link]2.2.5). Target protein recoverability after IMAC would be scored as low for 2, 3 and 7, medium for 5 and 6, and high for 4 and 8. Target 1 is primarily insoluble and would not be queued for purification.

ISSN: 2053-230X
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